OBJECTIVE: To evaluate the relationship between duration of sexual abstinence and sperm selection on sperm DNA fragmentation (SDF). DESIGN: Prospective study based on normozoospermic individuals. SETTING: Fertility and IVF unit and university unit for research. PATIENT(S): Two cohorts of normozoospermic individuals: 21 men (aged 25-35 years) attending a clinic and with clearly adverse female factors; and a group of 12 selected donors (aged 20-25 years). INTERVENTION(S): SDF assessment using the sperm chromatin the dispersión test (Halosperm) in two cohorts of normozoospermic men. MAIN OUTCOME MEASURE(S): SDF assessment after 24 hours of abstinence with recurrent ejaculation (one every 24 hours) using neat sperm samples; and SDF assessment before and after sperm selection with abstinence of 3 hours. RESULT(S): Lower baseline levels of SDF were observed after shorter periods of abstinence between ejaculations (24 hours and 3 hours) than those recommended. This effect is much more marked after quick repetitive ejaculation (3 hours of abstinence) and sperm selection. CONCLUSION(S): The present results challenge the role of abstinence in current male infertility treatments and suggest that SDF can be efficiently reduced by a biological practice consisting of short-term recurrent ejaculation coupled with effective sperm selection.
OBJECTIVE: To evaluate the relationship between duration of sexual abstinence and sperm selection on sperm DNA fragmentation (SDF). DESIGN: Prospective study based on normozoospermic individuals. SETTING: Fertility and IVF unit and university unit for research. PATIENT(S): Two cohorts of normozoospermic individuals: 21 men (aged 25-35 years) attending a clinic and with clearly adverse female factors; and a group of 12 selected donors (aged 20-25 years). INTERVENTION(S): SDF assessment using the sperm chromatin the dispersión test (Halosperm) in two cohorts of normozoospermic men. MAIN OUTCOME MEASURE(S): SDF assessment after 24 hours of abstinence with recurrent ejaculation (one every 24 hours) using neat sperm samples; and SDF assessment before and after sperm selection with abstinence of 3 hours. RESULT(S): Lower baseline levels of SDF were observed after shorter periods of abstinence between ejaculations (24 hours and 3 hours) than those recommended. This effect is much more marked after quick repetitive ejaculation (3 hours of abstinence) and sperm selection. CONCLUSION(S): The present results challenge the role of abstinence in current male infertility treatments and suggest that SDF can be efficiently reduced by a biological practice consisting of short-term recurrent ejaculation coupled with effective sperm selection.
Authors: Brent M Hanson; Kenneth I Aston; Tim G Jenkins; Douglas T Carrell; James M Hotaling Journal: J Assist Reprod Genet Date: 2017-11-16 Impact factor: 3.412
Authors: Sandro C Esteves; Armand Zini; Robert Matthew Coward; Donald P Evenson; Jaime Gosálvez; Sheena E M Lewis; Rakesh Sharma; Peter Humaidan Journal: Andrologia Date: 2020-10-27 Impact factor: 2.775
Authors: B Jose Manuel Mayorga-Torres; Mauricio Camargo; Ashok Agarwal; Stefan S du Plessis; Ángela P Cadavid; Walter D Cardona Maya Journal: Reprod Biol Endocrinol Date: 2015-05-21 Impact factor: 5.211
Authors: Charles Welliver; Aaron D Benson; Luke Frederick; Benjamin Leader; Edna Tirado; Paul Feustel; James Kontio; Mary McAsey; Tobias S Köhler Journal: Transl Androl Urol Date: 2016-10