OBJECTIVE: Interleukin 29 (IL-29) is a class II cytokine and displays numerous immune functions other than its anti-viral and antiproliferation activities. This study is focused on the effect of IL-29 on human keratinocytes (KCs). METHODS: Primary KCs were stimulated by various concentrations of IL-29 for different time periods, and antiviral proteins and TLR3 gene expression were then analyzed by real-time PCR. The signal pathways activated by IL-29 in KCs were detected by western blot. The antiviral activity of IL-29 was determined by methylthiazolyldiphenyl-tetrazolium bromide, and small interfering RNA knockdown was used to analyze the role of toll receptor 3 (TLR3) in the antiviral activity of IL-29. RESULTS: IL-29 was able to induce expression of antiviral proteins and TLR3 gene expression in KCs. IL-29 pretreatment strongly enhanced herpes simplex virus type 1 (HSV-1)-induced expression of the interferon β (IFN-β) gene and protected the KCs from HSV-1 challenge. The IL-29 antiviral activity was partially dependent on TLR3 expression induced by this cytokine, and mechanistic studies demonstrated that the regulation of TLR3 expression by IL-29 might be partially dependent on Janus kinase /signal transducer and activator of transcription (JAK-STATs) activation. CONCLUSION: IL-29-induced TLR3 expression is involved in antiviral activity of IL-29 in KCs, which suggests a feasible method to cure certain viral infections of the skin.
OBJECTIVE:Interleukin 29 (IL-29) is a class II cytokine and displays numerous immune functions other than its anti-viral and antiproliferation activities. This study is focused on the effect of IL-29 on human keratinocytes (KCs). METHODS: Primary KCs were stimulated by various concentrations of IL-29 for different time periods, and antiviral proteins and TLR3 gene expression were then analyzed by real-time PCR. The signal pathways activated by IL-29 in KCs were detected by western blot. The antiviral activity of IL-29 was determined by methylthiazolyldiphenyl-tetrazolium bromide, and small interfering RNA knockdown was used to analyze the role of toll receptor 3 (TLR3) in the antiviral activity of IL-29. RESULTS:IL-29 was able to induce expression of antiviral proteins and TLR3 gene expression in KCs. IL-29 pretreatment strongly enhanced herpes simplex virus type 1 (HSV-1)-induced expression of the interferon β (IFN-β) gene and protected the KCs from HSV-1 challenge. The IL-29 antiviral activity was partially dependent on TLR3 expression induced by this cytokine, and mechanistic studies demonstrated that the regulation of TLR3 expression by IL-29 might be partially dependent on Janus kinase /signal transducer and activator of transcription (JAK-STATs) activation. CONCLUSION:IL-29-induced TLR3 expression is involved in antiviral activity of IL-29 in KCs, which suggests a feasible method to cure certain viral infections of the skin.
Authors: Paul Sheppard; Wayne Kindsvogel; Wenfeng Xu; Katherine Henderson; Stacy Schlutsmeyer; Theodore E Whitmore; Rolf Kuestner; Ursula Garrigues; Carl Birks; Jenny Roraback; Craig Ostrander; Dennis Dong; Jinu Shin; Scott Presnell; Brian Fox; Betty Haldeman; Emily Cooper; David Taft; Teresa Gilbert; Francis J Grant; Monica Tackett; William Krivan; Gary McKnight; Chris Clegg; Don Foster; Kevin M Klucher Journal: Nat Immunol Date: 2002-12-02 Impact factor: 25.606
Authors: Stephan Brand; Florian Beigel; Torsten Olszak; Kathrin Zitzmann; Sören T Eichhorst; Jan-Michel Otte; Joachim Diebold; Helmut Diepolder; Barbara Adler; Christoph J Auernhammer; Burkhard Göke; Julia Dambacher Journal: Am J Physiol Gastrointest Liver Physiol Date: 2005-07-28 Impact factor: 4.052
Authors: Cheng-Lung Ku; Horst von Bernuth; Capucine Picard; Shen-Ying Zhang; Huey-Hsuan Chang; Kun Yang; Maya Chrabieh; Andrew C Issekutz; Coleen K Cunningham; John Gallin; Steven M Holland; Chaim Roifman; Stephan Ehl; Joanne Smart; Mimi Tang; Franck J Barrat; Ofer Levy; Douglas McDonald; Noorbibi K Day-Good; Richard Miller; Hidetoshi Takada; Toshiro Hara; Sami Al-Hajjar; Abdulaziz Al-Ghonaium; David Speert; Damien Sanlaville; Xiaoxia Li; Frédéric Geissmann; Eric Vivier; László Maródi; Ben-Zion Garty; Helen Chapel; Carlos Rodriguez-Gallego; Xavier Bossuyt; Laurent Abel; Anne Puel; Jean-Laurent Casanova Journal: J Exp Med Date: 2007-09-24 Impact factor: 14.307
Authors: Penny A Rudd; Jane Wilson; Joy Gardner; Thibaut Larcher; Candice Babarit; Thuy T Le; Itaru Anraku; Yutaro Kumagai; Yueh-Ming Loo; Michael Gale; Shizuo Akira; Alexander A Khromykh; Andreas Suhrbier Journal: J Virol Date: 2012-07-03 Impact factor: 5.103
Authors: Eduardo I Tognarelli; Tomás F Palomino; Nicolás Corrales; Susan M Bueno; Alexis M Kalergis; Pablo A González Journal: Front Cell Infect Microbiol Date: 2019-04-30 Impact factor: 5.293