BACKGROUND: The incidence of coagulase-negative staphylococci reported as causative agents of nosocomial infections has risen in the last decade. The aim of this study was to characterize biofilm formation, antibiotic resistance, SCCmec type, and genetic relatedness in clinical isolates of Staphylococcus cohnii, Staphylococcus hominis, and Staphylococcus sciuri recovered from humans. METHODS: Clinically relevant isolates of S. cohnii (n = 15), S. hominis (n = 9), and S. sciuri (n = 6), were collected from patients. Biofilm formation was evaluated using crystal violet staining, drug susceptibility was assessed using the broth microdilution method, and methicillin resistance was measured using the cefoxitin disk test. SCCmec was typed using 2 different methodologies, and genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE). RESULTS: Sixty percent (9/15) of S. cohnii, 33% (3/9) of S. hominis, and 50% (3/6) of S. sciuri isolates were categorized as weak producers of biofilm. None of the isolates were resistant to vancomycin or linezolid. All 3 species showed a high resistance (> 66%) to ampicillin, levofloxacin, erythromycin, and ceftriaxone, and the majority of the isolates were methicillin-resistant. PFGE revealed that the S. cohnii isolates comprised 1 dominant clone. CONCLUSIONS: The S. cohnii, S. hominis, and S. sciuri isolates analyzed in this study showed a high methicillin resistance and resistance to other antimicrobials. The results of this study strongly suggest that coagulase-negative staphylococci harbour new SCCmec elements. We report the first case of a clone of S. cohnii associated with human disease.
BACKGROUND: The incidence of coagulase-negative staphylococci reported as causative agents of nosocomial infections has risen in the last decade. The aim of this study was to characterize biofilm formation, antibiotic resistance, SCCmec type, and genetic relatedness in clinical isolates of Staphylococcus cohnii, Staphylococcus hominis, and Staphylococcus sciuri recovered from humans. METHODS: Clinically relevant isolates of S. cohnii (n = 15), S. hominis (n = 9), and S. sciuri (n = 6), were collected from patients. Biofilm formation was evaluated using crystal violet staining, drug susceptibility was assessed using the broth microdilution method, and methicillin resistance was measured using the cefoxitin disk test. SCCmec was typed using 2 different methodologies, and genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE). RESULTS: Sixty percent (9/15) of S. cohnii, 33% (3/9) of S. hominis, and 50% (3/6) of S. sciuri isolates were categorized as weak producers of biofilm. None of the isolates were resistant to vancomycin or linezolid. All 3 species showed a high resistance (> 66%) to ampicillin, levofloxacin, erythromycin, and ceftriaxone, and the majority of the isolates were methicillin-resistant. PFGE revealed that the S. cohnii isolates comprised 1 dominant clone. CONCLUSIONS: The S. cohnii, S. hominis, and S. sciuri isolates analyzed in this study showed a high methicillin resistance and resistance to other antimicrobials. The results of this study strongly suggest that coagulase-negative staphylococci harbour new SCCmec elements. We report the first case of a clone of S. cohnii associated with human disease.
Authors: Iulia Lupan; M B Ianc; B S Kelemen; R Carpa; O Rosca-Casian; M T Chiriac; O Popescu Journal: Folia Microbiol (Praha) Date: 2013-07-12 Impact factor: 2.099
Authors: Liangfen Zhang; Jonathan C Thomas; Maria Miragaia; Ons Bouchami; Fernando Chaves; Pedro A d'Azevedo; David M Aanensen; Herminia de Lencastre; Barry M Gray; D Ashley Robinson Journal: PLoS One Date: 2013-06-11 Impact factor: 3.240
Authors: Carlos J Sanchez; Katrin Mende; Miriam L Beckius; Kevin S Akers; Desiree R Romano; Joseph C Wenke; Clinton K Murray Journal: BMC Infect Dis Date: 2013-01-29 Impact factor: 3.090
Authors: Vanessa M Schmidt; Nicola J Williams; Gina Pinchbeck; Caroline E Corless; Stephen Shaw; Neil McEwan; Susan Dawson; Tim Nuttall Journal: BMC Vet Res Date: 2014-01-14 Impact factor: 2.741
Authors: Scott Freeman; Nnadozie O Okoroafor; Christopher M Gast; Mikhail Koval; David Nowowiejski; Eileen O'Connor; Robert D Harrington; John W Parks; Ferric C Fang Journal: J Microbiol Biol Educ Date: 2016-03-01