| Literature DB >> 2202149 |
Abstract
Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.Entities:
Mesh:
Substances:
Year: 1990 PMID: 2202149 DOI: 10.1016/0042-6822(90)90417-p
Source DB: PubMed Journal: Virology ISSN: 0042-6822 Impact factor: 3.616