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Literature DB >> 220998

The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.

Abstract

The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 X 10(-4)M) reduces the cellular uptake of HP by 75% or more. The photosensitized inactivation is enhanced when the cells are suspended in D2O-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation. Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light.

Entities: Chemical Species

Mesh：

Substances：
Hematoporphyrins
Oxygen

Year: 1979 PMID： 220998 PMCID： PMC2009925 DOI： 10.1038/bjc.1979.72

Source DB: PubMed Journal: Br J Cancer ISSN： 0007-0920 Impact factor: 7.640

22 in total

1. Quantitative determination of porphyrin uptake by tumor tissue following parenteral administration.

Authors: J WINKELMAN; D S RASMUSSEN-TAXDAL
Journal: Bull Johns Hopkins Hosp Date: 1960-10

2. The use of a derivative of hematoporhyrin in tumor detection.

Authors: R L LIPSON; E J BALDES; A M OLSEN
Journal: J Natl Cancer Inst Date: 1961-01 Impact factor: 13.506

3. Microviscosity modulation during the cell cycle of neuroblastoma cells.

Authors: S W de Laat; P T van der Saag; M Shinitzky
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

4. Cell cycle characteristics of synchronized and asynchronous populations of human cells and effect of cooling of selected mitotic cells.

Authors: E O Pettersen; O Bakke; T Lindmo; R Oftebro
Journal: Cell Tissue Kinet Date: 1977-11

5. Cellular and nuclear volume during the cell cycle of NHIK 3025 cells.

Authors: H B Steen; T Lindmo
Journal: Cell Tissue Kinet Date: 1978-01

6. Protoporphyrin-sensitized photodynamic modification of proteins in isolated human red blood cell membranes.

Authors: T M Dubbelman; A F de Goeij; J van Steveninck
Journal: Photochem Photobiol Date: 1978-08 Impact factor: 3.421

7. The effect of chemical structure on the photosensitizing efficiencies of porphyrins.

Authors: G Cauzzo; G Gennari; G Jori; J D Spikes
Journal: Photochem Photobiol Date: 1977-04 Impact factor: 3.421

8. Retinal sensitized photodynamic damage to liposomes.

Authors: M Delmelle
Journal: Photochem Photobiol Date: 1978-09 Impact factor: 3.421

Review 9. Cellular responses to combinations of hyperthermia and radiation.

Authors: W C Dewey; L E Hopwood; S A Sapareto; L E Gerweck
Journal: Radiology Date: 1977-05 Impact factor: 11.105

10. Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.

Authors: T Christensen; J Moan; E Wibe; R Oftebro
Journal: Br J Cancer Date: 1979-01 Impact factor: 7.640

34 in total

10. Investigation of some antimicrobial procedures on the in vitro development of early murine embryos aimed toward developing methods for the disinfection of mammalian embryos prior to transfer.

Authors: A Bielanski; W C Hare
Journal: J In Vitro Fert Embryo Transf Date: 1991-02

The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin.

1. Quantitative determination of porphyrin uptake by tumor tissue following parenteral administration.

2. The use of a derivative of hematoporhyrin in tumor detection.

3. Microviscosity modulation during the cell cycle of neuroblastoma cells.

4. Cell cycle characteristics of synchronized and asynchronous populations of human cells and effect of cooling of selected mitotic cells.

5. Cellular and nuclear volume during the cell cycle of NHIK 3025 cells.

6. Protoporphyrin-sensitized photodynamic modification of proteins in isolated human red blood cell membranes.

7. The effect of chemical structure on the photosensitizing efficiencies of porphyrins.

8. Retinal sensitized photodynamic damage to liposomes.

Review 9. Cellular responses to combinations of hyperthermia and radiation.

10. Photodynamic effect of haematoporphyrin throughout the cell cycle of the human cell line NHIK 3025 cultivated in vitro.

1. Response of human endometrium and ovarian carcinoma cell-lines to photodynamic therapy.

2. Photodynamic effects of novel XF porphyrin derivatives on prokaryotic and eukaryotic cells.

Review 3. Photodynamic therapy in the treatment of cancer: current state of the art.

4. Porphyrin-sensitized photoinactivation of human cells in vitro.

5. Amino acid and energy requirements for rat hepatocytes in primary culture.

6. Light-induced protoporphyrin release from erythrocytes in erythropoietic protoporphyria.

7. Photochemical damage to skin fibroblasts caused by protoporphyrin and violet light.

8. Cellular effects of hematoporphyrin derivative photodynamic therapy on normal and neoplastic rat bladder cells.

9. Targeted killing of cultured cells by receptor-dependent photosensitization.

10. Investigation of some antimicrobial procedures on the in vitro development of early murine embryos aimed toward developing methods for the disinfection of mammalian embryos prior to transfer.