BKM-react, an integrated biochemical reaction database.

BACKGROUND: The systematic, complete and correct reconstruction of genome-scale metabolic networks or metabolic pathways is one of the most challenging tasks in systems biology research. An essential requirement is the access to the complete biochemical knowledge - especially on the biochemical reactions. This knowledge is extracted from the scientific literature and collected in biological databases. Since the available databases differ in the number of biochemical reactions and the annotation of the reactions, an integrated knowledge resource would be of great value.
RESULTS: We developed a comprehensive non-redundant reaction database containing known enzyme-catalyzed and spontaneous reactions. Currently, it comprises 18,172 unique biochemical reactions. As source databases the biochemical databases BRENDA, KEGG, and MetaCyc were used. Reactions of these databases were matched and integrated by aligning substrates and products. For the latter a two-step comparison using their structures (via InChIs) and names was performed. Each biochemical reaction given as a reaction equation occurring in at least one of the databases was included.
CONCLUSIONS: An integrated non-redundant reaction database has been developed and is made available to users. The database can significantly facilitate and accelerate the construction of accurate biochemical models.

Background

For the construction of cellular models, the development of organism-specific reaction networks is essential. A number of sources for biochemical reactions exist, as the databases BRENDA [1], KEGG [2], and MetaCyc [3]. In general, the integration of biological databases is not trivial [4]. Due to the fact that the completeness of reaction data differs between the databases, it becomes important to combine the available reaction information of the used source databases in form of an integrated reaction database.

So a combination will lead to more complete and reliable metabolic networks. Therefore it is necessary to find identical reactions between the recognized databases. As different compound names and compound IDs, as well as reaction IDs, are in use within the described biochemical reactions a comparison is far from straightforward. A major obstacle results from the use of generic compound names, e.g. 'an aldehyde' or 'an alcohol'. Furthermore some reactions even occur in the same database twice with different reaction IDs.

Integrated databases exist for diverse biological topics. The TRANSPATHdatabase for example is an integrated database which deals with signal transduction information [5]. As an example for an integrated metabolic database system the database BioSilico can be mentioned here [6]. For creation of this database, information of the metabolic databases KEGG, ENZYME [7], EcoCyc [8], and MetaCyc was combined, the latter two building parts of BioCyc [9]. The database BioSilico includes information on enzymes, biochemical compounds, and reactions. Radrich et al. [10] provide a semi-automated tool for the process of genome-scale network reconstruction demonstrated on the basis of data for Arabidopsis thaliana. Their integrated data set is built on the two sources KEGG and AraCyc [11]. Furthermore a reaction database on human biological pathways and processes named Reactome [12] exists as well as an annotated reaction database called Rhea [13], basically a modified version of the reactions defined in the IUBMB enzyme list [14]. A collection of biochemical reactions and pathways in printed form contains the book Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology [15].

Methods

In this work information from the biological databases BRENDA [1], KEGG [2], and MetaCyc [3] was used (May 2011). Reaction comparisons were done by an in silico approach in which two steps, first a comparison of reactant structures using InChIs (linearized chemical structure descriptors [16]) and, second, a compound name comparison (incl. synonyms), were combined. An InChI structure coding was generated based on an original Molfile (contains molecular structure information [17]) by using a special converting tool (InChI version 1 (software version 1.03) for Standard and Non-Standard InChI/InChIKey [18]). By using only relevant layers of an self-generated InChI, a higher matching rate was achieved. For this purpose we dropped the InChI layers dependent on the ionisation state so that e.g. acetic acid and the acetate ion were considered to be the same compound. Reactions without EC numbers were included as well as those reactions with incomplete EC numbers. Spontaneous reactions without EC number were labelled SPONTANEOUS. Before the comparison, the compounds water (H2O) and proton (H+) were removed from the reactions. Additionally, a stoichiometry check was executed. This information was added as attribute to the reactions in the database as a quality measure. Stoichiometrically imbalanced reactions were marked as incomplete in the column Stoichiometry, except in cases where only a proton or water is missing. In two supplemental columns the incomplete cases are differentiated into Missing Substrate and Missing Product.

