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Literature DB >> 21786833

Dual-label STED nanoscopy of living cells using photochromism.

Katrin I Willig¹, Andre C Stiel, Tanja Brakemann, Stefan Jakobs, Stefan W Hell.

Abstract

We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.

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Year: 2011 PMID： 21786833 DOI： 10.1021/nl202290w

Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189

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Cited

13 in total

1. Two-color STED microscopy of living synapses using a single laser-beam pair.

Authors: Jan Tønnesen; Fabien Nadrigny; Katrin I Willig; Roland Wedlich-Söldner; U Valentin Nägerl
Journal: Biophys J Date: 2011-11-15 Impact factor: 4.033

2. Live-cell imaging.

Authors: Richard Cole
Journal: Cell Adh Migr Date: 2014-10-31 Impact factor: 3.405

3. Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.

Authors: Kyu Young Han; Taekjip Ha
Journal: Opt Lett Date: 2015-06-01 Impact factor: 3.776

4. Two-photon excitation STED microscopy in two colors in acute brain slices.

Authors: Philipp Bethge; Ronan Chéreau; Elena Avignone; Giovanni Marsicano; U Valentin Nägerl
Journal: Biophys J Date: 2013-02-19 Impact factor: 4.033

Review 5. Fluorescence nanoscopy. Methods and applications.

Authors: Jose Requejo-Isidro
Journal: J Chem Biol Date: 2013-06-04

6. STAQ: A route toward low power, multicolor nanoscopy.

Authors: Tilman Rosales; Dan L Sackett; Jianhua Xu; Zhen-Dan Shi; Biying Xu; Haitao Li; Gurpreet Kaur; Erin Frohart; Nalini Shenoy; Sarah M Cheal; Haitao Wu; Andrés E Dulcey; Yulin Hu; Changhui Li; Kelly Lane; Gary L Griffiths; Jay R Knutson
Journal: Microsc Res Tech Date: 2015-03-12 Impact factor: 2.769

Dual-label STED nanoscopy of living cells using photochromism.

1. Two-color STED microscopy of living synapses using a single laser-beam pair.

2. Live-cell imaging.

3. Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.

4. Two-photon excitation STED microscopy in two colors in acute brain slices.

Review 5. Fluorescence nanoscopy. Methods and applications.

6. STAQ: A route toward low power, multicolor nanoscopy.

7. Radial sizing of lipid nanotubes using membrane displacement analysis.

Review 8. Interferon gamma receptor: the beginning of the journey.

9. rsEGFP2 enables fast RESOLFT nanoscopy of living cells.

10. 4Pi-RESOLFT nanoscopy.