BACKGROUND AND AIMS: Chronic pancreatitis develops in 5-10% of alcohol addicts. In developed societies, alcohol is the cause of chronic pancreatitis in at least 70-80% of cases. The genetic polymorphism of enzymes involved in alcohol metabolism is relevant in the etiopathogenesis of chronic pancreatitis. The aim of the study was to find the ADH, ALDH2 and CYP2E1 alleles and genotypes in the Polish population that are likely to be responsible for higher susceptibility to chronic alcohol pancreatitis. MATERIAL AND METHODS: We determined the allele and genotype of ADH2, ADH3, ALDH2 and CYP2E1 in 141 subjects: 44 with alcohol chronic pancreatitis (ACP), 43 healthy alcoholics and 54 healthy non-drinkers as the controls. Genotyping was performed using PCR-RELP methods on white cell DNA. RESULTS: ADH2*1, ADH3*1 alleles and ADH2*1/*1, ADH3*1/*1 genotypes were statistically more frequent among the patients with ACP than among the controls. The ADH3*2/*2 genotype was more frequent among "healthy alcoholics" and in the controls than among those with ACP. In the studied group, only the ALDH2*1 allele was detected, all patients were ALDH2*1/*1 homozygotic. Differences in the CYP2E1 allele and genotype distribution in the examined groups were not significant. CONCLUSION: In the Polish population examined, ADH3*1 and ADH2*1 alleles may be risk factors for the development of alcoholism. The ADH3*2/*2 genotype may confer protection against ACP. CYP2E1 gene polymorphism is not related to alcoholism and ACP. The Polish population examined is ALDH2*1/*1 homozygotic.
BACKGROUND AND AIMS: Chronic pancreatitis develops in 5-10% of alcohol addicts. In developed societies, alcohol is the cause of chronic pancreatitis in at least 70-80% of cases. The genetic polymorphism of enzymes involved in alcohol metabolism is relevant in the etiopathogenesis of chronic pancreatitis. The aim of the study was to find the ADH, ALDH2 and CYP2E1 alleles and genotypes in the Polish population that are likely to be responsible for higher susceptibility to chronic alcohol pancreatitis. MATERIAL AND METHODS: We determined the allele and genotype of ADH2, ADH3, ALDH2 and CYP2E1 in 141 subjects: 44 with alcohol chronic pancreatitis (ACP), 43 healthy alcoholics and 54 healthy non-drinkers as the controls. Genotyping was performed using PCR-RELP methods on white cell DNA. RESULTS:ADH2*1, ADH3*1 alleles and ADH2*1/*1, ADH3*1/*1 genotypes were statistically more frequent among the patients with ACP than among the controls. The ADH3*2/*2 genotype was more frequent among "healthy alcoholics" and in the controls than among those with ACP. In the studied group, only the ALDH2*1 allele was detected, all patients were ALDH2*1/*1 homozygotic. Differences in the CYP2E1 allele and genotype distribution in the examined groups were not significant. CONCLUSION: In the Polish population examined, ADH3*1 and ADH2*1 alleles may be risk factors for the development of alcoholism. The ADH3*2/*2 genotype may confer protection against ACP. CYP2E1 gene polymorphism is not related to alcoholism and ACP. The Polish population examined is ALDH2*1/*1 homozygotic.
Authors: Michael V Osier; Andrew J Pakstis; Himla Soodyall; David Comas; David Goldman; Adekunle Odunsi; Friday Okonofua; Josef Parnas; Leslie O Schulz; Jaume Bertranpetit; Batsheva Bonne-Tamir; Ru-Band Lu; Judith R Kidd; Kenneth K Kidd Journal: Am J Hum Genet Date: 2002-06-05 Impact factor: 11.025
Authors: Andreas Frenzer; Wendy J Butler; Ian D Norton; Jeremy S Wilson; Minoti V Apte; Romano C Pirola; Philip Ryan; Ian C Roberts-Thomson Journal: J Gastroenterol Hepatol Date: 2002-02 Impact factor: 4.029
Authors: Mariette Verlaan; René H M Te Morsche; Hennie M J Roelofs; Robert J F Laheij; Jan B M J Jansen; Wilbert H M Peters; Joost P H Drenth Journal: Alcohol Alcohol Date: 2004 Jan-Feb Impact factor: 2.826
Authors: Mariette Verlaan; René H M te Morsche; Hennie M J Roelofs; Robert J F Laheij; Jan B M J Jansen; Wilbert H M Peters; Joost P H Drenth Journal: Am J Med Genet A Date: 2003-07-01 Impact factor: 2.802