Literature DB >> 21870263

Purification of human PARP-1 and PARP-1 domains from Escherichia coli for structural and biochemical analysis.

Marie-France Langelier1, Jamie L Planck, Kristin M Servent, John M Pascal.   

Abstract

A general method to express and purify full-length human poly(ADP-ribose) polymerase-1 (PARP-1), individual PARP-1 domains, and groups of PARP-1 domains from Escherichia coli cells is described. The procedure allows for robust production of highly pure PARP-1 that is free of DNA contamination and well-suited for biochemical experiments and for structural and biophysical analysis. Two biochemical assays for monitoring PARP-1 automodification activity are presented that can be used to evaluate purified PARP-1, combinations of PARP-1 domains, or PARP-1 mutants.

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Year:  2011        PMID： 21870263     DOI： 10.1007/978-1-61779-270-0_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  40 in total

1.  Structural Basis for Potency and Promiscuity in Poly(ADP-ribose) Polymerase (PARP) and Tankyrase Inhibitors.

Authors:  Ann-Gerd Thorsell; Torun Ekblad; Tobias Karlberg; Mirjam Löw; Ana Filipa Pinto; Lionel Trésaugues; Martin Moche; Michael S Cohen; Herwig Schüler
Journal:  J Med Chem       Date:  2016-12-21       Impact factor: 7.446

2.  ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method.

Authors:  Casey M Daniels; Shao-En Ong; Anthony K L Leung
Journal:  Methods Mol Biol       Date:  2017

3.  Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin.

Authors:  Maggie H Chassé; Uma M Muthurajan; Nicholas J Clark; Michael A Kramer; Srinivas Chakravarthy; Thomas Irving; Karolin Luger
Journal:  Methods Mol Biol       Date:  2017

4.  PARP-1 Activation Requires Local Unfolding of an Autoinhibitory Domain.

Authors:  Jennine M Dawicki-McKenna; Marie-France Langelier; Jamie E DeNizio; Amanda A Riccio; Connie D Cao; Kelly R Karch; Michael McCauley; Jamin D Steffen; Ben E Black; John M Pascal
Journal:  Mol Cell       Date:  2015-11-25       Impact factor: 17.970

5.  Structure and mechanism of a canonical poly(ADP-ribose) glycohydrolase.

Authors:  Mark S Dunstan; Eva Barkauskaite; Pierre Lafite; Claire E Knezevic; Amy Brassington; Marijan Ahel; Paul J Hergenrother; David Leys; Ivan Ahel
Journal:  Nat Commun       Date:  2012-06-06       Impact factor: 14.919

6.  The BRCT domain of PARP1 binds intact DNA and mediates intrastrand transfer.

Authors:  Johannes Rudolph; Uma M Muthurajan; Megan Palacio; Jyothi Mahadevan; Genevieve Roberts; Annette H Erbse; Pamela N Dyer; Karolin Luger
Journal:  Mol Cell       Date:  2021-12-16       Impact factor: 17.970

7.  Captured snapshots of PARP1 in the active state reveal the mechanics of PARP1 allostery.

Authors:  Élise Rouleau-Turcotte; Dragomir B Krastev; Stephen J Pettitt; Christopher J Lord; John M Pascal
Journal:  Mol Cell       Date:  2022-07-05       Impact factor: 19.328

8.  Targeting PARP-1 allosteric regulation offers therapeutic potential against cancer.

Authors:  Jamin D Steffen; Renee M Tholey; Marie-France Langelier; Jamie L Planck; Matthew J Schiewer; Shruti Lal; Nikolai A Bildzukewicz; Charles J Yeo; Karen E Knudsen; Jonathan R Brody; John M Pascal
Journal:  Cancer Res       Date:  2013-11-04       Impact factor: 12.701

9.  Dynamics of the HD regulatory subdomain of PARP-1; substrate access and allostery in PARP activation and inhibition.

Authors:  Tom E H Ogden; Ji-Chun Yang; Marianne Schimpl; Laura E Easton; Elizabeth Underwood; Philip B Rawlins; Michael M McCauley; Marie-France Langelier; John M Pascal; Kevin J Embrey; David Neuhaus
Journal:  Nucleic Acids Res       Date:  2021-02-26       Impact factor: 16.971

10.  Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease.

Authors:  Evgeniia Prokhorova; Thomas Agnew; Anne R Wondisford; Michael Tellier; Nicole Kaminski; Danique Beijer; James Holder; Josephine Groslambert; Marcin J Suskiewicz; Kang Zhu; Julia M Reber; Sarah C Krassnig; Luca Palazzo; Shona Murphy; Michael L Nielsen; Aswin Mangerich; Dragana Ahel; Jonathan Baets; Roderick J O'Sullivan; Ivan Ahel
Journal:  Mol Cell       Date:  2021-05-20       Impact factor: 17.970
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