OBJECTIVE: To evaluate the possibility of detecting intestinal ischemia by intraluminal microdialysis and comparing the ileum and colon. METHODS: The studies were performed on male Sprague-Dawley rats. In the first part of the study, microdialysis catheters were placed in the sigmoid part of the colon and in the subcutaneous adipose tissue. In the second part of the study, microdialysis catheters were placed in the lumen of the ileum and the colon. The infrarenal aorta was clamped proximal to the cranial mesenteric artery. Microdialysate levels of glucose, lactate, pyruvate and glycerol were measured. Intestinal specimens were removed at the end of the ischemic period for microscopic evaluation. RESULTS: Intraluminal microdialysis could detect early signs of ischemic injury in the ileum, as well as in the colon, with a marked increase of lactate, lactate/pyruvate ratio and glycerol. The increased levels of intraluminal glycerol showed a positive correlation to prolonged ischemia and to higher degrees of intestinal damage. CONCLUSION: Intraluminal measurement of glycerol is a good marker for intestinal ischemia. Intraluminal microdialysis in the colon is easily accessible through the rectum, and may prove to be a valuable clinical tool for diagnosing intestinal ischemia.
OBJECTIVE: To evaluate the possibility of detecting intestinal ischemia by intraluminal microdialysis and comparing the ileum and colon. METHODS: The studies were performed on male Sprague-Dawley rats. In the first part of the study, microdialysis catheters were placed in the sigmoid part of the colon and in the subcutaneous adipose tissue. In the second part of the study, microdialysis catheters were placed in the lumen of the ileum and the colon. The infrarenal aorta was clamped proximal to the cranial mesenteric artery. Microdialysate levels of glucose, lactate, pyruvate and glycerol were measured. Intestinal specimens were removed at the end of the ischemic period for microscopic evaluation. RESULTS: Intraluminal microdialysis could detect early signs of ischemic injury in the ileum, as well as in the colon, with a marked increase of lactate, lactate/pyruvate ratio and glycerol. The increased levels of intraluminal glycerol showed a positive correlation to prolonged ischemia and to higher degrees of intestinal damage. CONCLUSION: Intraluminal measurement of glycerol is a good marker for intestinal ischemia. Intraluminal microdialysis in the colon is easily accessible through the rectum, and may prove to be a valuable clinical tool for diagnosing intestinal ischemia.
Authors: Lauri Pynnönen; Minna Minkkinen; Anders Perner; Sari Räty; Isto Nordback; Juhani Sand; Jyrki Tenhunen Journal: BMC Gastroenterol Date: 2013-12-10 Impact factor: 3.067
Authors: Runar J Strand-Amundsen; Henrik M Reims; Finn P Reinholt; Tom E Ruud; Runkuan Yang; Jan O Høgetveit; Tor I Tønnessen Journal: World J Gastroenterol Date: 2018-05-14 Impact factor: 5.742