Literature DB >> 21956560

Site-specific incorporation of unnatural amino acids as probes for protein conformational changes.

Jennifer C Peeler1, Ryan A Mehl.   

Abstract

Site-specific in vivo incorporation of unnatural amino acids provides powerful tools for the study of protein interaction and dynamics. Here, we provide a protocol for the incorporation of six such UAA probes into a GFP reporter system, expressed in Escherichia coli from both arabinose and lactose-inducible expression plasmids using an autoinduction media.

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Year:  2012        PMID： 21956560     DOI： 10.1007/978-1-61779-331-8_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Single-molecule resolution of protein structure and interfacial dynamics on biomaterial surfaces.

Authors:  Sean Yu McLoughlin; Mark Kastantin; Daniel K Schwartz; Joel L Kaar
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-14       Impact factor: 11.205

2.  Conformational Plasticity of the NNRTI-Binding Pocket in HIV-1 Reverse Transcriptase: A Fluorine Nuclear Magnetic Resonance Study.

Authors:  Naima G Sharaf; Rieko Ishima; Angela M Gronenborn
Journal:  Biochemistry       Date:  2016-07-11       Impact factor: 3.162

3.  Doping of Green Fluorescent Protein into Superfluid Helium Droplets: Size and Velocity of Doped Droplets.

Authors:  Maha Alghamdi; Jie Zhang; Andrew Oswalt; Joseph J Porter; Ryan A Mehl; Wei Kong
Journal:  J Phys Chem A       Date:  2017-08-31       Impact factor: 2.781

4.  Monitoring Replication Protein A (RPA) dynamics in homologous recombination through site-specific incorporation of non-canonical amino acids.

Authors:  Nilisha Pokhrel; Sofia Origanti; Eric Parker Davenport; Disha Gandhi; Kyle Kaniecki; Ryan A Mehl; Eric C Greene; Chris Dockendorff; Edwin Antony
Journal:  Nucleic Acids Res       Date:  2017-09-19       Impact factor: 16.971

5.  A Highly Versatile Expression System for the Production of Multiply Phosphorylated Proteins.

Authors:  Phillip Zhu; Philip R Gafken; Ryan A Mehl; Richard B Cooley
Journal:  ACS Chem Biol       Date:  2019-06-17       Impact factor: 5.100

Review 6.  Small, but powerful and attractive: 19F in biomolecular NMR.

Authors:  Angela M Gronenborn
Journal:  Structure       Date:  2021-12-13       Impact factor: 5.006

Review 7.  Incorporation of Non-Canonical Amino Acids.

Authors:  Lilia Leisle; Francis Valiyaveetil; Ryan A Mehl; Christopher A Ahern
Journal:  Adv Exp Med Biol       Date:  2015       Impact factor: 2.622

8.  The HIV-1 p66 homodimeric RT exhibits different conformations in the binding-competent and -incompetent NNRTI site.

Authors:  Naima G Sharaf; Zhaoyong Xi; Rieko Ishima; Angela M Gronenborn
Journal:  Proteins       Date:  2017-09-26

9.  Probing Protein-Protein Interactions with Genetically Encoded Photoactivatable Cross-Linkers.

Authors:  Richard B Cooley; Holger Sondermann
Journal:  Methods Mol Biol       Date:  2017

10.  Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP.

Authors:  Debashree Chatterjee; Richard B Cooley; Chelsea D Boyd; Ryan A Mehl; George A O'Toole; Holger Sondermann
Journal:  Elife       Date:  2014-09-02       Impact factor: 8.140
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