Literature DB >> 22021238

Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

Shiela E Unkles1, Vicki F Symington, Zorica Kotur, Ye Wang, M Yaeesh Siddiqi, James R Kinghorn, Anthony D M Glass.   

Abstract

High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.

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Year:  2011        PMID： 22021238      PMCID： PMC3232726          DOI： 10.1128/EC.05199-11

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  35 in total

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6.  Two perfectly conserved arginine residues are required for substrate binding in a high-affinity nitrate transporter.

Authors:  Shiela E Unkles; Duncan A Rouch; Ye Wang; M Yaeesh Siddiqi; Anthony D M Glass; James R Kinghorn
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8.  Nitrite transport is mediated by the nitrite-specific high-affinity NitA transporter and by nitrate transporters NrtA, NrtB in Aspergillus nidulans.

Authors:  Ye Wang; Wenbin Li; Yaeesh Siddiqi; Vicki F Symington; James R Kinghorn; Shiela E Unkles; Anthony D M Glass
Journal:  Fungal Genet Biol       Date:  2007-10-05       Impact factor: 3.495

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5.  High-affinity nitrate/nitrite transporters NrtA and NrtB of Aspergillus nidulans exhibit high specificity and different inhibitor sensitivity.

Authors:  Naureen Akhtar; Eugenia Karabika; James R Kinghorn; Anthony D M Glass; Shiela E Unkles; Duncan A Rouch
Journal:  Microbiology       Date:  2015-04-08       Impact factor: 2.777

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  7 in total

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