Literature DB >> 22993185

Rapid detection of Staphylococcus aureus in lower respiratory tract secretions from patients with suspected ventilator-associated pneumonia: evaluation of the Cepheid Xpert MRSA/SA SSTI assay.

Emilia Cercenado1, Mercedes Marín, Almudena Burillo, Pablo Martín-Rabadán, Marisa Rivera, Emilio Bouza.   

Abstract

A preclinical evaluation was conducted to evaluate the performance of the Cepheid Xpert assay on 135 lower respiratory tract secretions for detection of methicillin-resistant Staphylococcus aureus and S. aureus. Compared with the quantitative culture, the sensitivity, specificity, and positive and negative predictive values were 99.0%, 72.2%, 90.7%, and 96.3%, respectively.

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Mesh：

Year:  2012        PMID： 22993185      PMCID： PMC3502952          DOI： 10.1128/JCM.02409-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus.

Authors:  Duarte C Oliveira; Hermínia de Lencastre
Journal:  Antimicrob Agents Chemother       Date:  2002-07       Impact factor: 5.191

2.  Quantitative tracheal lavage versus bronchoscopic protected specimen brush for the diagnosis of nosocomial pneumonia in mechanically ventilated patients.

Authors:  John A Aucar; Miguel Bongera; Jeffrey O Phillips; Ravishankar Kamath; Michael H Metzler
Journal:  Am J Surg       Date:  2003-12       Impact factor: 2.565

3.  Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.

Authors:  Kunyan Zhang; Jo-Ann McClure; Sameer Elsayed; Thomas Louie; John M Conly
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

4.  Be aware of the possibility of false-positive results in single-locus PCR assays for methicillin-resistant Staphylococcus aureus.

Authors:  Jan Rupp; Ines Fenner; Werner Solbach; Jens Gieffers
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

5.  Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements.

Authors:  Piriyaporn Chongtrakool; Teruyo Ito; Xiao Xue Ma; Yoko Kondo; Suwanna Trakulsomboon; Chuntima Tiensasitorn; Mantana Jamklang; Tavinun Chavalit; Jae-Hoon Song; Keiichi Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  2006-03       Impact factor: 5.191

Review 6.  Attributable mortality of ventilator-associated pneumonia.

Authors:  Jean-François Timsit; Jean-Ralph Zahar; Sylvie Chevret
Journal:  Curr Opin Crit Care       Date:  2011-10       Impact factor: 3.687

Review 7.  An unmet medical need: rapid molecular diagnostics tests for respiratory tract infections.

Authors: 
Journal:  Clin Infect Dis       Date:  2011-05       Impact factor: 9.079

8.  Ventilator-associated pneumonia due to meticillin-resistant Staphylococcus aureus: risk factors and outcome in a large general hospital.

Authors:  E Bouza; M Giannella; E Bunsow; M V Torres; M J Pérez Granda; P Martín-Rabadán; P Muñoz
Journal:  J Hosp Infect       Date:  2012-01-04       Impact factor: 3.926

Review 9.  Ventilator-associated pneumonia.

Authors:  Jean Chastre; Jean-Yves Fagon
Journal:  Am J Respir Crit Care Med       Date:  2002-04-01       Impact factor: 21.405

10.  Clinical importance of delays in the initiation of appropriate antibiotic treatment for ventilator-associated pneumonia.

Authors:  Manuel Iregui; Suzanne Ward; Glenda Sherman; Victoria J Fraser; Marin H Kollef
Journal:  Chest       Date:  2002-07       Impact factor: 9.410
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  21 in total

Review 1.  Better tests, better care: improved diagnostics for infectious diseases.

