Literature DB >> 22094811

Rapid selection of high-affinity binders using ribosome display.

Birgit Dreier1, Andreas Plückthun.   

Abstract

Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail.

Mesh：

Substances：

Year:  2012        PMID： 22094811     DOI： 10.1007/978-1-61779-379-0_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  29 in total

1.  A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end.

Authors:  Ludovic Pecqueur; Christian Duellberg; Birgit Dreier; Qiyang Jiang; Chunguang Wang; Andreas Plückthun; Thomas Surrey; Benoît Gigant; Marcel Knossow
Journal:  Proc Natl Acad Sci U S A       Date:  2012-07-09       Impact factor: 11.205

Review 2.  Nanobodies as Probes for Protein Dynamics in Vitro and in Cells.

Authors:  Oleg Y Dmitriev; Svetlana Lutsenko; Serge Muyldermans
Journal:  J Biol Chem       Date:  2015-12-16       Impact factor: 5.157

3.  Conformation-dependent recognition of HIV gp120 by designed ankyrin repeat proteins provides access to novel HIV entry inhibitors.

Authors:  Axel Mann; Nikolas Friedrich; Anders Krarup; Jacqueline Weber; Emanuel Stiegeler; Birgit Dreier; Pavel Pugach; Melissa Robbiani; Tina Riedel; Kerstin Moehle; John A Robinson; Peter Rusert; Andreas Plückthun; Alexandra Trkola
Journal:  J Virol       Date:  2013-03-13       Impact factor: 5.103

4.  Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters.

Authors:  Birgit Dreier; Annemarie Honegger; Christian Hess; Gabriela Nagy-Davidescu; Peer R E Mittl; Markus G Grütter; Natalya Belousova; Galina Mikheeva; Victor Krasnykh; Andreas Plückthun
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-19       Impact factor: 11.205

Review 5.  Protein design: Past, present, and future.

Authors:  Lynne Regan; Diego Caballero; Michael R Hinrichsen; Alejandro Virrueta; Danielle M Williams; Corey S O'Hern
Journal:  Biopolymers       Date:  2015-07       Impact factor: 2.505

6.  Magnetic Bead-Immobilized Mammalian Cells Are Effective Targets to Enrich Ligand-Displaying Yeast.

Authors:  Patrick S Lown; Benjamin J Hackel
Journal:  ACS Comb Sci       Date:  2020-04-27       Impact factor: 3.784

7.  Ligand Engineering via Yeast Surface Display and Adherent Cell Panning.

Authors:  Lawrence A Stern; Patrick S Lown; Benjamin J Hackel
Journal:  Methods Mol Biol       Date:  2020

8.  Extended yeast surface display linkers enhance the enrichment of ligands in direct mammalian cell selections.

Authors:  Patrick S Lown; Jessy J Cai; Seth C Ritter; Jacob J Otolski; Ryan Wong; Benjamin J Hackel
Journal:  Protein Eng Des Sel       Date:  2021-02-15       Impact factor: 1.650

9.  Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process.

Authors:  Gillian Houlihan; Pietro Gatti-Lafranconi; David Lowe; Florian Hollfelder
Journal:  Protein Eng Des Sel       Date:  2015-06-30       Impact factor: 1.650

10.  Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5.

Authors:  Filip Radom; Clemens Vonrhein; Peer R E Mittl; Andreas Plückthun
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2021-06-29       Impact factor: 1.056
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.