Literature DB >> 218224

Transfection of human lymphoblastoid cells with herpes simplex viral DNA.

G Miller, P Wertheim, G Wilson, J Robinson, J L Geelen, J van der Noordaa, A J van der Eb.   

Abstract

The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.

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Year:  1979        PMID： 218224      PMCID： PMC383102          DOI： 10.1073/pnas.76.2.949

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Studies on the Epstein-Barr Virus genome and the EBV-determined nuclear antigen in human malignant disease.

Authors:  G Klein
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

2.  Radiobiological inactivation of Epstein-Barr virus.

Authors:  E Henderson; L Heston; E Grogan; G Miller
Journal:  J Virol       Date:  1978-01       Impact factor: 5.103

3.  Human cytomegalovirus DNA. I. Molecular weight and infectivity.

Authors:  J L Geelen; C Walig; P Wertheim; J van der Noordaa
Journal:  J Virol       Date:  1978-06       Impact factor: 5.103

4.  Infectious herpesvirus DNA.

Authors:  F L Graham; G Veldhuisen; N M Wilkie
Journal:  Nat New Biol       Date:  1973-10-31

5.  Fatal encephalitis due to Herpesvirus hominis: use of intact brain cells for isolation of virus.

Authors:  T C Shope; J Klein-Robbenhaar; G Miller
Journal:  J Infect Dis       Date:  1972-05       Impact factor: 5.226

6.  Effect of herpes simplex virus on cultured Burkitt tumor cells and its failure to influence the Epstein-Barr virus carrier state.

Authors:  W Henle; G Henle; H zur Hausen
Journal:  Cancer Res       Date:  1969-02       Impact factor: 12.701

7.  Infectious DNA from herpes simplex virus: infectivity of double-stranded and single-stranded molecules.

Authors:  P Sheldrick; M Laithier; D Lando; M L Ryhiner
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

8.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891

9.  Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas.

Authors:  G Klein; T Lindahl; M Jondal; W Leibold; J Menézes; K Nilsson; C Sundström
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

10.  Differential reactivity of human serums with early antigens induced by Epstein-Barr virus.

Authors:  W Henle; G Henle; B A Zajac; G Pearson; R Waubke; M Scriba
Journal:  Science       Date:  1970-07-10       Impact factor: 47.728
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  9 in total

1.  Cell-type-specific synthesis of murine immunoglobulin mu RNA from an adenovirus vector.

Authors:  J E Ruether; A Maderious; D Lavery; J Logan; S M Fu; S Chen-Kiang
Journal:  Mol Cell Biol       Date:  1986-01       Impact factor: 4.272

2.  Latent infections in spinal ganglia with thymidine kinase-deficient herpes simplex virus.

Authors:  T P Leist; R M Sandri-Goldin; J G Stevens
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

3.  Transfection of a rearranged viral DNA fragment, WZhet, stably converts latent Epstein-Barr viral infection to productive infection in lymphoid cells.

Authors:  E Grogan; H Jenson; J Countryman; L Heston; L Gradoville; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

4.  Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.

Authors:  Y M Shen; R R Hirschhorn; W E Mercer; E Surmacz; Y Tsutsui; K J Soprano; R Baserga
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272

5.  Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA.

Authors:  A Graessmann; H Wolf; G W Bornkamm
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

6.  Efficient introduction of plasmid DNA into human hemopoietic cells by encapsidation in simian virus 40 pseudovirions.

Authors:  A Oppenheim; A Peleg; E Fibach; E A Rachmilewitz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

7.  Localization on the herpes simplex virus type 1 genome of a region encoding proteins involved in adsorption to the cellular receptor.

Authors:  N Langeland; A M Oyan; H S Marsden; A Cross; J C Glorioso; L J Moore; L Haarr
Journal:  J Virol       Date:  1990-03       Impact factor: 5.103

8.  Resistance of herpes simplex virus type 2 to neomycin maps to the N-terminal portion of glycoprotein C.

Authors:  A M Oyan; K E Dolter; N Langeland; W F Goins; J C Glorioso; L Haarr; C S Crumpacker
Journal:  J Virol       Date:  1993-05       Impact factor: 5.103

9.  Pleiotropic expression of Epstein--Barr virus DNA in human epithelial cells.

Authors:  J Stoerker; D Parris; Y Yajima; R Glaser
Journal:  Proc Natl Acad Sci U S A       Date:  1981-09       Impact factor: 11.205
  9 in total

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