M M Dolmans1, M M Binda, S Jacobs, J P Dehoux, J L Squifflet, J Ambroise, J Donnez, C A Amorim. 1. Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200, Brussels, Belgium, Marie-Madeleine.Dolmans@uclouvain.be.
Abstract
PURPOSE: The aim of this study was to determine the best combination in terms of cryopreservation techniques and vascular bed preparation before grafting in order to obtain functional ovarian tissue after transplantation. METHODS: Five cynomolgus monkeys were used. Strips from 10 ovaries were cryopreserved, 5 by vitrification (V), and 5 by slow-freezing (SF). Pieces of fresh ovarian tissue were used for controls. After 1 month, the strips were autografted to two different vascular beds, healed (HB) or freshly decorticated (FDB), constituting four study groups: SF-HB, SF-FDB, V-HB, and V-FDB. These were compared to fresh tissue. After 6 months, the ovaries were removed and several parameters analyzed: follicle quality, stage, density, proliferation, apoptosis, functionality, vascularization, and fibrosis. Mixed effect linear regression models were built to assess the impact of cryopreservation and vascular bed preparation on ovarian tissue viability and functionality. p values were adjusted for multiple testing using the Benjamini-Hochberg method, and q values < 0.20 were considered significant in order to achieve a 20% false discovery rate. RESULTS: Compared to fresh tissue, no difference was observed in the percentage of morphologically normal follicles, while a significant increase was noted in the follicle proliferation rate (41%, q = 0.19), percentage of antral follicles (12%, q = 0.14), and number of vessels per area (3.3 times, q = 0.07) in the V-FDB group. CONCLUSIONS: Vitrification associated with FDB vascular bed preparation is the best combination to obtain functional autografted ovarian tissue. Further studies are nevertheless required, with confirmed pregnancies and live births before introducing the procedure into clinical practice.
PURPOSE: The aim of this study was to determine the best combination in terms of cryopreservation techniques and vascular bed preparation before grafting in order to obtain functional ovarian tissue after transplantation. METHODS: Five cynomolgus monkeys were used. Strips from 10 ovaries were cryopreserved, 5 by vitrification (V), and 5 by slow-freezing (SF). Pieces of fresh ovarian tissue were used for controls. After 1 month, the strips were autografted to two different vascular beds, healed (HB) or freshly decorticated (FDB), constituting four study groups: SF-HB, SF-FDB, V-HB, and V-FDB. These were compared to fresh tissue. After 6 months, the ovaries were removed and several parameters analyzed: follicle quality, stage, density, proliferation, apoptosis, functionality, vascularization, and fibrosis. Mixed effect linear regression models were built to assess the impact of cryopreservation and vascular bed preparation on ovarian tissue viability and functionality. p values were adjusted for multiple testing using the Benjamini-Hochberg method, and q values < 0.20 were considered significant in order to achieve a 20% false discovery rate. RESULTS: Compared to fresh tissue, no difference was observed in the percentage of morphologically normal follicles, while a significant increase was noted in the follicle proliferation rate (41%, q = 0.19), percentage of antral follicles (12%, q = 0.14), and number of vessels per area (3.3 times, q = 0.07) in the V-FDB group. CONCLUSIONS: Vitrification associated with FDB vascular bed preparation is the best combination to obtain functional autografted ovarian tissue. Further studies are nevertheless required, with confirmed pregnancies and live births before introducing the procedure into clinical practice.
Authors: Anu David; Anne Van Langendonckt; Sébastien Gilliaux; Marie-Madeleine Dolmans; Jacques Donnez; Christiani A Amorim Journal: Hum Reprod Date: 2012-02-07 Impact factor: 6.918
Authors: Christiani A Amorim; Mara Curaba; Anne Van Langendonckt; Marie-Madeleine Dolmans; Jacques Donnez Journal: Reprod Biomed Online Date: 2011-05-07 Impact factor: 3.828
Authors: Ariella Shikanov; Zheng Zhang; Min Xu; Rachel M Smith; Aniruddha Rajan; Teresa K Woodruff; Lonnie D Shea Journal: Tissue Eng Part A Date: 2011-09-21 Impact factor: 3.845
Authors: Christiani Andrade Amorim; Anu David; Anne Van Langendonckt; Marie-Madeleine Dolmans; Jacques Donnez Journal: Fertil Steril Date: 2010-12-18 Impact factor: 7.329
Authors: J Donnez; M M Dolmans; D Demylle; P Jadoul; C Pirard; J Squifflet; B Martinez-Madrid; A van Langendonckt Journal: Lancet Date: 2004 Oct 16-22 Impact factor: 79.321
Authors: Monica M Laronda; Kelly E McKinnon; Alison Y Ting; Ann V Le Fever; Mary B Zelinski; Teresa K Woodruff Journal: J Assist Reprod Genet Date: 2016-11-30 Impact factor: 3.412
Authors: Luciana Cacciottola; Thu Y T Nguyen; Maria C Chiti; Alessandra Camboni; Christiani A Amorim; Jacques Donnez; Marie-Madeleine Dolmans Journal: J Clin Med Date: 2020-09-15 Impact factor: 4.241