Literature DB >> 21947927

Simultaneous measurement of ¹H-¹⁵N and methyl ¹Hm-¹³Cm residual dipolar couplings in large proteins.

Xinli Liao1, Raquel Godoy-Ruiz, Chenyun Guo, Vitali Tugarinov.   

Abstract

A two-dimensional TROSY-based SIM-(13)C(m)-(1)H(m)/(1)H-(15)N NMR experiment for simultaneous measurements of methyl (1) D (CH) and backbone amide (1) D (NH) residual dipolar couplings (RDC) in {U-[(15)N,(2)H]; Ileδ1-[(13)CH(3)]; Leu,Val-[(13)CH(3)/(12)CD(3)]}-labeled samples of large proteins is described. Significant variation in the alignment tensor of the 82-kDa enzyme Malate synthase G is observed as a function of only slight changes in experimental conditions. The SIM-(13)C(m)-(1)H(m)/(1)H-(15)N data sets provide convenient means of establishing the alignment tensor characteristics via the measurement of (1) D (NH) RDCs in the same protein sample.

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Year:  2011        PMID： 21947927     DOI： 10.1007/s10858-011-9553-x

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  28 in total

1.  Evaluation of cross-correlation effects and measurement of one-bond couplings in proteins with short transverse relaxation times.

Authors:  G Kontaxis; G M Clore; A Bax
Journal:  J Magn Reson       Date:  2000-03       Impact factor: 2.229

2.  Characterization of molecular alignment in aqueous suspensions of Pf1 bacteriophage.

Authors:  M Zweckstetter; A Bax
Journal:  J Biomol NMR       Date:  2001-08       Impact factor: 2.835

3.  Prediction of charge-induced molecular alignment of biomolecules dissolved in dilute liquid-crystalline phases.

Authors:  Markus Zweckstetter; Gerhard Hummer; Ad Bax
Journal:  Biophys J       Date:  2004-06       Impact factor: 4.033

Review 4.  New techniques in structural NMR--anisotropic interactions.

Authors:  J H Prestegard
Journal:  Nat Struct Biol       Date:  1998-07

Review 5.  Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Authors:  M Ottiger; F Delaglio; A Bax
Journal:  J Magn Reson       Date:  1998-04       Impact factor: 2.229

6.  Quantitative 13C and 2H NMR relaxation studies of the 723-residue enzyme malate synthase G reveal a dynamic binding interface.

Authors:  Vitali Tugarinov; Lewis E Kay
Journal:  Biochemistry       Date:  2005-12-13       Impact factor: 3.162

7.  High resolution measurement of methyl 13C(m)-13C and 1H(m)-13C(m) residual dipolar couplings in large proteins.

Authors:  Chenyun Guo; Raquel Godoy-Ruiz; Vitali Tugarinov
Journal:  J Am Chem Soc       Date:  2010-10-13       Impact factor: 15.419

8.  Single Transition-to-single Transition Polarization Transfer (ST2-PT) in [15N,1H]-TROSY.

Authors:  K V Pervushin; G Wider; K Wüthrich
Journal:  J Biomol NMR       Date:  1998-08       Impact factor: 2.835

9.  Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution.

Authors:  David M Anstrom; Karen Kallio; S James Remington
Journal:  Protein Sci       Date:  2003-09       Impact factor: 6.725

10.  Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

Authors:  J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal:  Proc Natl Acad Sci U S A       Date:  1995-09-26       Impact factor: 11.205
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