Literature DB >> 21913076

Methylated-CpG Island Recovery Assay.

Natalie Mitchell1, J Tyson Deangelis, Trygve O Tollefsbol.   

Abstract

Alterations in DNA methylation patterns are implicated in playing a major role in the development of cancer, thus highlighting the need to continually develop new technologies to analyze epigenetic marks. Methylated-CpG Island Recovery Assay (MIRA), based on the high affinity of the MBD2b/MDB3L1 complex for double-stranded methylated DNA, allows for the recovery of methylated DNA without the use of bisulfite conversion or antibody recognition. MIRA is capable of detecting low-density methylation of a single methylated CpG nucleotide. This technique can be used in conjunction with microarrays or next-generation sequencing to analyze recovered methylated DNA on a genome-wide scale.

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Year:  2011        PMID： 21913076      PMCID： PMC3225287          DOI： 10.1007/978-1-61779-316-5_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  25 in total

Review 1.  Methyl CpG binding proteins: coupling chromatin architecture to gene regulation.

Authors:  P A Wade
Journal:  Oncogene       Date:  2001-05-28       Impact factor: 9.867

2.  Aberrant CpG-island methylation has non-random and tumour-type-specific patterns.

Authors:  J F Costello; M C Frühwald; D J Smiraglia; L J Rush; G P Robertson; X Gao; F A Wright; J D Feramisco; P Peltomäki; J C Lang; D E Schuller; L Yu; C D Bloomfield; M A Caligiuri; A Yates; R Nishikawa; H Su Huang; N J Petrelli; X Zhang; M S O'Dorisio; W A Held; W K Cavenee; C Plass
Journal:  Nat Genet       Date:  2000-02       Impact factor: 38.330

Review 3.  DNA methylation: a profile of methods and applications.

Authors:  Mario F Fraga; Manel Esteller
Journal:  Biotechniques       Date:  2002-09       Impact factor: 1.993

Review 4.  An overview of the analysis of DNA methylation in mammalian genomes.

Authors:  Masahiko Shiraishi; Adam J Oates; Takao Sekiya
Journal:  Biol Chem       Date:  2002-06       Impact factor: 3.915

5.  A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.

Authors:  M Frommer; L E McDonald; D S Millar; C M Collis; F Watt; G W Grigg; P L Molloy; C L Paul
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

Review 6.  CpG-rich islands and the function of DNA methylation.

Authors:  A P Bird
Journal:  Nature       Date:  1986 May 15-21       Impact factor: 49.962

Review 7.  Advantages of next-generation sequencing versus the microarray in epigenetic research.

Authors:  Paul J Hurd; Christopher J Nelson
Journal:  Brief Funct Genomic Proteomic       Date:  2009-06-17

8.  The affinity of different MBD proteins for a specific methylated locus depends on their intrinsic binding properties.

Authors:  Mario F Fraga; Esteban Ballestar; Guillermo Montoya; Panya Taysavang; Paul A Wade; Manel Esteller
Journal:  Nucleic Acids Res       Date:  2003-03-15       Impact factor: 16.971

Review 9.  The fundamental role of epigenetic events in cancer.

Authors:  Peter A Jones; Stephen B Baylin
Journal:  Nat Rev Genet       Date:  2002-06       Impact factor: 53.242

10.  Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II.

Authors:  J Singer; J Roberts-Ems; A D Riggs
Journal:  Science       Date:  1979-03-09       Impact factor: 47.728
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  5 in total

1.  A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer.

Authors:  Stella Tommasi; Ahmad Besaratinia
Journal:  Methods Mol Biol       Date:  2018

Review 2.  The Role of Epigenetic Change in Autism Spectrum Disorders.

Authors:  Yuk Jing Loke; Anthony John Hannan; Jeffrey Mark Craig
Journal:  Front Neurol       Date:  2015-05-26       Impact factor: 4.003

3.  Kaposi's sarcoma-associated herpesvirus genome programming during the early stages of primary infection of peripheral blood mononuclear cells.

Authors:  Hem C Jha; Jie Lu; Subhash C Verma; Shuvomoy Banerjee; Devan Mehta; Erle S Robertson
Journal:  mBio       Date:  2014-12-16       Impact factor: 7.867

Review 4.  DNA Methylation in Solid Tumors: Functions and Methods of Detection.

Authors:  Andrea Martisova; Jitka Holcakova; Nasim Izadi; Ravery Sebuyoya; Roman Hrstka; Martin Bartosik
Journal:  Int J Mol Sci       Date:  2021-04-19       Impact factor: 5.923

5.  Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.

Authors:  Mohammad Amin Kerachian; Marjan Azghandi; Sina Mozaffari-Jovin; Alain R Thierry
Journal:  Clin Epigenetics       Date:  2021-10-18       Impact factor: 6.551
  5 in total

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