Literature DB >> 21951589

Protein trafficking rates assessed by quantum dot quenching with bromocresol green.

Cathleen D Valentine1, A S Verkman, Peter M Haggie.   

Abstract

Quantum dots are bright, photostable fluorophores used extensively to investigate biological processes. In this study, we report that bromocresol green (BCG) at low micromolar concentrations rapidly, efficiently and reversibly quenches the fluorescence of commercial quantum dots having a wide range of functionalities. The broad utility of BCG quenching of quantum dots in cell biology is showed in quantitative assays of trafficking of the β(2) -adrenergic receptor (β(2) AR) and the cystic fibrosis transmembrane conductance regulator (CFTR).
© 2011 John Wiley & Sons A/S.

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Year:  2011        PMID： 21951589      PMCID： PMC3237755          DOI： 10.1111/j.1600-0854.2011.01287.x

Source DB:  PubMed          Journal:  Traffic        ISSN： 1398-9219            Impact factor:   6.215


  10 in total

1.  Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin-induced intestinal fluid secretion.

Authors:  Tonghui Ma; Jay R Thiagarajah; Hong Yang; Nitin D Sonawane; Chiara Folli; Luis J V Galietta; A S Verkman
Journal:  J Clin Invest       Date:  2002-12       Impact factor: 14.808

Review 2.  Quantum dots for live cells, in vivo imaging, and diagnostics.

Authors:  X Michalet; F F Pinaud; L A Bentolila; J M Tsay; S Doose; J J Li; G Sundaresan; A M Wu; S S Gambhir; S Weiss
Journal:  Science       Date:  2005-01-28       Impact factor: 47.728

3.  Proteolytic activity monitored by fluorescence resonance energy transfer through quantum-dot-peptide conjugates.

Authors:  Igor L Medintz; Aaron R Clapp; Florence M Brunel; Theresa Tiefenbrunn; H Tetsuo Uyeda; Eddie L Chang; Jeffrey R Deschamps; Philip E Dawson; Hedi Mattoussi
Journal:  Nat Mater       Date:  2006-06-25       Impact factor: 43.841

Review 4.  Imaging single events at the cell membrane.

Authors:  Jyoti K Jaiswal; Sanford M Simon
Journal:  Nat Chem Biol       Date:  2007-02       Impact factor: 15.040

5.  Tracking of quantum dot-labeled CFTR shows near immobilization by C-terminal PDZ interactions.

Authors:  Peter M Haggie; Jung Kyung Kim; Gergely L Lukacs; A S Verkman
Journal:  Mol Biol Cell       Date:  2006-09-20       Impact factor: 4.138

6.  The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles.

Authors:  Qi Zhang; Yulong Li; Richard W Tsien
Journal:  Science       Date:  2009-02-12       Impact factor: 47.728

7.  One at a time, live tracking of NGF axonal transport using quantum dots.

Authors:  Bianxiao Cui; Chengbiao Wu; Liang Chen; Alfredo Ramirez; Elaine L Bearer; Wei-Ping Li; William C Mobley; Steven Chu
Journal:  Proc Natl Acad Sci U S A       Date:  2007-08-14       Impact factor: 11.205

8.  Confinement of β(1)- and β(2)-adrenergic receptors in the plasma membrane of cardiomyocyte-like H9c2 cells is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins but not caveolae.

Authors:  Cathleen D Valentine; Peter M Haggie
Journal:  Mol Biol Cell       Date:  2011-06-16       Impact factor: 4.138

9.  Reaching out for signals: filopodia sense EGF and respond by directed retrograde transport of activated receptors.

Authors:  Diane S Lidke; Keith A Lidke; Bernd Rieger; Thomas M Jovin; Donna J Arndt-Jovin
Journal:  J Cell Biol       Date:  2005-08-15       Impact factor: 10.539

10.  Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes.

Authors:  Manu Sharma; Francesca Pampinella; Csilla Nemes; Mohamed Benharouga; Jeffrey So; Kai Du; Kristi G Bache; Blake Papsin; Noa Zerangue; Harald Stenmark; Gergely L Lukacs
Journal:  J Cell Biol       Date:  2004-03-08       Impact factor: 10.539
  10 in total
  4 in total

1.  Evidence against cellular internalization in vivo of NMO-IgG, aquaporin-4, and excitatory amino acid transporter 2 in neuromyelitis optica.

Authors:  Julien Ratelade; Jeffrey L Bennett; A S Verkman
Journal:  J Biol Chem       Date:  2011-11-08       Impact factor: 5.157

Review 2.  Quantum dots for quantitative imaging: from single molecules to tissue.

Authors:  Tania Q Vu; Wai Yan Lam; Ellen W Hatch; Diane S Lidke
Journal:  Cell Tissue Res       Date:  2015-01-27       Impact factor: 5.249

3.  Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells.

Authors:  Eddie Y Chung; Christopher J Ochs; Yi Wang; Lei Lei; Qin Qin; Andrew M Smith; Alex Y Strongin; Roger Kamm; Ying-Xin Qi; Shaoying Lu; Yingxiao Wang
Journal:  Nano Lett       Date:  2015-07-27       Impact factor: 11.189

4.  Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state.

Authors:  Mohammad U Zahid; Liang Ma; Sung Jun Lim; Andrew M Smith
Journal:  Nat Commun       Date:  2018-05-08       Impact factor: 14.919
  4 in total

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