| Literature DB >> 22015697 |
Natalia L P Iorio1, Milena B Azevedo, Vanessa H Frazão, Ariane G Barcellos, Elaine M Barros, Eliezer M Pereira, Cláudio S de Mattos, Kátia R N dos Santos.
Abstract
Staphylococcus epidermidis is the most prevalent coagulase-negative Staphylococcus (CNS) and is a major cause of hospital bacteremia. Based on 18 reference strains and 149 Staphylococcus clinical strains, used in a novel multiplex PCR method, the aim of this study was to identify S. epidermidis with respect to the sequence of three genes: recN, which encodes a recombination/repair protein, mecA (methicillin resistance), and icaAB, which is involved in biofilm formation. Amplicons of 219 bp (S. epidermidis-recN gene), 154 bp (mecA gene), and 546 bp (icaAB genes) were obtained. Reliable results were achieved for 100% of the evaluated strains, suggesting that this new multiplex-PCR approach could be useful for the accurate identification of methicillin-resistant S. epidermidis with the potential to produce biofilm.Entities:
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Year: 2011 PMID： 22015697 DOI： 10.2436/20.1501.01.130
Source DB: PubMed Journal: Int Microbiol ISSN： 1139-6709 Impact factor: 2.479