PURPOSE: The purpose of the study was to investigate the relationship between gas challenge-blood oxygen level-dependent (GC-BOLD) response angiogenesis and tumor size in rat Novikoff hepatoma model. MATERIALS AND METHODS: Twenty adult male Sprague-Dawley rats (weighting 301-325 g) were used for our Animal Care and Use Committee-approved experiments. N1-S1 Novikoff hepatomas were grown in 14 rats with sizes ranging from 0.42 to 2.81 cm. All experiments were performed at 3.0 T using a custom-built rodent receiver coil. A multiple gradient-echo sequence was used for R2* measurements, first during room air (78% N(2)/20% O(2)) breathing and then after 10 min of carbogen (95% O(2)/5% CO(2)) breathing. After image acquisition, rats were euthanized, and the tumors were harvested for histological evaluation. RESULTS: The R2* change between air and carbogen breathing for small hepatomas was positive; R2* changes changed to negative values for larger hepatomas. We found a significant positive correlation between tumor R2* change and tumor microvessel density (MVD) (r=0.798, P=.001) and a significant inverse correlation between tumor R2* change and tumor size (r=-0.840, P<.0001). CONCLUSIONS: GC-BOLD magnetic resonance imaging measurements are well correlated to MVD levels and tumor size in the N1-S1 Novikoff hepatoma model; GC-BOLD measurements may serve as noninvasive biomarkers for evaluating angiogenesis and disease progression and/or therapy response.
PURPOSE: The purpose of the study was to investigate the relationship between gas challenge-blood oxygen level-dependent (GC-BOLD) response angiogenesis and tumor size in ratNovikoff hepatoma model. MATERIALS AND METHODS: Twenty adult male Sprague-Dawley rats (weighting 301-325 g) were used for our Animal Care and Use Committee-approved experiments. N1-S1 Novikoff hepatomas were grown in 14 rats with sizes ranging from 0.42 to 2.81 cm. All experiments were performed at 3.0 T using a custom-built rodent receiver coil. A multiple gradient-echo sequence was used for R2* measurements, first during room air (78% N(2)/20% O(2)) breathing and then after 10 min of carbogen (95% O(2)/5% CO(2)) breathing. After image acquisition, rats were euthanized, and the tumors were harvested for histological evaluation. RESULTS: The R2* change between air and carbogen breathing for small hepatomas was positive; R2* changes changed to negative values for larger hepatomas. We found a significant positive correlation between tumor R2* change and tumor microvessel density (MVD) (r=0.798, P=.001) and a significant inverse correlation between tumor R2* change and tumor size (r=-0.840, P<.0001). CONCLUSIONS: GC-BOLD magnetic resonance imaging measurements are well correlated to MVD levels and tumor size in the N1-S1 Novikoff hepatoma model; GC-BOLD measurements may serve as noninvasive biomarkers for evaluating angiogenesis and disease progression and/or therapy response.
Authors: Ning Jin; Jie Deng; Tamuna Chadashvili; Yue Zhang; Yang Guo; Zhuoli Zhang; Guang-Yu Yang; Reed A Omary; Andrew C Larson Journal: Radiology Date: 2010-01 Impact factor: 11.105
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