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Literature DB >> 21765410

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases.

Fuqiang Chen¹, Shondra M Pruett-Miller, Yuping Huang, Monika Gjoka, Katarzyna Duda, Jack Taunton, Trevor N Collingwood, Morten Frodin, Gregory D Davis.

Abstract

Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mutation, (ii) targeted genomic deletion of up to 100 kb and (iii) targeted insertion of small genetic elements concomitant with large genomic deletions.

Entities: Chemical

Mesh：

Substances：

Year: 2011 PMID： 21765410 PMCID： PMC3617923 DOI： 10.1038/nmeth.1653

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

18 in total

1. Enhancing gene targeting with designed zinc finger nucleases.

Authors: Marina Bibikova; Kelly Beumer; Jonathan K Trautman; Dana Carroll
Journal: Science Date: 2003-05-02 Impact factor: 47.728

2. Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast.

Authors: Francesca Storici; Christopher L Durham; Dmitry A Gordenin; Michael A Resnick
Journal: Proc Natl Acad Sci U S A Date: 2003-11-20 Impact factor: 11.205

3. Double-stranded break can be repaired by single-stranded oligonucleotides via the ATM/ATR pathway in mammalian cells.

Authors: Zai Wang; Zhong-Jun Zhou; De-Pei Liu; Jian-Dong Huang
Journal: Oligonucleotides Date: 2008

4. Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: wanted and unwanted target locus modifications.

Authors: Sarah Radecke; Frank Radecke; Toni Cathomen; Klaus Schwarz
Journal: Mol Ther Date: 2010-01-12 Impact factor: 11.454

5. Targeted chromosomal deletions in human cells using zinc finger nucleases.

Authors: Hyung Joo Lee; Eunji Kim; Jin-Soo Kim
Journal: Genome Res Date: 2009-12-01 Impact factor: 9.043

6. Highly efficient endogenous human gene correction using designed zinc-finger nucleases.

Authors: Fyodor D Urnov; Jeffrey C Miller; Ya-Li Lee; Christian M Beausejour; Jeremy M Rock; Sheldon Augustus; Andrew C Jamieson; Matthew H Porteus; Philip D Gregory; Michael C Holmes
Journal: Nature Date: 2005-04-03 Impact factor: 49.962

7. Gene conversion tracts from double-strand break repair in mammalian cells.

Authors: B Elliott; C Richardson; J Winderbaum; J A Nickoloff; M Jasin
Journal: Mol Cell Biol Date: 1998-01 Impact factor: 4.272

8. Structural bioinformatics-based design of selective, irreversible kinase inhibitors.

Authors: Michael S Cohen; Chao Zhang; Kevan M Shokat; Jack Taunton
Journal: Science Date: 2005-05-27 Impact factor: 47.728

9. Cellular responses to targeted genomic sequence modification using single-stranded oligonucleotides and zinc-finger nucleases.

Authors: Petter Angell Olsen; Anita Solhaug; James Alexander Booth; Monika Gelazauskaite; Stefan Krauss
Journal: DNA Repair (Amst) Date: 2008-12-30

10. Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases.

Authors: Pei-Qi Liu; Edmond M Chan; Gregory J Cost; Lin Zhang; Jianbin Wang; Jeffrey C Miller; Dmitry Y Guschin; Andreas Reik; Michael C Holmes; John E Mott; Trevor N Collingwood; Philip D Gregory
Journal: Biotechnol Bioeng Date: 2010-05-01 Impact factor: 4.530

215 in total

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases.

1. Enhancing gene targeting with designed zinc finger nucleases.

2. Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast.

3. Double-stranded break can be repaired by single-stranded oligonucleotides via the ATM/ATR pathway in mammalian cells.

4. Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: wanted and unwanted target locus modifications.

5. Targeted chromosomal deletions in human cells using zinc finger nucleases.

6. Highly efficient endogenous human gene correction using designed zinc-finger nucleases.

7. Gene conversion tracts from double-strand break repair in mammalian cells.

8. Structural bioinformatics-based design of selective, irreversible kinase inhibitors.

9. Cellular responses to targeted genomic sequence modification using single-stranded oligonucleotides and zinc-finger nucleases.

10. Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases.

1. Modeling disease mutations by gene targeting in one-cell mouse embryos.

2. The iCRISPR platform for rapid genome editing in human pluripotent stem cells.

Review 3. Targeting protein kinases with selective and semipromiscuous covalent inhibitors.

4. Genome editing in human stem cells.

5. Gene editing: not just for translation anymore.

6. The author file: Greg Davis.

7. Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.

Review 8. Salient Features of Endonuclease Platforms for Therapeutic Genome Editing.

9. Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing.

Review 10. Induced pluripotency as a potential path towards iNKT cell-mediated cancer immunotherapy.