Authors:  Angela M Caliendo; David N Gilbert; Christine C Ginocchio; Kimberly E Hanson; Larissa May; Thomas C Quinn; Fred C Tenover; David Alland; Anne J Blaschke; Robert A Bonomo; Karen C Carroll; Mary Jane Ferraro; Lisa R Hirschhorn; W Patrick Joseph; Tobi Karchmer; Ann T MacIntyre; L Barth Reller; Audrey F Jackson
Journal:  Clin Infect Dis       Date:  2013-12       Impact factor: 9.079

2.  What's new in ventilator-associated pneumonia?

Authors:  Jordi Rello; Jordi Riera; Ricardo Serrano
Journal:  Intensive Care Med       Date:  2015-06-11       Impact factor: 17.440

3.  Rapid MRSA PCR on respiratory specimens from ventilated patients with suspected pneumonia: a tool to facilitate antimicrobial stewardship.

Authors:  S E Trevino; M A Pence; J Marschall; M H Kollef; H M Babcock; C-A D Burnham
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-12-21       Impact factor: 3.267

4.  Rapid Detection of Methicillin-Resistant Staphylococcus aureus in BAL: A Pilot Randomized Controlled Trial.

Authors:  Joseph R Paonessa; Raj D Shah; Chiagozie I Pickens; Bryan D Lizza; Helen K Donnelly; Michael Malczynski; Chao Qi; Richard G Wunderink
Journal:  Chest       Date:  2019-02-15       Impact factor: 9.410

5.  Performance of the Cepheid Methicillin-Resistant Staphylococcus aureus/S. aureus Skin and Soft Tissue Infection PCR Assay on Respiratory Samples from Mechanically Ventilated Patients for S. aureus Screening during the Phase 2 Double-Blind SAATELLITE Study.

Authors:  Alexey Ruzin; Olivier Barraud; Li Yu; Bruno François; Miguel Sánchez-Garcia; Philippe Eggimann; Pierre-François Dequin; Pierre-François Laterre; Vincent Huberlant; Lucia Viña; Thierry Boulain; Cedric Bretonniere; Jérôme Pugin; José Trenado; Ana Catalina Hernandez Padilla; Julie Vignaud; Drieke Vandamme; Herman Goossens; Christine Lammens; S Omar Ali; Kathryn Shoemaker; Pin Ren; Susan Colbert; Terramika Bellamy; Bret R Sellman; Michael McCarthy; Hasan S Jafri; Mark T Esser
Journal:  J Clin Microbiol       Date:  2022-06-27       Impact factor: 11.677

Review 6.  Sepsis: frontiers in diagnosis, resuscitation and antibiotic therapy.

Authors:  Anders Perner; Anthony C Gordon; Daniel De Backer; George Dimopoulos; James A Russell; Jeffrey Lipman; Jens-Ulrik Jensen; John Myburgh; Mervyn Singer; Rinaldo Bellomo; Timothy Walsh
Journal:  Intensive Care Med       Date:  2016-10-01       Impact factor: 17.440

Review 7.  Urgent need for a rapid microbiological diagnosis in critically ill pneumonia.

Authors:  F Martínez Sagasti; M Calle Romero; M Rodríguez Gómez; P Alonso Martínez; S C García-Perrote
Journal:  Rev Esp Quimioter       Date:  2022-04-22       Impact factor: 2.515

8.  Direct detection of methicillin-resistant Staphylococcus aureus in sputum specimens from patients with hospital-associated pneumonia using a novel multilocus PCR assay.

Authors:  Zeng-Guang Huang; Xing-Zheng Zheng; Jing Guan; Shu-Nian Xiao; Chao Zhuo
Journal:  Pathogens       Date:  2015-04-30

9.  Accuracy of Molecular Amplification Assays for Diagnosis of Staphylococcal Pneumonia: a Systematic Review and Meta-analysis.

Authors:  Ke Chen; Sarfraz Ahmed; Changfeng Sun; Yun-Jian Sheng; Gang Wu; Cun-Liang Deng; Suvash Chandra Ojha
Journal:  J Clin Microbiol       Date:  2021-07-19       Impact factor: 5.948

Review 10.  Revolutionising bacteriology to improve treatment outcomes and antibiotic stewardship.

Authors:  David M Livermore; John Wain
Journal:  Infect Chemother       Date:  2013-03-29
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