PURPOSE: To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. METHODS: Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos. RESULTS: Irrespective of TSA treatment, POU5F1 expression was not detected until 8-16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control. CONCLUSION: The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome.
PURPOSE: To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. METHODS:Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos. RESULTS: Irrespective of TSA treatment, POU5F1 expression was not detected until 8-16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control. CONCLUSION: The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome.
Authors: I Wilmut; N Beaujean; P A de Sousa; A Dinnyes; T J King; L A Paterson; D N Wells; L E Young Journal: Nature Date: 2002-10-10 Impact factor: 49.962
Authors: Nathalie Beaujean; Geraldine Hartshorne; Jennifer Cavilla; Jane Taylor; John Gardner; Ian Wilmut; Richard Meehan; Lorraine Young Journal: Curr Biol Date: 2004-04-06 Impact factor: 10.834
Authors: W Dean; F Santos; M Stojkovic; V Zakhartchenko; J Walter; E Wolf; W Reik Journal: Proc Natl Acad Sci U S A Date: 2001-11-20 Impact factor: 11.205
Authors: B Oback; A T Wiersema; P Gaynor; G Laible; F C Tucker; J E Oliver; A L Miller; H E Troskie; K L Wilson; J T Forsyth; M C Berg; K Cockrem; V McMillan; H R Tervit; D N Wells Journal: Cloning Stem Cells Date: 2003