Literature DB >> 22102045

Cloning, overexpression, purification, crystallization and preliminary X-ray analysis of 3-ketosteroid Δ(4)-(5α)-dehydrogenase from Rhodococcus jostii RHA1.

Niels van Oosterwijk1, Jan Knol, Lubbert Dijkhuizen, Robert van der Geize, Bauke W Dijkstra.   

Abstract

3-Ketosteroid dehydrogenases are flavoproteins which play key roles in steroid ring degradation. The enzymes are abundantly present in actinobacteria, including the catabolic powerhouse Rhodococcus jostii and the pathogenic species R. equi and Mycobacterium tuberculosis. The gene for 3-ketosteroid Δ(4)-(5α)-dehydrogenase [Δ(4)-(5α)-KSTD] from R. jostii RHA1 was cloned and overexpressed in Escherichia coli. His-tagged Δ(4)-(5α)-KSTD enzyme was purified by Ni(2+)-NTA affinity chromatography, anion-exchange chromatography and size-exclusion chromatography and was crystallized using the hanging-drop vapour-diffusion method. Seeding greatly improved the number of crystals obtained. The crystals belonged to space group C222(1), with unit-cell parameters a = 99.2, b = 114.3, c = 110.2 Å. Data were collected to a resolution of 1.6 Å.
© 2011 International Union of Crystallography. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 22102045      PMCID： PMC3212380          DOI： 10.1107/S1744309111028727

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  18 in total

1.  Structural and mechanistic mapping of a unique fumarate reductase.

Authors:  P Taylor; S L Pealing; G A Reid; S K Chapman; M D Walkinshaw
Journal:  Nat Struct Biol       Date:  1999-12

2.  Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.

Authors:  H R LEVY; P TALALAY
Journal:  J Biol Chem       Date:  1959-08       Impact factor: 5.157

3.  Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages.

Authors:  Vania Rosas-Magallanes; Gustavo Stadthagen-Gomez; Jean Rauzier; Luis B Barreiro; Ludovic Tailleux; Frédéric Boudou; Ruth Griffin; Jérome Nigou; Mary Jackson; Brigitte Gicquel; Olivier Neyrolles
Journal:  Infect Immun       Date:  2006-10-09       Impact factor: 3.441

4.  Essential tyrosine residues in 3-ketosteroid-delta(1)-dehydrogenase from Rhodococcus rhodochrous.

Authors:  C Fujii; S Morii; M Kadode; S Sawamoto; M Iwami; E Itagaki
Journal:  J Biochem       Date:  1999-10       Impact factor: 3.387

5.  Structure and mechanism of the flavocytochrome c fumarate reductase of Shewanella putrefaciens MR-1.

Authors:  D Leys; A S Tsapin; K H Nealson; T E Meyer; M A Cusanovich; J J Van Beeumen
Journal:  Nat Struct Biol       Date:  1999-12

6.  The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse.

Authors:  Michael P McLeod; René L Warren; William W L Hsiao; Naoto Araki; Matthew Myhre; Clinton Fernandes; Daisuke Miyazawa; Wendy Wong; Anita L Lillquist; Dennis Wang; Manisha Dosanjh; Hirofumi Hara; Anca Petrescu; Ryan D Morin; George Yang; Jeff M Stott; Jacqueline E Schein; Heesun Shin; Duane Smailus; Asim S Siddiqui; Marco A Marra; Steven J M Jones; Robert Holt; Fiona S L Brinkman; Keisuke Miyauchi; Masao Fukuda; Julian E Davies; William W Mohn; Lindsay D Eltis
Journal:  Proc Natl Acad Sci U S A       Date:  2006-10-09       Impact factor: 11.205

7.  A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.

Authors:  Robert Van der Geize; Katherine Yam; Thomas Heuser; Maarten H Wilbrink; Hirofumi Hara; Matthew C Anderton; Edith Sim; Lubbert Dijkhuizen; Julian E Davies; William W Mohn; Lindsay D Eltis
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-30       Impact factor: 11.205

8.  iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM.

Authors:  T Geoff G Battye; Luke Kontogiannis; Owen Johnson; Harold R Powell; Andrew G W Leslie
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18

9.  3-Keto-5alpha-steroid Delta(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism.

Authors:  Jan Knol; Karin Bodewits; Gerda I Hessels; Lubbert Dijkhuizen; Robert van der Geize
Journal:  Biochem J       Date:  2008-03-01       Impact factor: 3.857

10.  Overview of the CCP4 suite and current developments.

Authors:  Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18
View more
  3 in total

1.  Genetic differences in ksdD influence on the ADD/AD ratio of Mycobacterium neoaurum.

Authors:  Rili Xie; Yanbing Shen; Ning Qin; Yibo Wang; Liqiu Su; Min Wang
Journal:  J Ind Microbiol Biotechnol       Date:  2015-01-09       Impact factor: 3.346

2.  Structure and catalytic mechanism of 3-ketosteroid-Delta4-(5α)-dehydrogenase from Rhodococcus jostii RHA1 genome.

Authors:  Niels van Oosterwijk; Jan Knol; Lubbert Dijkhuizen; Robert van der Geize; Bauke W Dijkstra
Journal:  J Biol Chem       Date:  2012-07-24       Impact factor: 5.157

3.  Predicting the accumulation of storage compounds by Rhodococcus jostii RHA1 in the feast-famine growth cycles using genome-scale flux balance analysis.

Authors:  Mohammad Tajparast; Dominic Frigon
Journal:  PLoS One       Date:  2018-03-01       Impact factor: 3.240
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.