| Literature DB >> 22037293 |
Marzena Poraj-Kobielska1, Matthias Kinne, René Ullrich, Katrin Scheibner, Martin Hofrichter.
Abstract
Rapid and simple spectrophotometric methods are required for the unambiguous detection of recently discovered fungal peroxygenases in vivo and in vitro. This paper describes a peroxygenase-specific assay using 5-nitro-1,3-benzodioxole as substrate. The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425 nm (ε(425)=9,700 M(-1) cm(-1)), and a red color when adjusted to pH >12, which can be measured in form of an end-point determination at 514 nm (ε(514)=11,400 M(-1) cm(-1)). The assay is suitable for detecting peroxygenase activities in complex growth media and environmental samples as well as for high-throughput screenings.Entities:
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Year: 2011 PMID： 22037293 DOI： 10.1016/j.ab.2011.10.009
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365