Clinicopathological significance of BMP7 expression in esophageal squamous cell carcinoma.

BACKGROUND: Bone morphogenetic proteins (BMPs) are secreted signaling molecules belonging to the transforming growth factor-β (TGF-β) superfamily of growth factors. Recent studies have shown that the influence of the expression of BMP7 was altered in several tumors. The purpose of the current study was to examine the expression of BMP7 in esophageal squamous cell carcinoma and to clarify the clinical impact of BMP7 expression in esophageal squamous cell carcinoma (ESCC).
METHODS: A total of 180 patients with ESCC who underwent surgical resection from 1991 to 2004 were eligible for this study. The expression of BMP7 in esophageal tumor tissues was examined immunohistochemically.
RESULTS: BMP7 expression was found in the cytoplasm of cancer cells. BMP7 positivity was observed in 61.7% of tumors. The BMP7-positive group had deeper progression, more advanced stages, and greater venous invasion than those without BMP7 expression (p < 0.001, p < 0.005, and p < 0.0005, respectively). In addition, expression of BMP7 correlated with poorer prognosis (p < 0.0005). Multivariate analysis showed that BMP7 expression status was an independent prognostic factor (p < 0.05).
CONCLUSIONS: Patients with expression of BMP7 in ESCC had high malignant potential. BMP7 could be a useful prognostic marker for patients with ESCC.

Esophageal squamous cell carcinoma (ESCC) is aggressive in gastrointestinal tract carcinomas. Even when patients with ESCC undergo curative resection, postoperative relapse often occurs. Attempts to evaluate the group at high risk of recurrence have been made in respect to tumor aggressiveness. The tumor behavior of ESCC at the molecular level has been intensively analyzed.

Among the molecular factors available for possible evaluation of tumor aggressiveness, bone morphogenetic protein (BMP) is a member of the transforming growth factor (TGF)-β superfamily known to regulate cell proliferation, apoptosis, and differentiation during human development.1,2 BMPs bind cell membrane type I and type II receptors of serine/threonine kinases, eliciting intracellular signaling via Smad1, 5, 8, and 9 proteins.3,4 Recent studies have shown that BMP7 maintains epithelial and endothelial phenotypes against epithelial-mesenchymal transition (EMT).5,6

BMP7 is present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation.7–9 Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival.10 However, the expression of BMP7 in ESCC has not been evaluated.

In the present study, the BMP7 expression in surgical specimens of ESCC was examined to evaluate whether this molecule is useful to predict postoperative outcome.

Materials and Methods

Patients and Specimens

There were 180 patients diagnosed with ESCC (163 males and 17 females) who underwent R0 esophagectomy with lymph node dissection between 1991 and 2004 at Kagoshima University Hospital, Kagoshima, Japan. The median age of the patients was 65.2 (range, 39–84) years.

None of these patients underwent endoscopic mucosal resection, preoperative chemotherapy or radiotherapy, or had metachronous multiple cancer in other organs. Specimens of cancer tissue and noncancerous adjacent tissue were collected from patients after informed consent had been obtained in accordance with the institutional guidelines of our hospital. Using TNM classification of the International Union Against Cancer,11 all of the M1 tumors exhibited distant lymph node metastases. All patients were followed-up after discharge, with x-ray examination and tumor marker assays (squamous cell carcinoma antigen and carcinoembryonic antigen) performed every 1 to 3 months, computed tomography every 3 to 6 months, and ultrasonography every 6 months. Bronchoscopy and endoscopy were performed if necessary.

Postoperative follow-up data were obtained from all patients, with a median follow-up period of 39 (range, 1–137) months. According to TNM classification, 74 of the 180 patients had T1 tumors, 25 patients had T2 tumors, 78 patients had T3 tumors, and 3 patients had T4 tumors.

