Literature DB >> 21860599

Analysis of Diacylglycerol Molecular Species in Cellular Lipid Extracts by Normal-Phase LC-Electrospray Mass Spectrometry.

Thomas J Leiker1, Robert M Barkley, Robert C Murphy.   

Abstract

The quantitative determination of 48 molecular species of regioisomeric diacylglycerols has been made in a single analysis of an extract of bone marrow derived macrophages. The analytical procedure involves solvent extraction of neutral lipids, including diacylglycerols, derivatization of free hydroxyl moieties as 2,4-difluorophenyl urethane, and analysis by normal phase liquid chromatography-tandem mass spectrometry. The derivatization step not only prevents fatty acyl group migration, thus allowing determination of both 1,2- and 1,3-diacylglycerols, but also yields species that are sensitively and uniquely determined by constant neutral loss mass spectrometry. The method also detected monoacylglycerols, which were characterized by unique retention time and collisional spectra, and were present in mouse bone marrow derived macrophage extracts.

Entities:  

Year:  2011        PMID： 21860599      PMCID： PMC3158596          DOI： 10.1016/j.ijms.2010.09.008

Source DB:  PubMed          Journal:  Int J Mass Spectrom        ISSN： 1387-3806            Impact factor:   1.986


  16 in total

1.  Regio- and stereospecific analysis of glycerolipids.

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2.  Cooperative NCoR/SMRT interactions establish a corepressor-based strategy for integration of inflammatory and anti-inflammatory signaling pathways.

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3.  Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap.

Authors:  Andrew M McAnoy; Christine C Wu; Robert C Murphy
Journal:  J Am Soc Mass Spectrom       Date:  2005-09       Impact factor: 3.109

4.  Quantification of diacylglycerol species from cellular extracts by electrospray ionization mass spectrometry using a linear regression algorithm.

Authors:  Hannah L Callender; Jeffrey S Forrester; Pavlina Ivanova; Anita Preininger; Stephen Milne; H Alex Brown
Journal:  Anal Chem       Date:  2007-01-01       Impact factor: 6.986

Review 5.  CDP-choline:1,2-diacylglycerol cholinephosphotransferase.

Authors:  C R McMaster; R M Bell
Journal:  Biochim Biophys Acta       Date:  1997-09-04

6.  Examining the collision-induced decomposition spectra of ammoniated triglycerides as a function of fatty acid chain length and degree of unsaturation. II. The PXP/YPY series.

Authors:  Xingwen Li; Elizabeth J Collins; Jason J Evans
Journal:  Rapid Commun Mass Spectrom       Date:  2006       Impact factor: 2.419

7.  Analysis of diastereomeric DAG naphthylethylurethanes by normal-phase HPLC with on-line electrospray MS.

Authors:  J J Agren; A Kuksis
Journal:  Lipids       Date:  2002-06       Impact factor: 1.880

8.  Novel chromatographic resolution of chiral diacylglycerols and analysis of the stereoselective hydrolysis of triacylglycerols by lipases.

Authors:  J A Rodriguez; L D Mendoza; F Pezzotti; N Vanthuyne; J Leclaire; R Verger; G Buono; F Carriere; F Fotiadu
Journal:  Anal Biochem       Date:  2007-12-04       Impact factor: 3.365

9.  Circulating cell-free DNA levels in plasma increase with severity in experimental acute pulmonary thromboembolism.

Authors:  Juliana A Uzuelli; Carlos A C Dias-Junior; Tatiane C Izidoro-Toledo; Raquel F Gerlach; Jose E Tanus-Santos
Journal:  Clin Chim Acta       Date:  2009-09-13       Impact factor: 3.786

10.  Separation of cellular nonpolar neutral lipids by normal-phase chromatography and analysis by electrospray ionization mass spectrometry.

Authors:  Patrick M Hutchins; Robert M Barkley; Robert C Murphy
Journal:  J Lipid Res       Date:  2008-01-25       Impact factor: 5.922
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  26 in total

Review 1.  Glycerolipid and cholesterol ester analyses in biological samples by mass spectrometry.

Authors:  Robert C Murphy; Thomas J Leiker; Robert M Barkley
Journal:  Biochim Biophys Acta       Date:  2011-06-26

Review 2.  Mass spectrometry-based shotgun lipidomics - a critical review from the technical point of view.

Authors:  Fong-Fu Hsu
Journal:  Anal Bioanal Chem       Date:  2018-08-09       Impact factor: 4.142

3.  Lipidomic profiling of sinus mucosa from patients with chronic rhinosinusitis.

Authors:  Farbod Fazlollahi; Kessiri Kongmanas; Nongnuj Tanphaichitr; Jon Mallen-St Clair; Quinton Gopen; Kym F Faull; Jeffrey D Suh
Journal:  Clin Transl Sci       Date:  2015-01-15       Impact factor: 4.689

4.  Shotgun Analysis of Diacylglycerols Enabled by Thiol-ene Click Chemistry.

Authors:  Sarju Adhikari; Wenpeng Zhang; Xiaobo Xie; Qinhua Chen; Yu Xia
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5.  Shotgun Lipidomics Approach to Stabilize the Regiospecificity of Monoglycerides Using a Facile Low-Temperature Derivatization Enabling Their Definitive Identification and Quantitation.

Authors:  Kui Yang; Beverly G Dilthey; Richard W Gross
Journal:  Anal Chem       Date:  2016-09-26       Impact factor: 6.986

6.  Inhibition of macrophage fatty acid β-oxidation exacerbates palmitate-induced inflammatory and endoplasmic reticulum stress responses.

Authors:  Dmitry Namgaladze; Sebastian Lips; Thomas J Leiker; Robert C Murphy; Kim Ekroos; Nerea Ferreiros; Gerd Geisslinger; Bernhard Brüne
Journal:  Diabetologia       Date:  2014-02-01       Impact factor: 10.122

7.  Biomarkers of NAFLD progression: a lipidomics approach to an epidemic.

Authors:  D Lee Gorden; David S Myers; Pavlina T Ivanova; Eoin Fahy; Mano R Maurya; Shakti Gupta; Jun Min; Nathanael J Spann; Jeffrey G McDonald; Samuel L Kelly; Jingjing Duan; M Cameron Sullards; Thomas J Leiker; Robert M Barkley; Oswald Quehenberger; Aaron M Armando; Stephen B Milne; Thomas P Mathews; Michelle D Armstrong; Chijun Li; Willie V Melvin; Ronald H Clements; M Kay Washington; Alisha M Mendonsa; Joseph L Witztum; Ziqiang Guan; Christopher K Glass; Robert C Murphy; Edward A Dennis; Alfred H Merrill; David W Russell; Shankar Subramaniam; H Alex Brown
Journal:  J Lipid Res       Date:  2015-01-17       Impact factor: 5.922

8.  Characterization and quantification of diacylglycerol species in biological extracts after one-step derivatization: a shotgun lipidomics approach.

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Journal:  Anal Chem       Date:  2014-01-28       Impact factor: 6.986

Review 9.  Sum of the parts: mass spectrometry-based metabolomics.

Authors:  Stephen B Milne; Thomas P Mathews; David S Myers; Pavlina T Ivanova; H Alex Brown
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10.  Measurements of diacylglycerols in skeletal muscle by atmospheric pressure chemical ionization mass spectrometry.

Authors:  Su-Yeon Lee; Jung Ran Kim; Mi-Young Ha; Soon-Mi Shim; Tae-Sik Park
Journal:  Lipids       Date:  2013-02-08       Impact factor: 1.880
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