OBJECTIVES: HIV and antiretroviral (ART) exposure in utero may have deleterious effects on the infant, but uncertainty still exists. The objective of this study was to evaluate aspects of mitochondrial DNA (mtDNA) content, mitochondrial function and oxidative stress simultaneously in placenta, umbilical cord blood and infant blood in HIV/ART-exposed infants compared with uninfected controls. METHODS: HIV-1-infected pregnant women and HIV-1-uninfected healthy pregnant controls were enrolled in the study prospectively. Placenta and umbilical cord blood were obtained at delivery and infant blood was obtained within 48 h of delivery. mtDNA content was determined for each specimen. Nuclear [subunit IV of cytochrome c-oxidase (COX IV)]- and mitochondrial (COX II)-encoded polypeptides of the oxidative phosphorylation enzyme cytochrome c-oxidase were quantified in cord and infant blood. Placental mitochondria malondialdehyde (MDA) concentrations were measured as a marker of oxidative stress. RESULTS: Twenty HIV-positive/HIV-exposed and 26 control mother-infant pairs were enrolled in the study. All HIV-infected women and their infants received ART. Placental MDA concentration and mtDNA content in placenta and cord blood were similar between groups. The cord blood COX II:IV ratio was lower in the HIV-positive group than in the controls, whereas the infant peripheral blood mtDNA content was higher in the HIV-exposed infants, but the infant peripheral blood COX II:IV ratio was similar. No infant had clinical evidence of mitochondrial disease or acquired HIV infection. In multivariable regression analyses, the significant findings in cord and infant blood were both most associated with HIV/ART exposure. CONCLUSIONS: HIV-exposed infants showed reduced umbilical cord blood mitochondrial enzyme expression with increased infant peripheral blood mitochondrial DNA levels, the latter possibly reflecting a compensatory mechanism to overcome HIV/ART-associated mitochondrial toxicity.
OBJECTIVES: HIV and antiretroviral (ART) exposure in utero may have deleterious effects on the infant, but uncertainty still exists. The objective of this study was to evaluate aspects of mitochondrial DNA (mtDNA) content, mitochondrial function and oxidative stress simultaneously in placenta, umbilical cord blood and infant blood in HIV/ART-exposed infants compared with uninfected controls. METHODS:HIV-1-infected pregnant women and HIV-1-uninfected healthy pregnant controls were enrolled in the study prospectively. Placenta and umbilical cord blood were obtained at delivery and infant blood was obtained within 48 h of delivery. mtDNA content was determined for each specimen. Nuclear [subunit IV of cytochrome c-oxidase (COX IV)]- and mitochondrial (COX II)-encoded polypeptides of the oxidative phosphorylation enzyme cytochrome c-oxidase were quantified in cord and infant blood. Placental mitochondria malondialdehyde (MDA) concentrations were measured as a marker of oxidative stress. RESULTS: Twenty HIV-positive/HIV-exposed and 26 control mother-infant pairs were enrolled in the study. All HIV-infectedwomen and their infants received ART. Placental MDA concentration and mtDNA content in placenta and cord blood were similar between groups. The cord blood COX II:IV ratio was lower in the HIV-positive group than in the controls, whereas the infant peripheral blood mtDNA content was higher in the HIV-exposed infants, but the infant peripheral blood COX II:IV ratio was similar. No infant had clinical evidence of mitochondrial disease or acquired HIV infection. In multivariable regression analyses, the significant findings in cord and infant blood were both most associated with HIV/ART exposure. CONCLUSIONS: HIV-exposed infants showed reduced umbilical cord blood mitochondrial enzyme expression with increased infant peripheral blood mitochondrial DNA levels, the latter possibly reflecting a compensatory mechanism to overcome HIV/ART-associated mitochondrial toxicity.
Authors: Rao L Divi; Kathryn J Haverkos; Juliette A Humsi; Marie E Shockley; Chandrasekhar Thamire; Kunio Nagashima; Ofelia A Olivero; Miriam C Poirier Journal: Environ Mol Mutagen Date: 2007 Apr-May Impact factor: 3.216
Authors: O A Olivero; G M Shearer; C A Chougnet; A A Kovacs; R Baker; A M Stek; M M Khoury; M C Poirier Journal: Ann N Y Acad Sci Date: 2000-11 Impact factor: 5.691
Authors: Grace M Aldrovandi; Clara Chu; William T Shearer; Daner Li; Jan Walter; Bruce Thompson; Kenneth McIntosh; Marc Foca; William A Meyer; Belinda F Ha; Kenneth C Rich; Jack Moye Journal: Pediatrics Date: 2009-11-23 Impact factor: 7.124
Authors: O A Olivero; L M Anderson; B A Diwan; D C Haines; S W Harbaugh; T J Moskal; A B Jones; J M Rice; C W Riggs; D Logsdon; S H Yuspa; M C Poirier Journal: J Natl Cancer Inst Date: 1997-11-05 Impact factor: 13.506
Authors: Hélène C F Côté; Janet Raboud; Ari Bitnun; Ariane Alimenti; Deborah M Money; Evelyn Maan; Adriana Costei; Izabelle Gadawski; Christina Diong; Stanley Read; Sandy Shen; P Richard Harrigan; David R Burdge; Susan M King; John C Forbes Journal: J Infect Dis Date: 2008-09-15 Impact factor: 5.226
Authors: Grace A McComsey; Minhee Kang; Allison C Ross; Dirk Lebrecht; Elizabeth Livingston; Ann Melvin; Jane Hitti; Susan E Cohn; Ulrich A Walker Journal: HIV Clin Trials Date: 2008 Mar-Apr
Authors: Miriam C Poirier; Alexander T Gibbons; Maria T Rugeles; Isabelle Andre-Schmutz; Stephane Blanche Journal: Curr Opin Pediatr Date: 2015-04 Impact factor: 2.856
Authors: Denise L Jacobson; Kunjal Patel; Paige L Williams; Mitchell E Geffner; George K Siberry; Linda A DiMeglio; Marilyn J Crain; Ayesha Mirza; Janet S Chen; Elizabeth McFarland; Deborah Kacanek; Margarita Silio; Kenneth Rich; William Borkowsky; Russell B Van Dyke; Tracie L Miller Journal: Pediatr Infect Dis J Date: 2017-02 Impact factor: 2.129
Authors: Abhinav Ajaykumar; Mayanne Zhu; Fatima Kakkar; Jason Brophy; Ari Bitnun; Ariane Alimenti; Hugo Soudeyns; Sara Saberi; Arianne Y K Albert; Deborah M Money; Hélène C F Côté Journal: J Infect Dis Date: 2021-02-24 Impact factor: 5.226
Authors: Jennifer Jao; Kathleen M Powis; Brian Kirmse; Chunli Yu; Fanny Epie; Emmanuel Nshom; Elaine J Abrams; Rhoda S Sperling; Derek Leroith; Mitchell E Geffner; Irwin J Kurland; Hélène C F Côté Journal: AIDS Date: 2017-11-28 Impact factor: 4.177
Authors: M López; M Palacio; A Goncé; S Hernàndez; F J Barranco; L García; M Loncà; J O Coll; E Gratacós; F Figueras Journal: Eur J Clin Microbiol Infect Dis Date: 2014-08-09 Impact factor: 3.267