Literature DB >> 2193924

Molecular cloning and characterization of an extracellular protease gene from Aeromonas hydrophila.

O Rivero1, J Anguita, C Paniagua, G Naharro.   

Abstract

A structural gene which codes for an extracellular protease in Aeromonas hydrophilia SO2/2 and D13 was cloned in Escherichia coli C600-1 by using pBR322 as a vector. The gene codes for a temperature-stable protease with a molecular mass of approximately 38,000 daltons. The protein was secreted to the periplasm of E. coli C600-1 and purified by osmotic shock. Cloned protease (P3) was identical in molecular mass and properties to the one purified from A. hydrophila SO2/2 culture supernatant as an extracellular product.

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Year:  1990        PMID： 2193924      PMCID： PMC213372          DOI： 10.1128/jb.172.7.3905-3908.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

1.  Characterization of extracellular metallo- and serine-proteases of Aeromonas hydrophila strain B51.

Authors:  T P Nieto; A E Ellis
Journal:  J Gen Microbiol       Date:  1986-07

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Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

4.  Molecular cloning and expression in Escherichia coli of the structural gene for the hemolytic toxin aerolysin from Aeromonas hydrophila.

Authors:  S P Howard; J T Buckley
Journal:  Mol Gen Genet       Date:  1986-08

5.  Virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.

Authors:  J M Janda; L S Oshiro; S L Abbott; P S Duffey
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

6.  Marker exchange mutagenesis of the aerolysin determinant in Aeromonas hydrophila demonstrates the role of aerolysin in A. hydrophila-associated systemic infections.

Authors:  T Chakraborty; B Huhle; H Hof; H Bergbauer; W Goebel
Journal:  Infect Immun       Date:  1987-09       Impact factor: 3.441

7.  Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells.

Authors:  K Nagahari; T Tanaka; F Hishinuma; M Kuroda; K Sakaguchi
Journal:  Gene       Date:  1977-03       Impact factor: 3.688

8.  Aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea.

Authors:  F W Hickman-Brenner; K L MacDonald; A G Steigerwalt; G R Fanning; D J Brenner; J J Farmer
Journal:  J Clin Microbiol       Date:  1987-05       Impact factor: 5.948

9.  Amonabactin, a novel tryptophan- or phenylalanine-containing phenolate siderophore in Aeromonas hydrophila.

Authors:  S Barghouthi; R Young; M O Olson; J E Arceneaux; L W Clem; B R Byers
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

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Authors:  Y Santos; A E Toranzo; J L Barja; T P Nieto; T G Villa
Journal:  Infect Immun       Date:  1988-12       Impact factor: 3.441
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  16 in total

1.  Molecular Cloning of the Tryptophan Operon from an Aeromonas hydrophila Freshwater Isolate.

Authors:  J Anguita; O Rivero; G Naharro
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792

2.  Molecular and functional characterization of a ToxR-regulated lipoprotein from a clinical isolate of Aeromonas hydrophila.

Authors:  Lakshmi Pillai; Jian Sha; Tatiana E Erova; Amin A Fadl; Bijay K Khajanchi; Ashok K Chopra
Journal:  Infect Immun       Date:  2006-07       Impact factor: 3.441

3.  Cloning and expression of a chitinase gene from Aeromonas hydrophila in Escherichia coli.

Authors:  J P Chen; F Nagayama; M C Chang
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792

Review 4.  Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas.

Authors:  J M Janda
Journal:  Clin Microbiol Rev       Date:  1991-10       Impact factor: 26.132

Review 5.  Bacterial extracellular zinc-containing metalloproteases.

Authors:  C C Häse; R A Finkelstein
Journal:  Microbiol Rev       Date:  1993-12

6.  Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxotrophic aroA mutant as a live attenuated vaccine.

Authors:  C Hernanz Moral; E Flaño del Castillo; P López Fierro; A Villena Cortés; J Anguita Castillo; A Cascón Soriano; M Sánchez Salazar; B Razquín Peralta; G Naharro Carrasco
Journal:  Infect Immun       Date:  1998-05       Impact factor: 3.441

7.  Endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of Vibrio cholerae.

Authors:  T D Connell; D J Metzger; J Lynch; J P Folster
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

8.  Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate.

Authors:  J Anguita; L B Rodríguez Aparicio; G Naharro
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

9.  Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila.

Authors:  Giovanni Suarez; Johanna C Sierra; Jian Sha; Shaofei Wang; Tatiana E Erova; Amin A Fadl; Sheri M Foltz; Amy J Horneman; Ashok K Chopra
Journal:  Microb Pathog       Date:  2007-10-24       Impact factor: 3.738

10.  A lipase of Aeromonas hydrophila showing nonhemolytic phospholipase C activity.

Authors:  A B Ingham; J M Pemberton
Journal:  Curr Microbiol       Date:  1995-07       Impact factor: 2.188
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