Literature DB >> 14967235

A rapid DNA extraction method for PCR identification of fungal indoor air contaminants.

Timothy R Dean1, Doris Betancourt, Marc Y Menetrez.   

Abstract

Following air sampling fungal DNA needs to be extracted and purified to a state suitable for laboratory use. Our laboratory has developed a simple method of extraction and purification of fungal DNA appropriate for enzymatic manipulation and Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective for use with multiple fungal organisms.

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Year:  2004        PMID： 14967235     DOI： 10.1016/j.mimet.2003.11.015

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  9 in total

1.  A simple polymerase chain reaction/restriction fragment length polymorphism assay capable of identifying medically relevant filamentous fungi.

Authors:  Timothy R Dean; Michael Kohan; Doris Betancourt; Marc Y Menetrez
Journal:  Mol Biotechnol       Date:  2005-09       Impact factor: 2.695

2.  A simple polymerase chain reaction-sequencing analysis capable of identifying multiple medically relevant filamentous fungal species.

Authors:  Timothy R Dean; Michael Kohan; Doris Betancourt; Marc Y Menetrez
Journal:  Mycopathologia       Date:  2006-10       Impact factor: 2.574

3.  A rapid PCR-based approach for molecular identification of filamentous fungi.

Authors:  Yuanyuan Chen; Bernard A Prior; Guiyang Shi; Zhengxiang Wang
Journal:  J Microbiol       Date:  2011-09-02       Impact factor: 3.422

4.  Biotransformation of 2,4-dinitroanisole by a fungal Penicillium sp.

Authors:  Hunter W Schroer; Kathryn L Langenfeld; Xueshu Li; Hans-Joachim Lehmler; Craig L Just
Journal:  Biodegradation       Date:  2016-12-02       Impact factor: 3.909

5.  Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Authors:  X Libert; C Chasseur; S Bladt; A Packeu; F Bureau; N H Roosens; S C J De Keersmaecker
Journal:  Appl Microbiol Biotechnol       Date:  2015-07-17       Impact factor: 4.813

6.  Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

Authors:  Xavier Libert; Ann Packeu; Fabrice Bureau; Nancy H Roosens; Sigrid C J De Keersmaecker
Journal:  PLoS One       Date:  2017-03-09       Impact factor: 3.240

7.  Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review.

Authors:  Jordan Peccia; Mark Hernandez
Journal:  Atmos Environ (1994)       Date:  2006-04-18       Impact factor: 4.798

8.  Microbial volatile organic compound emissions from Stachybotrys chartarum growing on gypsum wallboard and ceiling tile.

Authors:  Doris A Betancourt; Ken Krebs; Scott A Moore; Shayna M Martin
Journal:  BMC Microbiol       Date:  2013-12-05       Impact factor: 3.605

9.  Exploiting the Advantages of Molecular Tools for the Monitoring of Fungal Indoor Air Contamination: First Detection of Exophiala jeanselmei in Indoor Air of Air-Conditioned Offices.

Authors:  Xavier Libert; Camille Chasseur; Ann Packeu; Fabrice Bureau; Nancy H Roosens; Sigrid C J De Keersmaecker
Journal:  Microorganisms       Date:  2019-12-10
  9 in total

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