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Literature DB >> 21984185

miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT.

Marc R Fabian¹, Maja K Cieplak, Filipp Frank, Masahiro Morita, Jonathan Green, Tharan Srikumar, Bhushan Nagar, Tadashi Yamamoto, Brian Raught, Thomas F Duchaine, Nahum Sonenberg.

Abstract

miRNAs recruit the miRNA-induced silencing complex (miRISC), which includes Argonaute and GW182 as core proteins. GW182 proteins effect translational repression and deadenylation of target mRNAs. However, the molecular mechanisms of GW182-mediated repression remain obscure. We show here that human GW182 independently interacts with the PAN2-PAN3 and CCR4-NOT deadenylase complexes. Interaction of GW182 with CCR4-NOT is mediated by two newly discovered phylogenetically conserved motifs. Although either motif is sufficient to bind CCR4-NOT, only one of them can promote processive deadenylation of target mRNAs. Thus, GW182 serves as both a platform that recruits deadenylases and as a deadenylase coactivator that facilitates the removal of the poly(A) tail by CCR4-NOT.

Entities: Gene Species

Mesh：

Substances：

Year: 2011 PMID： 21984185 DOI： 10.1038/nsmb.2149

Source DB: PubMed Journal: Nat Struct Mol Biol ISSN： 1545-9985 Impact factor: 15.369

42 in total

1. MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F.

Authors: Géraldine Mathonnet; Marc R Fabian; Yuri V Svitkin; Armen Parsyan; Laurent Huck; Takayuki Murata; Stefano Biffo; William C Merrick; Edward Darzynkiewicz; Ramesh S Pillai; Witold Filipowicz; Thomas F Duchaine; Nahum Sonenberg
Journal: Science Date: 2007-07-26 Impact factor: 47.728

2. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells.

Authors: Xianghua Piao; Xue Zhang; Ligang Wu; Joel G Belasco
Journal: Mol Cell Biol Date: 2010-01-11 Impact factor: 4.272

3. Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex.

Authors: Takashi Miyasaka; Masahiro Morita; Kentaro Ito; Toru Suzuki; Hiroyuki Fukuda; Shizu Takeda; Jun-Ichiro Inoue; Kentaro Semba; Tadashi Yamamoto
Journal: Cancer Sci Date: 2008-04 Impact factor: 6.716

4. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay.

Authors: Ana Eulalio; Eric Huntzinger; Elisa Izaurralde
Journal: Nat Struct Mol Biol Date: 2008-03-16 Impact factor: 15.369

5. The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression.

Authors: Marina Chekulaeva; Roy Parker; Witold Filipowicz
Journal: Nucleic Acids Res Date: 2010-06-08 Impact factor: 16.971

6. Mammalian GW182 contains multiple Argonaute-binding sites and functions in microRNA-mediated translational repression.

Authors: Koji Takimoto; Motoaki Wakiyama; Shigeyuki Yokoyama
Journal: RNA Date: 2009-04-27 Impact factor: 4.942

7. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.

Authors: Shang L Lian; Songqing Li; Grant X Abadal; Brad A Pauley; Marvin J Fritzler; Edward K L Chan
Journal: RNA Date: 2009-03-26 Impact factor: 4.942

8. Functional dissection of the human TNRC6 (GW182-related) family of proteins.

Authors: David Baillat; Ramin Shiekhattar
Journal: Mol Cell Biol Date: 2009-05-26 Impact factor: 4.272

9. The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing.

Authors: Ana Eulalio; Felix Tritschler; Regina Büttner; Oliver Weichenrieder; Elisa Izaurralde; Vincent Truffault
Journal: Nucleic Acids Res Date: 2009-03-18 Impact factor: 16.971

10. A specific role for the C-terminal region of the Poly(A)-binding protein in mRNA decay.

Authors: Ernesto Simón; Bertrand Séraphin
Journal: Nucleic Acids Res Date: 2007-08-30 Impact factor: 16.971

163 in total

1. Translational inhibition by deadenylation-independent mechanisms is central to microRNA-mediated silencing in zebrafish.

Authors: Yuichiro Mishima; Akira Fukao; Tomoyoshi Kishimoto; Hiroshi Sakamoto; Toshinobu Fujiwara; Kunio Inoue
Journal: Proc Natl Acad Sci U S A Date: 2012-01-09 Impact factor: 11.205

miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT.

1. MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F.

2. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells.

3. Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex.

4. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay.

5. The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression.

6. Mammalian GW182 contains multiple Argonaute-binding sites and functions in microRNA-mediated translational repression.

7. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.

8. Functional dissection of the human TNRC6 (GW182-related) family of proteins.

9. The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing.

10. A specific role for the C-terminal region of the Poly(A)-binding protein in mRNA decay.

1. Translational inhibition by deadenylation-independent mechanisms is central to microRNA-mediated silencing in zebrafish.

Review 2. MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship.

Review 3. General principals of miRNA biogenesis and regulation in the brain.

Review 4. Translational control by changes in poly(A) tail length: recycling mRNAs.

Review 5. The mechanics of miRNA-mediated gene silencing: a look under the hood of miRISC.

Review 6. Inhibition and avoidance of mRNA degradation by RNA viruses.

7. Elucidating the temporal order of silencing.

Review 8. P-bodies and stress granules: possible roles in the control of translation and mRNA degradation.

9. Non-inhibited miRNAs shape the cellular response to anti-miR.

10. Structural features of Argonaute-GW182 protein interactions.