Literature DB >> 2208651

Improved methods for genotype determination of human alcohol dehydrogenase (ADH) at ADH 2 and ADH 3 loci by using polymerase chain reaction-directed mutagenesis.

A Groppi1, J Begueret, A Iron.   

Abstract

The human gene for producing alcohol dehydrogenase (ADH; EC 1.1.1.1) is polymorphic at ADH 2 and ADH 3 loci. Until now, the study of this polymorphism required liver biopsy or allele-specific radioactive probes. We have used directed mutagenesis by the polymerase chain reaction (PCR) to amplify and analyze the genotype of ADH 2 and ADH 3 loci. Thus, we could determine easily and unambiguously the complete genotype at these two loci by using a microsample of blood and restriction fragment length polymorphism after DNA amplification by PCR.

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Year:  1990        PMID： 2208651

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  12 in total

1.  Association of aldehyde dehydrogenase 2 gene polymorphism with multiple oesophageal dysplasia in head and neck cancer patients.

Authors:  M Muto; Y Hitomi; A Ohtsu; S Ebihara; S Yoshida; H Esumi
Journal:  Gut       Date:  2000-08       Impact factor: 23.059

2.  Alcohol dehydrogenase genetic polymorphisms, low-to-moderate alcohol consumption, and risk of breast cancer.

Authors:  Kala Visvanathan; Rosa M Crum; Paul T Strickland; Xiaojun You; Ingo Ruczinski; Sonja I Berndt; Anthony J Alberg; Sandra C Hoffman; George W Comstock; Douglas A Bell; Kathy J Helzlsouer
Journal:  Alcohol Clin Exp Res       Date:  2007-03       Impact factor: 3.455

3.  First pass metabolism of ethanol is strikingly influenced by the speed of gastric emptying.

Authors:  C M Oneta; U A Simanowski; M Martinez; A Allali-Hassani; X Parés; N Homann; C Conradt; R Waldherr; W Fiehn; C Coutelle; H K Seitz
Journal:  Gut       Date:  1998-11       Impact factor: 23.059

4.  Allelic variation at alcohol metabolism genes ( ADH1B, ADH1C, ALDH2) and alcohol dependence in an American Indian population.

Authors:  Connie J Mulligan; Robert W Robin; Michael V Osier; Nyamkhishig Sambuughin; Lev G Goldfarb; Rick A Kittles; Diane Hesselbrock; David Goldman; Jeffrey C Long
Journal:  Hum Genet       Date:  2003-07-12       Impact factor: 4.132

5.  Increased cancer risk in heavy drinkers with the alcohol dehydrogenase 1C*1 allele, possibly due to salivary acetaldehyde.

Authors:  J P Visapää; K Götte; M Benesova; J Li; N Homann; C Conradt; H Inoue; M Tisch; K Hörrmann; S Väkeväinen; M Salaspuro; H K Seitz
Journal:  Gut       Date:  2004-06       Impact factor: 23.059

6.  Application of natural and amplification created restriction sites for the diagnosis of PKU mutations.

Authors:  H G Eiken; E Odland; H Boman; L Skjelkvåle; L F Engebretsen; J Apold
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

7.  Subtypes of ADH2 gene in alcoholics.

Authors:  P Chinnaswamy; V Vijayalakshmi
Journal:  Indian J Clin Biochem       Date:  2005-07

8.  Glutathione S-transferase class mu in French alcoholic cirrhotic patients.

Authors:  A Groppi; C Coutelle; B Fleury; A Iron; J Begueret; P Couzigou
Journal:  Hum Genet       Date:  1991-09       Impact factor: 4.132

9.  Alcohol-metabolizing enzyme gene polymorphisms and alcohol chronic pancreatitis among Polish individuals.

Authors:  Halina Cichoz-Lach; Krzysztof Celiński; Maria Słomka
Journal:  HPB (Oxford)       Date:  2008       Impact factor: 3.647

10.  Alcohol dehydrogenase genes: restriction fragment length polymorphisms for ADH4 (pi-ADH) and ADH5 (chi-ADH) and construction of haplotypes among different ADH classes.

Authors:  K Edman; W Maret
Journal:  Hum Genet       Date:  1992-12       Impact factor: 4.132
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