For the compound name based comparison step all found synonyms were used as well as generated 'DAYLIGHT names' (Chemical Information Systems, Inc. [19]). We applied a special conversion that removed most of the common sources of differences in equivalent compound names like hyphens, parentheses, etc. Most of the special characters, except '+' and apostrophe ('), were deleted. For identifying common reactions, all available synonyms and 'DAYLIGHT names' (see above) of the compounds are included in form of a link table containing assigned compound IDs. Where possible, KEGG glycan IDs (G number) were exchanged by their corresponding compound IDs (C number). Reactions with NAD(P)/H (BRENDA) and NADP/H_OR_NO_P (MetaCyc) were split into two reactions, one with NADH, the other with NADPH. The reaction ID of the form without phosphate was labelled as the original but with _WOP (= WithOut Phosphate) at the end.

Data download, storage, and comparison was realized by C++ as well as Python scripts and embedded MySQL statements. By executing a cron-job in regular time points, the information about metabolites, enzymes, reactions, Molfiles, and InChIs was downloaded from the source databases and so kept up to date automatically.

The access to the integrated database is possible via the link to BKM-react [20], Figure 1A, or via the BRENDA website, making use of the BRENDA query engine. Figure 1B illustrates the access to the integrated non-redundant reaction database [21] → Reaction & Specificity → Biochemicals Reactions Aligned (see arrow). Parameters for doing queries are presented in Figure 2A for the reaction table. Figure 2B shows an example for a query result. The downloadable content of the database consists of three tables, containing the compared reactions, the according compounds as well as a link table connecting both with each other.

Figure 1

Link to the integrated non-redundant reaction database. Website of BKM-react (A) and the BRENDA main menu with the link (B, see arrow).

Figure 2

. Complete menu for the MySQL query of the reaction table (A) and example query (B).

Results and discussion

The combined database contains a unique list of reactions that occur in any of the compared databases BRENDA [1], KEGG [2], and MetaCyc [3] and the associations between equivalent reactions. Additionally these reactions are assigned to KEGG and MetaCyc pathways. Table 1 lists the data used for the comparison. The largest number of reactions originates from the BRENDA database, followed by MetaCyc, and KEGG.

Table 1

Overview reaction sources and data

	DifferentEC numbers	IncompleteEC numbers	Reaction IDs	Reaction IDswithout EC number	Compound IDs	Synonyms	Molfiles	InChIs
KEGG	3,761	122	8,452	1,288	6,522	11,597	6,327	5,416
MetaCyc	4,159	138	9,343	2,236	6,095	19,707	6,035	5,782
BRENDA	4,425	207	10,109	55	9,750	20,922	9,750	5,242

The number of reactions in BRENDA is in fact close to 180,000. In this case only complete reactions with natural substrates were included.

A significantly improved matching of reactions was achieved by removing the compounds H+ and water (H2O) from the reactions before comparing them because the reactions in the databases are not always stoichiometrically balanced. The order of executing first the InChI comparison followed by the name comparison was chosen because identical synonyms may occur for different compounds. To rely on synonyms could therefore result in incorrect links. By using the reverse order more false positive matchings would appear.

One of the difficulties in the comparison consists in the - sometimes implied - stereochemistry not given in the compound name. Whereas cases like "alanine" being used for "L-alanine" are obviously to be expected, sometimes things become more complicated. For example, in BRENDA and MetaCyc beta-stereochemistry is implied for C5 of D-fructose-1,6-bisphosphate, being the major stereoisomer (see Figure 3A and 3B), the KEGG database includes in fact two different reactions, one with beta-stereochemistry at C5, the other with undefined stereochemistry (see Figure 4A and 4B) where pathway information is only assigned to the reaction with the full stereochemistry. In general metabolites with complete stereochemistry are favored in BKM-react.

Figure 3

Screenshots of the databases . These reactions, BRENDA reaction BR47724 and the MetaCyc reaction 6PFRUCTPHOS-RXN are matching the KEGG reaction R04779 (Fig. 4 B) because of the complete InChI string for beta-D-Fructose 1,6-bisphosphate even if MetaCyc names it D-fructose-6-phosphate.

Figure 4

Screenshots of the database . KEGG R00756 and R04779. The second reaction is the preferred one. C04779 possesses the complete InChI string and is therefore matched with the more complete described metabolites of the other databases.

If no structural information is available, reactions are allowed to match by name comparison.

This example shows a general problem in biochemical compound name comparison. The large majority of biochemists refer to S-alanine just by the name alanine although the name in principle is ambiguous or should be used for the racemate. In most cases we assume that for the standard amino acids the name without stereo-descriptor implicitly means S- (or L-, respectively). This holds true also for some other compound names where the stereo-descriptor is implicitly given. A related problem occurs at positions where the stereochemistry is ambiguous like in the case of C1 of D-glucose. In some cases the stereochemistry for this position is undefined in the Molfiles [17], in others the more stable form (e.g. beta in the case of glucose) is used and defined.

