OBJECTIVES: To evaluate, in a rat model of glomerulosclerosis, whether ultrasonic shear wave elastography detects kidney cortex stiffness changes and predicts histopathological development of fibrosis. MATERIALS AND METHODS: Three groups were studied transversally: a control group (n = 8), a group after 4 weeks of L-NAME administration (H4, n = 8), and a group after 6 weeks (H6, n = 15). A fourth group was studied longitudinally (n = 8) before, after 4 weeks and after 7 weeks of L-NAME administration. Shear modulus of renal cortex was quantified using supersonic shear imaging technique. Urine was analysed for dosage of protein/creatinine ratio. Kidneys were removed for histological quantification of fibrosis. RESULTS: Diseased rats showed an increased urinary protein/creatinine ratio. Cortical stiffness expressed as median (interquartile range) was 4.0 kPa (3.3-4.5) in control kidneys. It increased in all but one pathological groups: H4: 7.7 kPa (5.5-8.6) (p < 0.01); H6: 4.8 kPa (3.9-5.9) (not significant); in the longitudinal cohort, from 4.5 kPa (3.1-5.9) to 7.7 kPa (5.9-8.3) at week 4 (p < 0.05) and to 6.9 kPa (6.1-7.8) at week 7 (p < 0.05). Stiffness values were correlated with the proteinuria/creatininuria ratio (r = 0.639, p < 0.001). CONCLUSIONS: Increased cortical stiffness is correlated with the degree of renal dysfunction. More experience in other models is necessary to understand its relationship with microstructural changes.
OBJECTIVES: To evaluate, in a rat model of glomerulosclerosis, whether ultrasonic shear wave elastography detects kidney cortex stiffness changes and predicts histopathological development of fibrosis. MATERIALS AND METHODS: Three groups were studied transversally: a control group (n = 8), a group after 4 weeks of L-NAME administration (H4, n = 8), and a group after 6 weeks (H6, n = 15). A fourth group was studied longitudinally (n = 8) before, after 4 weeks and after 7 weeks of L-NAME administration. Shear modulus of renal cortex was quantified using supersonic shear imaging technique. Urine was analysed for dosage of protein/creatinine ratio. Kidneys were removed for histological quantification of fibrosis. RESULTS: Diseased rats showed an increased urinary protein/creatinine ratio. Cortical stiffness expressed as median (interquartile range) was 4.0 kPa (3.3-4.5) in control kidneys. It increased in all but one pathological groups: H4: 7.7 kPa (5.5-8.6) (p < 0.01); H6: 4.8 kPa (3.9-5.9) (not significant); in the longitudinal cohort, from 4.5 kPa (3.1-5.9) to 7.7 kPa (5.9-8.3) at week 4 (p < 0.05) and to 6.9 kPa (6.1-7.8) at week 7 (p < 0.05). Stiffness values were correlated with the proteinuria/creatininuria ratio (r = 0.639, p < 0.001). CONCLUSIONS: Increased cortical stiffness is correlated with the degree of renal dysfunction. More experience in other models is necessary to understand its relationship with microstructural changes.
Authors: Brian J Fahey; Kathryn R Nightingale; Stephen A McAleavey; Mark L Palmeri; Patrick D Wolf; Gregg E Trahey Journal: IEEE Trans Ultrason Ferroelectr Freq Control Date: 2005-04 Impact factor: 2.725
Authors: Robert Arndt; Sven Schmidt; Christoph Loddenkemper; Maria Grünbaum; Walter Zidek; Markus van der Giet; Timm H Westhoff Journal: Transpl Int Date: 2010-02-15 Impact factor: 3.782
Authors: Mireen Friedrich-Rust; Katrin Wunder; Susanne Kriener; Fariba Sotoudeh; Swantje Richter; Joerg Bojunga; Eva Herrmann; Thierry Poynard; Christoph F Dietrich; Johannes Vermehren; Stefan Zeuzem; Christoph Sarrazin Journal: Radiology Date: 2009-08 Impact factor: 11.105
Authors: Nihar S Shah; Scott A Kruse; Donna J Lager; Gerard Farell-Baril; John C Lieske; Bernard F King; Richard L Ehman Journal: Magn Reson Med Date: 2004-07 Impact factor: 4.668
Authors: Patrick A Helm; Peter Caravan; Brent A French; Vincent Jacques; Luhua Shen; Yaqin Xu; Ronald J Beyers; R Jack Roy; Christopher M Kramer; Frederick H Epstein Journal: Radiology Date: 2008-04-10 Impact factor: 11.105
Authors: Thomas Payen; Carmine F Palermo; Stephen A Sastra; Hong Chen; Yang Han; Kenneth P Olive; Elisa E Konofagou Journal: Phys Med Biol Date: 2016-07-12 Impact factor: 3.609