Immunohistochemical Staining and Evaluation of BMP7 in ESCC

Tumor samples were fixed with 10% formaldehyde in phosphate-buffered saline (PBS), embedded in paraffin, and sectioned into 4-μm-thick slices. They were deparaffinized in xylene and dehydrated with a series of graded ethanol. The endogenous peroxidase activity of specimens was blocked by immersing the slides in a 3% hydrogen peroxidase-methanol solution for 10 min at room temperature. After washing three times with PBS for 5 min each, the sections were treated with 1% bovine serum albumin for 30 min to block nonspecific reactions at room temperature. Then, the sections were incubated with primary antibody to BMP7 (1:200 R&D System) at 4°C overnight and subsequently stained with secondary antibodies for 30 min. The sections were incubated with avidin–biotin complex for 60 min, and reactions were visualized using diaminobenzidine tetrahydrochloride for 2 min. The sections were rinsed briefly in water and counterstained with hematoxylin for 30 s. Normal human kidney was used as a positive (medullary rays) and negative (glomeruli) control to verify both BMP7 antibody specificity and staining quality (Fig. 1a).12 Immunohistochemistry was independently evaluated by two investigators (KM and SI) who had no information about the patients’ clinical data. In cases in which the immunohistochemical evaluation of the two observers differed, slides were evaluated by a third observer (SN).

Fig. 1

Normal kidney section as a positive control for BMP7 staining. a BMP7 expression was seen in medullary rays and not seen in glomeruli (×200). Expression of BMP7 in ESCCs. b Negative expression of BMP7 (×200). c Positive expression of BMP7 was detectable in cytoplasm and membranes (×200). Scale bar 100 μm

To evaluate the expression of BMP7, ten fields (within the tumor and at the invasive front) were selected and expression in 1,000 tumor cells (100 cells/field) was evaluated using high-power (×200) microscopy. Expression of BMP7 was assessed using the proportion of positive cells and using intensity. At first, we classified each sample into one of three categories in terms of the proportion of positive cells: 1+, 0–29%; 2+, 30–59%; and 3+, ≥60% positive cells. In addition, we classified each sample into one of three categories according to the intensity: 1+, weak; 2+, moderate; and 3+, strong. The tumors with strong staining at least 30% of the cells and moderate staining at least 60% of the cells were considered BMP7-positive. We used this evaluation because of the heterogeneity of the ESCC samples and the fact that the deeper the tumors invaded, the stronger their intensity tended to become. We improved the evaluation method used by Liu et al.13

Statistical Analysis

Statistical analysis of group differences was performed using the χ2 test. The Kaplan-Meier method was used for survival analysis, and differences in survival were estimated by using the log-rank test. Prognostic factors were examined by univariate and multivariate analyses (Cox proportional hazards regression model). A p value <0.05 was considered to indicate statistical significance. All statistical analyses were performed using the StatView for Windows software (Version 5.0, Abacus Concepts, Berkeley, CA).

Results

BMP7 Expression of ESCC

BMP7 expression was identified in cellular membrane and cytoplasm of ESCC and was detected rarely in normal esophageal epithelium. Positivity of BMP7 expression was seen in 111 (61.7%) of 180 patients (Fig. 1c).

Relationship Between BMP7 Expression and Clinicopathological Features

High-intensity BMP7 expression was detected more at deeper areas in ESCC. The correlations between BMP7 expression and clinicopathological characteristics are shown in Table 1. The BMP7-positive group had significantly deeper tumor invasion (p < 0.001), more advanced stages (p = 0.0012), more venous invasion (p = 0.0002), and more locoregional recurrence (p = 0.027) than the BMP7-negative group. We had 23 locoregional recurrences during the period of this study: 3 were surgical anastomosis recurrences, and 20 were lymph node recurrences. Furthermore, we examined the relationship between BMP7 expression and locoregional recurrence; 19 of 23 patients with locoregional recurrence had BMP7-positive expression (Table 2).