Although all three databases offer their own InChIs, they are not directly comparable because KEGG uses the non-standard form of an InChI, whereby MetaCyc and BRENDA use the standard InChI format. So for a standardized comparison it is necessary to use self-generated InChIs based on Molfiles. For this purpose the official IUPAC converting tool was utilized [18]. A higher matching rate was achieved by using only essential layers (see Methods section) of an InChI string. A drawback is that not for each compound an InChI is available, e.g. for macromolecular reactants or for generic compounds.

A pairwise comparison of reactions revealed a high identity between KEGG &MetaCyc. About 50% reactions were equal, out of which most were also found in BRENDA (Figure 5). Between MetaCyc &BRENDA 3,174 reactions were identified to be equal. Comparing KEGG &BRENDA, even more reactions (3,617) could be assigned to each other.

Figure 5

Distribution of unique reactions between the used databases.

Table 2 shows the assignment of diverse reactions between the databases which are equal. There are examples of reactions that have a 1:n relation because of redundant reactions occurring within the same database. In KEGG for example, metabolites are differentiated into glycans and compounds, respectively. This means that identical compounds may get two different IDs, starting with G and C. This results in reactions with different reaction IDs (no. 3 in Tab. 2). Sometimes there are synonyms or keto-enol tautomers which describe one reaction in various forms (no. 1 in Tab. 2) or other alternative writing styles (no. 2 in Tab. 2). Further KEGG uses one reaction-ID for the same reaction being catalysed by enzymes with different EC numbers, whereas MetaCyc often uses different reaction IDs in such cases (no. 1 in Tab. 2).

Table 2

Some instructive cases of different forms for identical reactions

No.	KEGG	MetaCyc	BRENDA	Definition
1	R04915			Quinoline-3,4-diol + Oxygen <=> Formylanthranilate + CO
	R05719			3-Hydroxy-1H-quinolin-4-one + Oxygen <=> Formylanthranilate + CO
		1.13.11.47-RXN		3-hydroxy-1H-quinolin-4-one + oxygen = carbon monoxide + N-formylanthranilate
			BR22597	3-hydroxy-1H-quinolin-4-one + O2 = N- formylanthranilate + CO
2	R00004			Diphosphate + H2O <=> 2 Orthophosphate
		INORGPYROPHOSPHAT-RXN		diphosphate + H2O = 2 phosphate + H+
			BR22749	diphosphate + H2O = 2 phosphate
3	R00010			alpha, alpha-Trehalose + H2O <=> 2 D-Glucose (C01083)
	R06103			Trehalose + H2O <=> 2 D-Glucose (G00293)
		TREHALA-RXN		trehalose + H2O → 2 β-D-glucose
			BR15991	alpha, alpha-trehalose + H2O = 2 D-glucose
			BS370856	alpha, alpha-trehalose + H2O = beta-Dglucose

In Figure 5 the distribution of equal reactions occurring in any of the three databases is illustrated. 2,890 of all reactions are contained in all three databases, corresponding to 34% of all KEGG reactions, 31% of all MetaCyc reactions, and 29% of the included BRENDA reactions, respectively. In the present version of the data set, 3,743 KEGG reactions, 5,038 MetaCyc reactions, and 4,606 BRENDA reactions occur only in the respective database (Figure 5). Altogether the non-redundant reaction database up to now contains 18,172 unique reactions and 20,358 EC/reaction combinations as some reactions are catalyzed by a number of different enzymes.

In Figure 6 the fraction of all unique reactions belonging to the six main EC classes is shown. The largest fractions belong to EC classes 1 and 2, followed by class 3. Statistical data about the EC numbers occurring in the non-redundant reaction database are given in Table 3. Additionally to all EC numbers, complete and incomplete, the latter ones are listed separately. Furthermore it is distinguished between EC numbers representing at least one single reaction or more than one. A detailed look on the EC numbers with the highest number of reactions is given in Table 4 together with the number of reactions.

Figure 6

Fraction [%] of unique reactions in main EC classes (1-6).

