Dalia Mukhtar-Fayyad1. 1. Faculty of Oral and Dental Medicine, Misr International University, Cairo, Egypt. daliafayyad222@yahoo.com
Abstract
OBJECTIVE: The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells. STUDY DESIGN: Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test. RESULTS: A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods (P < .001). The cytotoxic effect of both materials was concentration dependant. CONCLUSIONS: Both BioAggregate and iRoot SP displayed an acceptable biocompatibility.
OBJECTIVE: The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells. STUDY DESIGN: Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test. RESULTS: A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods (P < .001). The cytotoxic effect of both materials was concentration dependant. CONCLUSIONS: Both BioAggregate and iRoot SP displayed an acceptable biocompatibility.