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Literature DB >> 21940468

Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.

Mayank Patel¹, Rodrigo Gonzalez, Colin Halford, Michael A Lewinski, Elliot M Landaw, Bernard M Churchill, David A Haake.

Abstract

We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 2011 PMID： 21940468 PMCID： PMC3232961 DOI： 10.1128/JCM.01261-11

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

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