Literature DB >> 21996097

Quadriplex real-time polymerase chain reaction (lytA, mef, erm, pbp2b(wt)) for pneumococcal detection and assessment of antibiotic susceptibility.

Velusamy Srinivasan1, Mignon du Plessis, Bernard W Beall, Lesley McGee.   

Abstract

A quadriplex real-time polymerase chain reaction assay was developed for detecting pneumococci, penicillin susceptibility, and macrolide/lincosamide resistance. The assay was sensitive for all 4 targets (<10 copies) and correlated with antimicrobial susceptibilities in 172/180 isolates and 28/29 culture-positive clinical specimens. For 29 lytA-positive culture-negative specimens, the assay allowed interpretation of antimicrobial susceptibility. Published by Elsevier Inc.

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Year:  2011        PMID： 21996097     DOI： 10.1016/j.diagmicrobio.2011.08.017

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  7 in total

1.  Serotypes and Antimicrobial Susceptibility of Nasopharyngeal Isolates of Streptococcus pneumoniae from Children Less Than 5 Years Old in Egypt.

Authors:  Amani El-Kholy; Magda Badawy; Maha Gad; May Soliman
Journal:  Infect Drug Resist       Date:  2020-10-19       Impact factor: 4.003

2.  Multiplex real-time PCR using SYBR Green: Unspecific intercalating dye to detect antimicrobial resistance genes of Streptococcus pneumoniae in cerebrospinal fluid.

Authors:  Mariana Brena Souza; Maria Cecília Cergole-Novella; Delma Aparecida Molinari; Daniela Rodrigues Colpas; Andréia Moreira Dos Santos Carmo; Vilma Dos Santos Menezes Gaiotto Daros; Ivana Barros de Campos
Journal:  PLoS One       Date:  2022-06-14       Impact factor: 3.752

Review 3.  Microbial Resistance to Antibiotics and Effective Antibiotherapy.

Authors:  Adriana Aurelia Chiș; Luca Liviu Rus; Claudiu Morgovan; Anca Maria Arseniu; Adina Frum; Andreea Loredana Vonica-Țincu; Felicia Gabriela Gligor; Maria Lucia Mureșan; Carmen Maximiliana Dobrea
Journal:  Biomedicines       Date:  2022-05-12

Review 4.  Biological and Epidemiological Features of Antibiotic-Resistant Streptococcus pneumoniae in Pre- and Post-Conjugate Vaccine Eras: a United States Perspective.

Authors:  Lindsay Kim; Lesley McGee; Sara Tomczyk; Bernard Beall
Journal:  Clin Microbiol Rev       Date:  2016-07       Impact factor: 26.132

5.  Impact of Long-Term Erythromycin Therapy on the Oropharyngeal Microbiome and Resistance Gene Reservoir in Non-Cystic Fibrosis Bronchiectasis.

Authors:  Jocelyn M Choo; Guy C J Abell; Rachel Thomson; Lucy Morgan; Grant Waterer; David L Gordon; Steven L Taylor; Lex E X Leong; Steve L Wesselingh; Lucy D Burr; Geraint B Rogers
Journal:  mSphere       Date:  2018-04-18       Impact factor: 4.389

6.  Comparison of Culture, Antigen Test, and Polymerase Chain Reaction for Pneumococcal Detection in Cerebrospinal Fluid of Children.

Authors:  Md Hasanuzzaman; Senjuti Saha; Roly Malaker; Hafizur Rahman; Mohammad S I Sajib; Rajib C Das; Maksuda Islam; Davidson H Hamer; Gary L Darmstadt; Samir K Saha
Journal:  J Infect Dis       Date:  2021-09-01       Impact factor: 5.226

7.  Rapid Changes in Nasopharyngeal Antibiotic Resistance Gene Profiles After Short Courses of Antibiotics in a Pilot Study of Ambulatory Young Children.

Authors:  Leigh M Howard; Keerti L Dantuluri; Nicole Soper; Isaac P Thomsen; Carlos G Grijalva
Journal:  Open Forum Infect Dis       Date:  2021-10-16       Impact factor: 3.835
  7 in total

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