AIMS: To evaluate the inactivation of Bacillus anthracisΔSterne and Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO(2)) and compare two sporulation and decontamination methods with regard to cost, safety and technical constraints. METHODS AND RESULTS: Spores were prepared via agar and broth methods and subsequently inoculated and dried onto clean, autoclave-sterilized glass coupons. Bacillus anthracis spore inactivation efficacy was evaluated using the modified three-step method (AOAC 2008.05) and a single-tube extraction method. Spores (7·0 ± 0·5 logs) were inactivated within 1 min at room temperature using freshly prepared eClO(2). Bacillus anthracisΔSterne spores decreased in size after eClO(2) treatment as measured using a Beckman Coulter Multisizer. CONCLUSIONS: eClO(2) saturation of a hard surface was an effective B. anthracis sporicide. Broth sporulation and the single-tube extraction method required less time and fewer steps, yielded a higher percentage of phase-bright spores and showed higher spore recovery efficiency compared with AOAC 2008.05, making it more amenable to biosafety level 3 (BSL3) testing of virulent spores. SIGNIFICANCE AND IMPACT OF THE STUDY: Two test methods demonstrated the sporicidal efficacy of eClO(2). A new single-tube extraction test protocol for decontaminants was introduced. Journal of Applied Microbiology
AIMS: To evaluate the inactivation of Bacillus anthracisΔSterne and Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO(2)) and compare two sporulation and decontamination methods with regard to cost, safety and technical constraints. METHODS AND RESULTS: Spores were prepared via agar and broth methods and subsequently inoculated and dried onto clean, autoclave-sterilized glass coupons. Bacillus anthracis spore inactivation efficacy was evaluated using the modified three-step method (AOAC 2008.05) and a single-tube extraction method. Spores (7·0 ± 0·5 logs) were inactivated within 1 min at room temperature using freshly prepared eClO(2). Bacillus anthracisΔSterne spores decreased in size after eClO(2) treatment as measured using a Beckman Coulter Multisizer. CONCLUSIONS: eClO(2) saturation of a hard surface was an effective B. anthracis sporicide. Broth sporulation and the single-tube extraction method required less time and fewer steps, yielded a higher percentage of phase-bright spores and showed higher spore recovery efficiency compared with AOAC 2008.05, making it more amenable to biosafety level 3 (BSL3) testing of virulent spores. SIGNIFICANCE AND IMPACT OF THE STUDY: Two test methods demonstrated the sporicidal efficacy of eClO(2). A new single-tube extraction test protocol for decontaminants was introduced. Journal of Applied Microbiology
Authors: Christopher K Cote; Tony Buhr; Casey B Bernhards; Matthew D Bohmke; Alena M Calm; Josephine S Esteban-Trexler; Melissa Hunter; Sarah E Katoski; Neil Kennihan; Christopher P Klimko; Jeremy A Miller; Zachary A Minter; Jerry W Pfarr; Amber M Prugh; Avery V Quirk; Bryan A Rivers; April A Shea; Jennifer L Shoe; Todd M Sickler; Alice A Young; David P Fetterer; Susan L Welkos; Joel A Bozue; Derrell McPherson; Augustus W Fountain; Henry S Gibbons Journal: Appl Environ Microbiol Date: 2018-05-31 Impact factor: 4.792
Authors: Christopher K Cote; Jessica M Weidner; Christopher Klimko; Ashley E Piper; Jeremy A Miller; Melissa Hunter; Jennifer L Shoe; Jennifer C Hoover; Brian R Sauerbry; Tony Buhr; Joel A Bozue; David E Harbourt; Pamela J Glass Journal: Appl Biosaf Date: 2021-03-19
Authors: Anthony Friedline; Malcolm Zachariah; Amy Middaugh; Matt Heiser; Neeraj Khanna; Parag Vaishampayan; Charles V Rice Journal: AMB Express Date: 2015-04-17 Impact factor: 3.298