Literature DB >> 21998420

Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR.

Anne Dubouix-Bourandy1, Aymard de Ladoucette, Valerie Pietri, Nazim Mehdi, David Benzaquen, Régis Guinand, Jean-Marc Gandois.   

Abstract

We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.

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Year:  2011        PMID： 21998420      PMCID： PMC3232950          DOI： 10.1128/JCM.00334-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  43 in total

1.  Using a combination of reference tests to assess the accuracy of a new diagnostic test.

Authors:  T A Alonzo; M S Pepe
Journal:  Stat Med       Date:  1999-11-30       Impact factor: 2.373

Review 2.  Applications of polymerase chain reaction in rheumatology.

Authors:  Raquel Cuchacovich; Stephen Quinet; Ana Maria Santos
Journal:  Rheum Dis Clin North Am       Date:  2003-02       Impact factor: 2.670

3.  Sonication of explanted prosthetic components in bags for diagnosis of prosthetic joint infection is associated with risk of contamination.

Authors:  Andrej Trampuz; Kerryl E Piper; Arlen D Hanssen; Douglas R Osmon; Franklin R Cockerill; James M Steckelberg; Robin Patel
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948

Review 4.  Infectious arthritis.

Authors:  J W Smith
Journal:  Infect Dis Clin North Am       Date:  1990-09       Impact factor: 5.982

5.  Improved diagnosis of periprosthetic joint infection by multiplex PCR of sonication fluid from removed implants.

Authors:  Yvonne Achermann; Markus Vogt; Michael Leunig; Jürg Wüst; Andrej Trampuz
Journal:  J Clin Microbiol       Date:  2010-02-17       Impact factor: 5.948

6.  Risk factors for septic arthritis in patients with joint disease. A prospective study.

Authors:  C J Kaandorp; D Van Schaardenburg; P Krijnen; J D Habbema; M A van de Laar
Journal:  Arthritis Rheum       Date:  1995-12

7.  Gentamicin release from old cement during revision hip arthroplasty.

Authors:  J W Powles; R F Spencer; A M Lovering
Journal:  J Bone Joint Surg Br       Date:  1998-07

8.  Culture-negative prosthetic joint infection.

Authors:  Elie F Berbari; Camelia Marculescu; Irene Sia; Brian D Lahr; Arlen D Hanssen; James M Steckelberg; Rachel Gullerud; Douglas R Osmon
Journal:  Clin Infect Dis       Date:  2007-09-26       Impact factor: 9.079

9.  Prospective comparative study of patients with culture proven and high suspicion of adult onset septic arthritis.

Authors:  M N Gupta; R D Sturrock; M Field
Journal:  Ann Rheum Dis       Date:  2003-04       Impact factor: 19.103

10.  Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study.

Authors:  Stephan Harbarth; Cristina Masuet-Aumatell; Jacques Schrenzel; Patrice Francois; Christophe Akakpo; Gesuele Renzi; Jerome Pugin; Bara Ricou; Didier Pittet
Journal:  Crit Care       Date:  2006-02       Impact factor: 9.097
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  18 in total

Review 1.  A Real Pain: Diagnostic Quandaries and Septic Arthritis.

Authors:  Cristina Costales; Susan M Butler-Wu
Journal:  J Clin Microbiol       Date:  2018-01-24       Impact factor: 5.948

2.  Focus on MRSA/SA SSTI Assay Failure in Prosthetic Joint Infections: 213 Consecutive Patients Later.

Authors:  Marie Titécat; Frédéric Wallet; Olivier Robineau; Michel Valette; Henri Migaud; Eric Senneville; Caroline Loïez
Journal:  J Clin Microbiol       Date:  2016-11-16       Impact factor: 5.948

3.  Rapid MRSA PCR on respiratory specimens from ventilated patients with suspected pneumonia: a tool to facilitate antimicrobial stewardship.

Authors:  S E Trevino; M A Pence; J Marschall; M H Kollef; H M Babcock; C-A D Burnham
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-12-21       Impact factor: 3.267

4.  Preoperative Staphylococcus aureus Carriage and Risk of Surgical Site Infection After Cardiac Surgery in Children Younger than 1 year: A Pilot Cohort Study.

Authors:  J Macher; C Gras Le Guen; A Chenouard; J M Liet; B Gaillard Le Roux; A Legrand; J Mahuet; E Launay; V Gournay; N Joram
Journal:  Pediatr Cardiol       Date:  2016-11-14       Impact factor: 1.655

5.  Rapid detection of Staphylococcus aureus in lower respiratory tract secretions from patients with suspected ventilator-associated pneumonia: evaluation of the Cepheid Xpert MRSA/SA SSTI assay.

Authors:  Emilia Cercenado; Mercedes Marín; Almudena Burillo; Pablo Martín-Rabadán; Marisa Rivera; Emilio Bouza
Journal:  J Clin Microbiol       Date:  2012-09-19       Impact factor: 5.948

6.  Evaluation of the MRSA/SA ELITe MGB Assay for the Detection of Staphylococcus aureus in Bone and Joint Infections.

Authors:  R Labetoulle; J Rigaill; P O Verhoeven; A Carricajo; M Lleres-Vadeboin; F Grattard; B Pozzetto; C Cazorla; E Botelho-Nevers; B Boyer; C Dupieux-Chabert; F Laurent
Journal:  J Clin Microbiol       Date:  2021-11-17       Impact factor: 11.677

7.  A guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM)(a).

Authors:  Ellen Jo Baron; J Michael Miller; Melvin P Weinstein; Sandra S Richter; Peter H Gilligan; Richard B Thomson; Paul Bourbeau; Karen C Carroll; Sue C Kehl; W Michael Dunne; Barbara Robinson-Dunn; Joseph D Schwartzman; Kimberle C Chapin; James W Snyder; Betty A Forbes; Robin Patel; Jon E Rosenblatt; Bobbi S Pritt
Journal:  Clin Infect Dis       Date:  2013-07-10       Impact factor: 9.079

8.  Direct molecular versus culture-based assessment of Gram-positive cocci in biopsies of patients with major abscesses and diabetic foot infections.

Authors:  M H T Stappers; F Hagen; P Reimnitz; J W Mouton; J F Meis; I C Gyssens
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2015-07-05       Impact factor: 3.267

Review 9.  Challenging Methicillin Resistance Detection in Bone and Joint Infections: Focus on the MRSA/SA SSTI® Strategy.

Authors:  Marie Titécat; Caroline Loïez; François Demaeght; Jean-Thomas Leclerc; Théo Martin; Hervé Dezèque; Henri Migaud; Eric Senneville
Journal:  Front Med (Lausanne)       Date:  2021-05-17

Review 10.  Periprosthetic joint infections: clinical and bench research.

Authors:  Laurence Legout; Eric Senneville
Journal:  ScientificWorldJournal       Date:  2013-10-27
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