Literature DB >> 21953202

An efficient and inexpensive refrigerated LC system for H/D exchange mass spectrometry.

Theodore R Keppel1, Martin E Jacques, Robert W Young, Kenneth L Ratzlaff, David D Weis.   

Abstract

Loss of deuterium label during the LC step in amide hydrogen/deuterium exchange mass spectrometry (H/D-MS) is minimized by maintaining an acidic mobile phase pH and low temperature (pH 2.5, 0 °C). Here we detail the construction and performance of a low-cost, thermoelectrically refrigerated enclosure to house high-performance liquid chromatography (HPLC) components and cool mobile phases. Small volume heat exchangers rapidly decrease mobile phase temperature and keep the temperature stable to ±0.2 °C. Using a superficially porous reversed-phase column, we obtained excellent chromatographic performance in the separation of peptides with a median peak width of 4.4 s. Average deuterium recovery was 80.2% with an average relative precision of 0.91%.

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Year:  2011        PMID： 21953202     DOI： 10.1007/s13361-011-0152-6

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  14 in total

1.  Probing protein ligand interactions by automated hydrogen/deuterium exchange mass spectrometry.

Authors:  Michael J Chalmers; Scott A Busby; Bruce D Pascal; Yuanjun He; Christopher L Hendrickson; Alan G Marshall; Patrick R Griffin
Journal:  Anal Chem       Date:  2006-02-15       Impact factor: 6.986

2.  Influence of viscous friction heating on the efficiency of columns operated under very high pressures.

Authors:  Fabrice Gritti; Michel Martin; Georges Guiochon
Journal:  Anal Chem       Date:  2009-05-01       Impact factor: 6.986

3.  Assessment of the repeatability and reproducibility of hydrogen/deuterium exchange mass spectrometry measurements.

Authors:  William Burkitt; Gavin O'Connor
Journal:  Rapid Commun Mass Spectrom       Date:  2008-12       Impact factor: 2.419

4.  Isotope effects in peptide group hydrogen exchange.

Authors:  G P Connelly; Y Bai; M F Jeng; S W Englander
Journal:  Proteins       Date:  1993-09

5.  An experimental procedure for increasing the structural resolution of chemical hydrogen-exchange measurements on proteins: application to ribonuclease S peptide.

Authors:  J J Rosa; F M Richards
Journal:  J Mol Biol       Date:  1979-09-25       Impact factor: 5.469

6.  Aromatic ring-flipping in supercooled water: implications for NMR-based structural biology of proteins.

Authors:  J J Skalicky; J L Mills; S Sharma; T Szyperski
Journal:  J Am Chem Soc       Date:  2001-01-24       Impact factor: 15.419

7.  Deuterium exchange mass spectrometry as a probe of protein kinase activation. Analysis of wild-type and constitutively active mutants of MAP kinase kinase-1.

Authors:  K A Resing; N G Ahn
Journal:  Biochemistry       Date:  1998-01-13       Impact factor: 3.162

8.  Determination of pKa values of individual histidine residues in proteins using mass spectrometry.

Authors:  Masaru Miyagi; Takashi Nakazawa
Journal:  Anal Chem       Date:  2008-07-30       Impact factor: 6.986

9.  Primary structure effects on peptide group hydrogen exchange.

Authors:  Y Bai; J S Milne; L Mayne; S W Englander
Journal:  Proteins       Date:  1993-09

10.  Fast reversed-phase liquid chromatography to reduce back exchange and increase throughput in H/D exchange monitored by FT-ICR mass spectrometry.

Authors:  Hui-Min Zhang; George M Bou-Assaf; Mark R Emmett; Alan G Marshall
Journal:  J Am Soc Mass Spectrom       Date:  2008-11-21       Impact factor: 3.109
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  15 in total

1.  Prediction of the presence of a seventh ankyrin repeat in IκBε from homology modeling combined with hydrogen-deuterium exchange mass spectrometry (HDX-MS).

Authors:  Kristen M Ramsey; Dominic Narang; Elizabeth A Komives
Journal:  Protein Sci       Date:  2018-09       Impact factor: 6.725

2.  Minimizing carry-over in an online pepsin digestion system used for the H/D exchange mass spectrometric analysis of an IgG1 monoclonal antibody.

Authors:  Ranajoy Majumdar; Prakash Manikwar; John M Hickey; Jayant Arora; C Russell Middaugh; David B Volkin; David D Weis
Journal:  J Am Soc Mass Spectrom       Date:  2012-09-20       Impact factor: 3.109

3.  Insight to the interaction of the dihydrolipoamide acetyltransferase (E2) core with the peripheral components in the Escherichia coli pyruvate dehydrogenase complex via multifaceted structural approaches.

Authors:  Krishnamoorthy Chandrasekhar; Junjie Wang; Palaniappa Arjunan; Martin Sax; Yun-Hee Park; Natalia S Nemeria; Sowmini Kumaran; Jaeyoung Song; Frank Jordan; William Furey
Journal:  J Biol Chem       Date:  2013-04-11       Impact factor: 5.157

4.  Structural basis for activation of calcineurin by calmodulin.

Authors:  Julie Rumi-Masante; Farai I Rusinga; Terrence E Lester; Tori B Dunlap; Todd D Williams; A Keith Dunker; David D Weis; Trevor P Creamer
Journal:  J Mol Biol       Date:  2011-11-12       Impact factor: 5.469

5.  Effects of drying method and excipient on structure and stability of protein solids using solid-state hydrogen/deuterium exchange mass spectrometry (ssHDX-MS).

Authors:  Nathan E Wilson; Elizabeth M Topp; Qi Tony Zhou
Journal:  Int J Pharm       Date:  2019-06-25       Impact factor: 5.875

6.  Localized hydration in lyophilized myoglobin by hydrogen-deuterium exchange mass spectrometry. 1. Exchange mapping.

Authors:  Andreas M Sophocleous; Jun Zhang; Elizabeth M Topp
Journal:  Mol Pharm       Date:  2012-02-29       Impact factor: 4.939

7.  Mapping residual structure in intrinsically disordered proteins at residue resolution using millisecond hydrogen/deuterium exchange and residue averaging.

Authors:  Theodore R Keppel; David D Weis
Journal:  J Am Soc Mass Spectrom       Date:  2014-12-07       Impact factor: 3.109

8.  Minimizing back exchange in the hydrogen exchange-mass spectrometry experiment.

Authors:  Benjamin T Walters; Alec Ricciuti; Leland Mayne; S Walter Englander
Journal:  J Am Soc Mass Spectrom       Date:  2012-09-11       Impact factor: 3.109

9.  Isotope Labeling of Biomolecules: Structural Analysis of Viruses by HDX-MS.

Authors:  Miklos Guttman; Kelly K Lee
Journal:  Methods Enzymol       Date:  2015-07-04       Impact factor: 1.600

10.  Effects of drying method and excipient on the structure and physical stability of protein solids: Freeze drying vs. spray freeze drying.

Authors:  Tarun Tejasvi Mutukuri; Nathan E Wilson; Lynne S Taylor; Elizabeth M Topp; Qi Tony Zhou
Journal:  Int J Pharm       Date:  2020-12-15       Impact factor: 5.875
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