Literature DB >> 21898255

Isolation of genetic material from Arabidopsis seeds.

Urszula Piskurewicz1, Luis Lopez-Molina.   

Abstract

Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

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Year:  2011        PMID: 21898255     DOI: 10.1007/978-1-61779-231-1_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

1.  Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Genomic DNA from Mature Seed Tissues.

Authors:  Mayumi Iwasaki; Luis Lopez-Molina
Journal:  Methods Mol Biol       Date:  2021

2.  Dormancy-specific imprinting underlies maternal inheritance of seed dormancy in Arabidopsis thaliana.

Authors:  Urszula Piskurewicz; Mayumi Iwasaki; Daichi Susaki; Christian Megies; Tetsu Kinoshita; Luis Lopez-Molina
Journal:  Elife       Date:  2016-12-22       Impact factor: 8.140

3.  Dynamic DNA methylation reconfiguration during seed development and germination.

Authors:  Taiji Kawakatsu; Joseph R Nery; Rosa Castanon; Joseph R Ecker
Journal:  Genome Biol       Date:  2017-09-15       Impact factor: 13.583

4.  Non-canonical RNA-directed DNA methylation participates in maternal and environmental control of seed dormancy.

Authors:  Mayumi Iwasaki; Lena Hyvärinen; Urszula Piskurewicz; Luis Lopez-Molina
Journal:  Elife       Date:  2019-03-26       Impact factor: 8.140

5.  The phytochrome interacting proteins ERF55 and ERF58 repress light-induced seed germination in Arabidopsis thaliana.

Authors:  Zenglin Li; David J Sheerin; Edda von Roepenack-Lahaye; Mark Stahl; Andreas Hiltbrunner
Journal:  Nat Commun       Date:  2022-03-29       Impact factor: 14.919

  5 in total

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