Literature DB >> 2192265

Induction of transformation and DNA synthesis after microinjection of raf proteins.

M R Smith1, G Heidecker, U R Rapp, H F Kung.   

Abstract

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3 cells induced DNA synthesis and morphological transformation, whereas microinjection of full-length c-raf had no effect. The amino terminus of the raf protein has an important negative regulatory influence; alteration of this region resulted in increased kinase activity and oncogenicity.

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Year:  1990        PMID： 2192265      PMCID： PMC360849          DOI： 10.1128/mcb.10.7.3828-3833.1990

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  26 in total

1.  Expression of raf oncogenes activates the PEA1 transcription factor motif.

Authors:  C Wasylyk; B Wasylyk; G Heidecker; M Huleihel; U R Rapp
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

2.  Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF beta-receptor.

Authors:  D K Morrison; D R Kaplan; J A Escobedo; U R Rapp; T M Roberts; L T Williams
Journal:  Cell       Date:  1989-08-25       Impact factor: 41.582

3.  S-phase induction and transformation of quiescent NIH 3T3 cells by microinjection of phospholipase C.

Authors:  M R Smith; S H Ryu; P G Suh; S G Rhee; H F Kung
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

4.  Identification and characterization of c-raf phosphoproteins in transformed murine cells.

Authors:  A M Schultz; T Copeland; S Oroszlan; U R Rapp
Journal:  Oncogene       Date:  1988-02       Impact factor: 9.867

5.  Expression of human c-raf-1 oncogene proteins in E. coli.

Authors:  W Kolch; T I Bonner; U R Rapp
Journal:  Biochem Biophys Res Commun       Date:  1988-05-16       Impact factor: 3.575

6.  raf family serine/threonine protein kinases in mitogen signal transduction.

Authors:  U R Rapp; G Heidecker; M Huleihel; J L Cleveland; W C Choi; T Pawson; J N Ihle; W B Anderson
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1988

7.  Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.

Authors:  D K Morrison; D R Kaplan; U Rapp; T M Roberts
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

8.  Mutational activation of c-raf-1 and definition of the minimal transforming sequence.

Authors:  G Heidecker; M Huleihel; J L Cleveland; W Kolch; T W Beck; P Lloyd; T Pawson; U R Rapp
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

9.  Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus.

Authors:  U R Rapp; M D Goldsborough; G E Mark; T I Bonner; J Groffen; F H Reynolds; J R Stephenson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

10.  New mammalian transforming retrovirus: demonstration of a polyprotein gene product.

Authors:  U R Rapp; F H Reynolds; J R Stephenson
Journal:  J Virol       Date:  1983-03       Impact factor: 5.103
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  13 in total

1.  High-intensity Raf signal causes cell cycle arrest mediated by p21Cip1.

Authors:  A Sewing; B Wiseman; A C Lloyd; H Land
Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

2.  95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site.

Authors:  R M Stephens; G Sithanandam; T D Copeland; D R Kaplan; U R Rapp; D K Morrison
Journal:  Mol Cell Biol       Date:  1992-09       Impact factor: 4.272

3.  Raf-induced proliferation or cell cycle arrest is determined by the level of Raf activity with arrest mediated by p21Cip1.

Authors:  D Woods; D Parry; H Cherwinski; E Bosch; E Lees; M McMahon
Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

4.  Raf-1 protein kinase activates the NF-kappa B transcription factor by dissociating the cytoplasmic NF-kappa B-I kappa B complex.

Authors:  S Li; J M Sedivy
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-15       Impact factor: 11.205

5.  Interleukin-2-triggered Raf-1 expression, phosphorylation, and associated kinase activity increase through G1 and S in CD3-stimulated primary human T cells.

Authors:  A Zmuidzinas; H J Mamon; T M Roberts; K A Smith
Journal:  Mol Cell Biol       Date:  1991-05       Impact factor: 4.272

6.  Induction of cell proliferation in quiescent NIH 3T3 cells by oncogenic c-Raf-1.

Authors:  E Kerkhoff; U R Rapp
Journal:  Mol Cell Biol       Date:  1997-05       Impact factor: 4.272

7.  Ligation of the T cell receptor complex results in activation of the Ras/Raf-1/MEK/MAPK cascade in human T lymphocytes.

Authors:  R A Franklin; A Tordai; H Patel; A M Gardner; G L Johnson; E W Gelfand
Journal:  J Clin Invest       Date:  1994-05       Impact factor: 14.808

8.  A Raf-1-related p110 polypeptide associates with the CD4-p56lck complex in T cells.

Authors:  K V Prasad; C E Rudd
Journal:  Mol Cell Biol       Date:  1992-11       Impact factor: 4.272

9.  Both p21ras and pp60v-src are required, but neither alone is sufficient, to activate the Raf-1 kinase.

Authors:  N G Williams; T M Roberts; P Li
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-01       Impact factor: 11.205

10.  Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor.

Authors:  H App; R Hazan; A Zilberstein; A Ullrich; J Schlessinger; U Rapp
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272
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