Table 1

Relationship between BMP7 expression and clinicopathologic findings

	BMP7 expression		p
	(−) n = 69	(+) n = 111
Total (N = 180)
Age (mean ± SD)	64.6 ± 9	65.6 ± 9	0.975
Gender
Male	65	98	0.178
Female	4	13
Histology
Well	19	36	0.588
Moderate	34	53
Poor	9	22
pT
T1	36	23	<0.0001
T2, T3, T4	33	88
pN
N0	34	43	0.171
N1	35	68
pM
M0	55	78	0.06
M1	14	33
Lymphatic invasion
Negative	24	24	0.052
Positive	45	87
Venous invasion
Negative	40	33	0.0002
Positive	29	78
Stage
I	20	13	0.0012
IIA, IIB	25	28
III	10	37
IV	14	33
Locoregional recurrence
Negative	65	4	0.027
Positive	92	19

SD standard deviation

Table 2

Relationship between immunohistochemical staining for BMP7 expression and locoregional recurrence

Recurrence site	Total	BMP7 expression
		Positive (n = 19)	Negative (n = 4)
Lymph nodes	20	17 (85%)	3 (15%)
Surgical anastomosis	3	2 (66%)	1 (33%)

BMP7 Expression and Patients’ Survival

The 5-year overall survival rate was significantly lower in patients with BMP7-positive expression than in those with negative expression (p = 0.0004; Fig. 2a). In 77 patients who had no lymph node metastasis, 43 patients with BMP7-positive expression also had significantly lower 5-year overall survival rate than that of patients judged to have BMP7-negative expression (p = 0.0016; Fig. 2b).

Fig. 2

a Overall 5-year survival curves of patients with esophageal cancer according to the expression of BMP7. Patients who were positive for BMP7 expression exhibited significantly poorer prognosis than those negative for BMP7 expression. b Overall 5-year survival curves of patients without lymph node metastasis in relation to BMP7 expression. Patients who were positive for BMP7 expression exhibited significantly poorer prognosis than those negative for BMP7 expression

Univariate and Multivariate Analysis of Survival

Table 3 shows the results of univariate and multivariate analyses of the factors related to patient prognosis. Univariate regression analyses revealed that depth of tumor invasion, lymph node metastasis, pM status (distant lymph node metastasis), venous invasion, locoregional recurrence, and BMP7 expression significantly affected postoperative outcome. Multivariate analysis indicated that BMP7 expression was one of the independent prognostic factors (p = 0.046), along with the depth of invasion (p = 0.019), and pM status (distant lymph node metastasis) (p = 0.009; Table 3).

Table 3

Univariate and multivariate analysis of prognostic factors in ESCC

Factors	Univariate analysis p	Multivariate analysis
		p	Hazard ratio	95% CI
pT (1/2,3,4) (negative/positive)	<0.0001*	0.019*	1.89	1.107–3.225
pN (negative/positive)	0.0003*	0.377	1.269	0.748–2.15
Venous invasion (negative/positive)	0.006*	0.622	1.121	0.71–1.769
pM (negative/positive)	<0.0001*	0.009*	1.93	1.177–3.164
Locoregional recurrence (negative/positive)	0.03*	0.587	1.162	0.673–2.008
BMP7 expression	0.0004*	0.046*	1.61	1.008–2.576

* p < 0.05

Discussion

This is the first report to show an association between BMP7 expression and clinicopathological findings in ESCC. BMP7 positivity was observed in 61.7% of ESCC tumors, which was a higher incidence than in colorectal (41%) and breast cancers (46%).10,14

BMP7 expression have been reported to be involved in the growth of several cancer cells, such as osteosarcoma, malignant melanoma, prostate cancer, breast cancer, renal cell cancer, colorectal cancer, and gastric cancer, causing increased aggression or suppression.10,15–20 We showed that BMP7 expression in ESCC was significantly associated with the tumor depth, stages, and venous invasion. Motoyama et al. investigated BMP7 mRNA in colorectal cancer and showed its significant association with tumor extension and liver metastasis.10 Aoki et al. reported immunohistochemical expression of BMP7 in gastric cancer was significantly related to the depth of tumors, nodal involvement, lymphatic invasion, and venous invasion.20 Those findings agree with our results. In this context, cancerous BMP7 positivity may reflect tumor aggressiveness.

