| Literature DB >> 21887654 |
Yuanyuan Chen1, Bernard A Prior, Guiyang Shi, Zhengxiang Wang.
Abstract
In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.Entities:
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Year: 2011 PMID： 21887654 DOI： 10.1007/s12275-011-0525-3
Source DB: PubMed Journal: J Microbiol ISSN： 1225-8873 Impact factor: 3.422