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Literature DB >> 2200337

Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: feasibility of 1-day Salmonella detection.

H A Lee¹, G M Wyatt, S Bramham, M R Morgan.

Abstract

A microtitration plate, antibody-capture, enzyme-linked immunosorbent assay was developed for detection of Salmonella typhimurium. The assay utilizes a monoclonal detector antibody which shows no cross-reactions with non-Salmonella species and only a slight cross-reaction with one other Salmonella serotype. By using only one cultural stage (in a nonselective, chemically defined medium) prior to the enzyme-linked immunosorbent assay, low numbers of cells in food (10 cells 25 g-1) were detected in 19 h. Non-Salmonella competing organisms did not interfere with detection of S. typhimurium even when present in the ratio of 10(6):1 (non-Salmonella/Salmonella spp.). The assay shows the feasibility of rapid, 1-day testing for Salmonella spp. with antibody technology.

Entities: Disease Species

Mesh：

Substances：

Year: 1990 PMID： 2200337 PMCID： PMC184468 DOI： 10.1128/aem.56.6.1541-1546.1990

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

14 in total

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10 in total

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Authors: Luciana Croci; Elisabetta Delibato; Giulia Volpe; Dario De Medici; Giuseppe Palleschi
Journal: Appl Environ Microbiol Date: 2004-03 Impact factor: 4.792

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Authors: Devendra Bhandari; Fur-Chi Chen; Roger C Bridgman
Journal: Sensors (Basel) Date: 2022-01-09 Impact factor: 3.576