Distribution of cells in different stages of the cell cycle and some age-related physiological characteristics in dependence on the dilution rate in chemostat cultures of Candida utilis.

Position of cells in their cell cycle was determined microscopically in chemostat cultures of Candida utilis. Proportion of cells in phase G1 decreased in a linear manner from 86% to 58% with dilution rate. Proportion of cells in phase S increased in the same range of D from 5.6 to 13.5% and in the (G2 + M) phase from 8.4 to 28.5%, again linearly. Differential centrifugation was used to separate chemostat cultures to mother and daughter cells. Analyses showed that, relative to mother cells, daughter cells contain 2.1-11.9% more protein and 25.5-34.6% more RNA in dry matter. Their mass is 34.4-5.6% lower and volume is 154-19% smaller.