A Onanuga1, T C Temedie. 1. Department of Pharmaceutical Microbiology & Biotechnology, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa state, Nigeria. adebolaonanuga@yahoo.co.uk
Abstract
BACKGROUND: Nasal Staphylococcus aureus is a major source of community and hospital associated staphylococcal infections. This study determined the prevalence of nasal S. aureus isolates and investigated their antimicrobial resistance profile in healthy volunteers. METHODS: Nasal specimens of healthy volunteers in Amassoma were cultured and screened for S. aureus using standard microbiological protocols and their antibiotic profile susceptibility was investigated using disc diffusion and agar dilution techniques. RESULTS: A total of 40 (33.3%) S. aureus isolates were obtained from 120 nares specimens screened. Twenty three (57.5%) and 17 (42.5%) of the isolates were from university students and villagers respectively. The isolates showed an overall 75% resistance to ampicillin, 52.5% to doxycycline, 47.5% to chloramphenicol, 35% to erythromycin and 32.5% to cotrimoxazole; with 27.5% methicillin resistant. No isolate was resistant to gentamicin while few isolates were resistant to cefuroxime (2.5%), augmentin (5.0%), ciprofloxacin (10.0%), ofloxacin (10.0%) and vancomycin (7.5%). Twenty one (52.5%) of all the isolates were multi-drug resistant, ten (47.6%) of which were methicillin resistant Staphylococcus aureus (MRSA) and only 3 (7.5%) were fully susceptible to all the tested antimicrobial drugs. CONCLUSIONS: The observation calls for strategies to prevent their spread to more vulnerable populations where the consequences of their infections can be severe.
BACKGROUND: Nasal Staphylococcus aureus is a major source of community and hospital associated staphylococcal infections. This study determined the prevalence of nasal S. aureus isolates and investigated their antimicrobial resistance profile in healthy volunteers. METHODS: Nasal specimens of healthy volunteers in Amassoma were cultured and screened for S. aureus using standard microbiological protocols and their antibiotic profile susceptibility was investigated using disc diffusion and agar dilution techniques. RESULTS: A total of 40 (33.3%) S. aureus isolates were obtained from 120 nares specimens screened. Twenty three (57.5%) and 17 (42.5%) of the isolates were from university students and villagers respectively. The isolates showed an overall 75% resistance to ampicillin, 52.5% to doxycycline, 47.5% to chloramphenicol, 35% to erythromycin and 32.5% to cotrimoxazole; with 27.5% methicillin resistant. No isolate was resistant to gentamicin while few isolates were resistant to cefuroxime (2.5%), augmentin (5.0%), ciprofloxacin (10.0%), ofloxacin (10.0%) and vancomycin (7.5%). Twenty one (52.5%) of all the isolates were multi-drug resistant, ten (47.6%) of which were methicillin resistant Staphylococcus aureus (MRSA) and only 3 (7.5%) were fully susceptible to all the tested antimicrobial drugs. CONCLUSIONS: The observation calls for strategies to prevent their spread to more vulnerable populations where the consequences of their infections can be severe.
Authors: Matthew J Kuehnert; Deanna Kruszon-Moran; Holly A Hill; Geraldine McQuillan; Sigrid K McAllister; Gregory Fosheim; Linda K McDougal; Jasmine Chaitram; Bette Jensen; Scott K Fridkin; George Killgore; Fred C Tenover Journal: J Infect Dis Date: 2005-12-15 Impact factor: 5.226
Authors: T L Smith; M L Pearson; K R Wilcox; C Cruz; M V Lancaster; B Robinson-Dunn; F C Tenover; M J Zervos; J D Band; E White; W R Jarvis Journal: N Engl J Med Date: 1999-02-18 Impact factor: 91.245
Authors: M Ellis; A Serreli; P Colque-Navarro; U Hedstrom; A Chacko; E Siemkowicz; R Möllby Journal: J Med Microbiol Date: 2003-02 Impact factor: 2.472
Authors: Solayide A Adesida; Olusegun A Abioye; Babajide S Bamiro; Bartholomew I C Brai; Stella I Smith; Kehinde O Amisu; Deborah U Ehichioya; Folasade T Ogunsola; Akitoye O Coker Journal: Braz J Infect Dis Date: 2007-02 Impact factor: 1.949
Authors: Fabiana Venegas Pires; Maria de Lourdes Ribeiro de Souza da Cunha; Lígia Maria Abraão; Patrícia Y F Martins; Carlos Henrique Camargo; Carlos Magno Castelo Branco Fortaleza Journal: PLoS One Date: 2014-03-24 Impact factor: 3.240