Table 3

Statistics about EC numbers occurring in the integrated non-redundant reaction database

EC numbers	Different EC numbers	Incomplete EC numbers
in total	4,288	365
with > 1 reaction	2,681	185
with > 5 reactions	561	73
with > 10 reactions	184	49

Table 4

Complete EC numbers with the highest number of reactions

EC number	Enzyme	Number of reactions
1.14.14.1	unspecific monooxygenase	116
2.4.1.17	glucuronosyltransferase	80
3.2.1.21	beta-glucosidase	74
1.1.1.100	3-oxoacyl-[acyl-carrier-protein] reductase	55
3.5.1.4	amidase	46
3.6.3.44	xenobiotic-transporting ATPase	46
3.1.3.16	phosphoprotein phosphatase	44
1.3.1.10	enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific)	43
3.2.1.1	alpha-amylase	43
1.1.1.50	3alpha-hydroxysteroid dehydrogenase (B-specific)	42
2.3.1.41	beta-ketoacyl-acyl-carrier-protein synthase I	41
3.6.1.9	nucleotide diphosphatase	39
1.1.1.1	alcohol dehydrogenase	37
2.3.1.86	fatty-acyl-CoA synthase	37
1.14.13.72	methylsterol monooxygenase	36
1.2.1.3	aldehyde dehydrogenase (NAD+)	34
1.2.1.5	aldehyde dehydrogenase [NAD(P)+]	33
3.2.1.24	alpha-mannosidase	33
3.2.1.51	alpha-L-fucosidase	33
1.14.13.8	flavin-containing monooxygenase	32
1.4.3.3	D-amino-acid oxidase	32

Recommended names of enzymes: source BRENDA database.

The only database with a similar goal is BioSilico [6]. One important difference consists of the fact that the assignment of identical reactions in our database is done by an actual comparison of the compounds structure in combination with synonyms whereas in BioSilico, the matching is only a simple assignment of reactions having the same EC number without redundancy check.

The number of reactions in the database described in this paper is far beyond that in BioSilico. Selecting three EC numbers by chance resulted in e.g. EC number 1.14.14.1 → 4 reactions in BioSilico vs. 116 reactions in our reaction database, EC number 2.1.1.103 → 1 reaction in BioSilico vs. 4 reactions in our database, 3.1.1.47 → 1 reaction in BioSilico vs. 12 reactions in our database. The fact that in these examples not even all available KEGG reactions were found in BioSilico indicates that this database is not updated.

Additionally, our reaction database contains the information whether a reaction is stoichiometric incomplete or not. This test is performed before the removal of H+ and H2O. Non-balanced reactions are labeled in a separate table column. 2,803 out of 18,172 reactions are at present in this category. The labeling allows modelers to select only balanced reactions for the reconstruction of organism-specific models and networks.

The tool of Radrich et al. [10] also includes a stoichiometric evaluation. Their method includes a name comparison where they compare the similarity of compound names. Further they use SMILES strings for a structural comparison. The tool was executed only for Arabidopsis thaliana, so no general comparison could be done. For this purpose the authors combined data of the databases KEGG and AraCyc [11].

Conclusions

In this work we present an integrated and non-redundant reaction database implementing a combined approach of structure and name based comparison.

The tool, integrated into the BRENDA [1] query engine but not confined to BRENDA data is offering a novel valuable tool that can be used e.g. for the construction of biological models. The resulting models will be much more complete than if only one of the databases is used as the three biological databases BRENDA, KEGG [2], and MetaCyc [3] complement each other. Regular 6-monthly updates of this database will make guarantee actuality.

Availability and requirements

The integrated and non-redundant reaction database is accessible via BKM-react [20] and the website of the BRENDA [1] database: BRENDA website [21] → Reaction & Specificity → Biochemicals Reactions Aligned (Figure 1). The complete dataset is additionally provided as a CSV-formatted download at the same site. Available is a reaction table, a table with all compounds occurring in the reactions, and an assignment table with the linkage between reactions and compounds.

List of abbreviations used

BRENDA: aunschweig zyme tabase; EC: nzyme ommission; InChI: IUPAC ternational emical dentifier; IUBMB: nternational nion of iochemistry and olecular iology; IUPAC: nternational nion of ure and pplied hemistry; KEGG: yoto ncyclopedia of enes and enomes; SMILES: implified olecular nput ine ntry ystem.

Authors' contributions

ML and MS executed the data acquisition and implemented the reaction comparison. ML and MS were involved in the construction of the integrated reaction database and the scientific evaluation. DS had the idea to develop the reaction database and supervised the development. ML, MS, and DS wrote the manuscript. All authors read and approved the final manuscript.