Craig Tipple1, Myra O McClure, Graham P Taylor. 1. Jefferiss Trust Laboratories, Wright-Fleming Institute, Imperial College London, Norfolk Place, London W2 1PG, UK. c.tipple@imperial.ac.uk
Abstract
OBJECTIVES: Macrolide resistant Treponema pallidum strains, caused by mutations in the 23S ribosomal RNA (23S rRNA) gene, are widespread and increasingly prevalent. The authors aimed to establish the strain types of T pallidum isolated from patients in a London sexual health centre and to determine the frequency of macrolide resistance. METHODS: T pallidum DNA from blood and ulcer samples were subjected to strain typing and mutation analysis using previously described methods. RESULTS: 18 samples were tested and a 23S rRNA point mutation conferring macrolide resistance was found in 66.6%. All resistant strains were collected from men who have sex with men and both the A2058G and the A2059G mutations were found. Two strain types were identified (14d/g and 14d/f); the predominant strain type was 14d/g and an association was noted between tp0548 type g and macrolide resistance. CONCLUSIONS: High levels of T pallidum macrolide resistance are present in London, UK, and this has clear implications for national treatment guidelines.
OBJECTIVES:Macrolide resistant Treponema pallidum strains, caused by mutations in the 23S ribosomal RNA (23S rRNA) gene, are widespread and increasingly prevalent. The authors aimed to establish the strain types of T pallidum isolated from patients in a London sexual health centre and to determine the frequency of macrolide resistance. METHODS: T pallidum DNA from blood and ulcer samples were subjected to strain typing and mutation analysis using previously described methods. RESULTS: 18 samples were tested and a 23S rRNA point mutation conferring macrolide resistance was found in 66.6%. All resistant strains were collected from men who have sex with men and both the A2058G and the A2059G mutations were found. Two strain types were identified (14d/g and 14d/f); the predominant strain type was 14d/g and an association was noted between tp0548 type g and macrolide resistance. CONCLUSIONS: High levels of T pallidummacrolide resistance are present in London, UK, and this has clear implications for national treatment guidelines.
Authors: Linda Grillová; Helena Pĕtrošová; Lenka Mikalová; Radim Strnadel; Eliška Dastychová; Ivana Kuklová; Martina Kojanová; Miluše Kreidlová; Daniela Vaňousová; Jana Hercogová; Přemysl Procházka; Hana Zákoucká; Alena Krchňáková; Vladimír Vašků; David Šmajs Journal: J Clin Microbiol Date: 2014-08-06 Impact factor: 5.948
Authors: Cheng-Yen Chen; Kai-Hua Chi; Allan Pillay; Eli Nachamkin; John R Su; Ronald C Ballard Journal: J Clin Microbiol Date: 2013-01-02 Impact factor: 5.948