In contrast, Kwak et al. reported that cancerous BMP7 expression correlated with better surgical outcome in renal cell carcinoma.15 Renal cells are used as a positive control of BMP7 expression; this contradictory finding supports organ-specificity. Aoki et al. also reported that BMP7 expression in gastric cancer was significantly higher in the well- and moderately differentiated histological group than in the poorly differentiated histological group. In contrast, in this study, we showed no relationship between BMP7 expression and tumor histological type; this contradictory findings supports tumor-specificity (histologic differences between squamous cell carcinoma and adenocarcinoma).

BMP7 expression by immunohistochemistry was significantly associated with lower recurrence-free survival in malignant melanoma and breast cancer.14,17 Our recent study showed BMP7 expression was significantly related to the locoregional recurrence. Those who had BMP7 expression tended to occur local lymph node relapse. Those findings might have important clinical significance. To determinate BMP7 expression status in primary ESCC could help to identify patients who are at high risk to develop early local lymph node recurrence.

In vitro, the BMP7 signaling pathway in malignant tumors has not been fully understood. From our results, in ESCC, these specific signals for BMP7 may correspond with tumor invasion in those signals that BMP7 stimulates.

In many cancers, TGF-β is a protumorigenic factor that stimulates EMT.21–23 In contrast, BMP7 is a strong inducer of the reverse process (mesenchymal-epithelial transition; MET) during embryonic development.24–26 Moreover, BMP7 can inhibit TGF-β-induced fibrosis and counteracts TGF-β-induced EMT in normal renal epithelial cells.27–29 In contrast, in this study, overexpression of BMP7 in ESCC was significantly related to tumor aggressiveness. BMP7 might be related to tumor migration ability in ESCC. We need to find out more details about the cross-talk between BMP7 and TGF-β signaling pathways in the context of EMT.

As a prognostic parameter, Aoki et al. reported that immunohistochemical BMP7 expression in gastric cancer patients was associated with poor overall survival.20 Moreover, overexpression of BMP7 mRNA was significantly associated with lower overall survival in colorectal cancer.10

In this study, concerning the overall survival analysis, tumor depth, lymph node metastasis, venous invasion, distant lymph node metastasis, locoregional recurrence, and BMP7 expression were prognostic factors on univariate analysis. We showed that BMP7 expression was one of independent prognostic factors, along with tumor depth, and distant lymph node metastasis on multivariate analysis in accordance with results for other digestive tract cancers. BMP7 expression could be used as a useful prognostic parameter predicting the survival of postoperative ESCC patients.

Various molecular markers have been shown to be associated with development and progression of ESCC, including cyclin D1, smad4, Fas, cyclin B1, CD151, Bmi-1, and osteopontin.13,30–35 Naitoh et al. demonstrated that the survival rate for the patients with overexpression of cyclin D1 was very low even in the patients without lymph node metastasis.30 In this study, the 5-year overall survival rate for the patients with BMP7 positive expression was significantly worse than those with negative expression even in the patients without lymph node metastasis. BMP7 expression may be a useful marker for high-risk patients of ESCC without lymph node metastasis. This enables us to choose postoperative treatment, including chemotherapy.

Moreover, we showed that BMP7 expression tends to correlate with lymphatic invasion (p = 0.052). Thus, cancerous BMP7 expression in a biopsy may be informative to predict lymphatic invasion. When selecting whether to perform endoscopic resection for early ESCC, we can use cancerous BMP7 expression as a predictor of lymphatic invasion.

In conclusion, BMP7 expression in ESCC plays a critical role in tumor proliferation and invasion. Expression of BMP7 may be a predictor of poor overall survival. This independent molecular marker for high-risk patients could help us in our attempts to individualize patients’ therapy.