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Literature DB >> 2206102

Isolation of Candida tropicalis auxotrophic mutants.

Abstract

An enrichment scheme using nystatin was designed for the isolation of auxotrophic mutants from the diploid-alkane-utilizing yeast Candida tropicalis. A collection of 194 auxotrophs representing 7 phenotypes was isolated. One class of mutants was identified as having a defect in histidinol dehydrogenase activity and a second class of mutants was identified as having a defect in orotidine monophosphate decarboxylase activity. These strains are good candidates to be carrying mutations corresponding to the HIS4 and URA3 genes of Saccharomyces cerevisiae.

Entities: Chemical Gene Species

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Year: 1990 PMID： 2206102 PMCID： PMC184766 DOI： 10.1128/aem.56.8.2562-2564.1990

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

17 in total

1. Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence.

Authors: K Okazaki; T Takechi; N Kambara; S Fukui; I Kubota; T Kamiryo
Journal: Proc Natl Acad Sci U S A Date: 1986-03 Impact factor: 11.205

10. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

Authors: J D Boeke; F LaCroute; G R Fink
Journal: Mol Gen Genet Date: 1984

1 in total

1. Transformation system for an asporogenous methylotrophic yeast, Candida boidinii: cloning of the orotidine-5'-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation.

Authors: Y Sakai; T Kazarimoto; Y Tani
Journal: J Bacteriol Date: 1991-12 Impact factor: 3.490

1 in total

Isolation of Candida tropicalis auxotrophic mutants.

1. Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence.

2. High-level expression and molecular cloning of genes encoding Candida tropicalis peroxisomal proteins.

3. Recombination after protoplast fusion in the yeast Candida tropicalis.

4. Genetical mutants induced by ethyl methanesulfonate in Saccharomyces.

5. Cloning of cDNA coding for peroxisomal acyl-CoA oxidase from the yeast Candida tropicalis pK233.

6. An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.

7. Fungemia in the immunocompromised host. Changing patterns, antigenemia, high mortality.

8. Pathogenicity of Candida tropicalis and Candida albicans after gastrointestinal inoculation in mice.

9. Pathologic features in the human alimentary tract associated with invasiveness of Candida tropicalis.

10. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.

1. Transformation system for an asporogenous methylotrophic yeast, Candida boidinii: cloning of the orotidine-5'-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation.