PURPOSE: Transcorneal electrical stimulation (TES) has been beneficial in several neurodegenerative ocular diseases, but the exact mechanisms remain to be elucidated. This study was conducted to investigate the effects of TES on the retinas of wild-type Brown Norway (BN) rats by gene expression profiling and to assess its effects on retinal function and morphology. METHODS: TES was applied to BN wild-type rat retinas in vivo for 1 hour (1-ms biphasic pulses at 20 Hz; 200 μA). RNA was isolated and processed for microarray-based profiling 4 hours after TES; differentially expressed genes from TES compared with those from sham-treated animals were validated by quantitative real-time polymerase chain reaction. Furthermore, the effect of TES was assessed at the structural and functional levels using electroretinography, confocal scanning laser ophthalmoscopy, optical coherence tomography, and immunohistochemistry. RESULTS: Transcriptome changes associated with TES versus sham-stimulated BN wild-type retina were identified. Four hundred ninety genes were differentially expressed in TES and included potentially neuroprotective genes such as Bax or members of the tumor necrosis factor family (Tnfrsf11b, Tnrsf12a, Tnfsf13b, Tnfsf13). ERG recordings showed physiological retinal function after TES, and structural in vivo and ex vivo studies revealed intact retinal anatomy. CONCLUSIONS: These results demonstrate that TES applied to the retina of the wild-type BN rats induces distinct transcriptome level changes and may help in the understanding of the mechanisms underlying TES. In addition, TES treatment indicates no negative effect on structure and function of the wild-type BN retina up to 35 hours after application.
PURPOSE: Transcorneal electrical stimulation (TES) has been beneficial in several neurodegenerative ocular diseases, but the exact mechanisms remain to be elucidated. This study was conducted to investigate the effects of TES on the retinas of wild-type Brown Norway (BN) rats by gene expression profiling and to assess its effects on retinal function and morphology. METHODS:TES was applied to BN wild-type rat retinas in vivo for 1 hour (1-ms biphasic pulses at 20 Hz; 200 μA). RNA was isolated and processed for microarray-based profiling 4 hours after TES; differentially expressed genes from TES compared with those from sham-treated animals were validated by quantitative real-time polymerase chain reaction. Furthermore, the effect of TES was assessed at the structural and functional levels using electroretinography, confocal scanning laser ophthalmoscopy, optical coherence tomography, and immunohistochemistry. RESULTS: Transcriptome changes associated with TES versus sham-stimulated BN wild-type retina were identified. Four hundred ninety genes were differentially expressed in TES and included potentially neuroprotective genes such as Bax or members of the tumor necrosis factor family (Tnfrsf11b, Tnrsf12a, Tnfsf13b, Tnfsf13). ERG recordings showed physiological retinal function after TES, and structural in vivo and ex vivo studies revealed intact retinal anatomy. CONCLUSIONS: These results demonstrate that TES applied to the retina of the wild-type BN rats induces distinct transcriptome level changes and may help in the understanding of the mechanisms underlying TES. In addition, TES treatment indicates no negative effect on structure and function of the wild-type BN retina up to 35 hours after application.
Authors: Abby Leigh Manthey; Wei Liu; Zhi Xin Jiang; Marcus Hiu Kong Lee; Jian Ji; Kwok-Fai So; Jimmy Shiu Ming Lai; Vincent Wing Hong Lee; Kin Chiu Journal: Cell Transplant Date: 2017-02-03 Impact factor: 4.064
Authors: Ninel Z Gregori; Natalia F Callaway; Catherine Hoeppner; Alex Yuan; Aleksandra Rachitskaya; William Feuer; Hossein Ameri; J Fernando Arevalo; Albert J Augustin; David G Birch; Gislin Dagnelie; Salvatore Grisanti; Janet L Davis; Paul Hahn; James T Handa; Allen C Ho; Suber S Huang; Mark S Humayun; Raymond Iezzi; K Thiran Jayasundera; Gregg T Kokame; Byron L Lam; Jennifer I Lim; Naresh Mandava; Sandra R Montezuma; Lisa Olmos de Koo; Peter Szurman; Lejla Vajzovic; Peter Wiedemann; James Weiland; Jiong Yan; David N Zacks Journal: Am J Ophthalmol Date: 2018-06-27 Impact factor: 5.258
Authors: Adam M Hanif; Moon K Kim; Joel G Thomas; Vincent T Ciavatta; Micah Chrenek; John R Hetling; Machelle T Pardue Journal: Exp Eye Res Date: 2016-06-18 Impact factor: 3.467
Authors: T Röck; A Schatz; L Naycheva; M Gosheva; J Pach; B Wilhelm; T Peters; K U Bartz-Schmidt; E Zrenner; G Willmann; F Gekeler Journal: Ophthalmologe Date: 2013-01 Impact factor: 1.059
Authors: T Röck; L Naycheva; G Willmann; B Wilhelm; T Peters; E Zrenner; K U Bartz-Schmidt; F Gekeler; A Schatz Journal: Ophthalmologe Date: 2017-10 Impact factor: 1.059