PURPOSE: To design a method suitable for obtaining tissue samples from regions of different function as ascertained by magnetic resonance imaging (MRI). MATERIALS AND METHODS: In vivo MRI was used to create azimuthal projections of the heart from dilated cardiomyopathy transplant patients with the cardiac valves in the center and four concentric rings representing the septum and free wall. Tagged MRI could identify regions of different contractile strength that were then transferred onto the map projection. The resulting tissue sampling map was used to guide dissection of tissue samples from the explanted heart for analysis by electron microscopy (EM) as well as provide samples for subsequent mRNA analysis. Accuracy of the sampling was determined in a sheep heart using 17 fiduciary markers glued to the epicardial surface. RESULTS: Tagged MRI identified areas of "normal" (%S(c) -11), "poor" (%S(c) -4) and "failed" contraction (%S(c) +3). The mapping method we developed enabled straightforward sampling of these regions after surgical excision. EM showed good tissue preservation while the test of accuracy using the fiduciary markers showed a sampling accuracy of 0.3 ± 3.7 mm. This was similar to the resolution of tagged MRI images themselves. CONCLUSION: The methods we have developed can accurately guide tissue sampling for ex vivo tissue analysis.
PURPOSE: To design a method suitable for obtaining tissue samples from regions of different function as ascertained by magnetic resonance imaging (MRI). MATERIALS AND METHODS: In vivo MRI was used to create azimuthal projections of the heart from dilated cardiomyopathy transplant patients with the cardiac valves in the center and four concentric rings representing the septum and free wall. Tagged MRI could identify regions of different contractile strength that were then transferred onto the map projection. The resulting tissue sampling map was used to guide dissection of tissue samples from the explanted heart for analysis by electron microscopy (EM) as well as provide samples for subsequent mRNA analysis. Accuracy of the sampling was determined in a sheep heart using 17 fiduciary markers glued to the epicardial surface. RESULTS: Tagged MRI identified areas of "normal" (%S(c) -11), "poor" (%S(c) -4) and "failed" contraction (%S(c) +3). The mapping method we developed enabled straightforward sampling of these regions after surgical excision. EM showed good tissue preservation while the test of accuracy using the fiduciary markers showed a sampling accuracy of 0.3 ± 3.7 mm. This was similar to the resolution of tagged MRI images themselves. CONCLUSION: The methods we have developed can accurately guide tissue sampling for ex vivo tissue analysis.
Authors: David J Crossman; Alistair A Young; Peter N Ruygrok; Guy P Nason; David Baddelely; Christian Soeller; Mark B Cannell Journal: J Mol Cell Cardiol Date: 2015-05-04 Impact factor: 5.000
Authors: Xingyu Zhang; Brett R Cowan; David A Bluemke; J Paul Finn; Carissa G Fonseca; Alan H Kadish; Daniel C Lee; Joao A C Lima; Avan Suinesiaputra; Alistair A Young; Pau Medrano-Gracia Journal: PLoS One Date: 2014-10-31 Impact factor: 3.240
Authors: Pau Medrano-Gracia; Brett R Cowan; David A Bluemke; J Paul Finn; Alan H Kadish; Daniel C Lee; Joao A C Lima; Avan Suinesiaputra; Alistair A Young Journal: J Cardiovasc Magn Reson Date: 2013-09-13 Impact factor: 5.364
Authors: Pau Medrano-Gracia; Brett R Cowan; Bharath Ambale-Venkatesh; David A Bluemke; John Eng; John Paul Finn; Carissa G Fonseca; Joao A C Lima; Avan Suinesiaputra; Alistair A Young Journal: J Cardiovasc Magn Reson Date: 2014-07-30 Impact factor: 5.364