OBJECTIVE: The purpose of this study was to examine the histologic and immunologic differences between fetal membrane zones after membrane rupture at term delivery. STUDY DESIGN: Fetal membrane explants from postrupture zones (periplacental, middle, rupture) were obtained from women following spontaneous vaginal delivery at term (n = 5). Tissues for histology, protein extracts, and RNA were isolated. RESULTS: The collagen distribution decreased and the leukocyte density increased from the periplacental zone to the rupture zone. T cells were mainly present in the rupture zone and granulocytes in the middle zone. CXCL10, CXCR1, ICAM-1, -2, PSEL, tumor necrosis factor alpha, and matrix metalloproteinase-9 levels were higher in the middle zone than in the rupture zone and periplacental zone (P < .021). Interleukin-1beta and CXCL8 levels were higher in the rupture zone than in the middle zone and periplacental zone (P = .018 and P < .0001). CONCLUSION: During labor specific immunologic microenvironments are created in the zones of the fetal membrane that may be involved in their rupture at the end of gestation.
OBJECTIVE: The purpose of this study was to examine the histologic and immunologic differences between fetal membrane zones after membrane rupture at term delivery. STUDY DESIGN: Fetal membrane explants from postrupture zones (periplacental, middle, rupture) were obtained from women following spontaneous vaginal delivery at term (n = 5). Tissues for histology, protein extracts, and RNA were isolated. RESULTS: The collagen distribution decreased and the leukocyte density increased from the periplacental zone to the rupture zone. T cells were mainly present in the rupture zone and granulocytes in the middle zone. CXCL10, CXCR1, ICAM-1, -2, PSEL, tumor necrosis factor alpha, and matrix metalloproteinase-9 levels were higher in the middle zone than in the rupture zone and periplacental zone (P < .021). Interleukin-1beta and CXCL8 levels were higher in the rupture zone than in the middle zone and periplacental zone (P = .018 and P < .0001). CONCLUSION: During labor specific immunologic microenvironments are created in the zones of the fetal membrane that may be involved in their rupture at the end of gestation.
Authors: Jose Galaz; Roberto Romero; Yi Xu; Derek Miller; Dustyn Levenson; Robert Para; Aneesha Varrey; Richard Hsu; Anna Tong; Sonia S Hassan; Chaur-Dong Hsu; Nardhy Gomez-Lopez Journal: J Perinat Med Date: 2020-09-25 Impact factor: 1.901
Authors: D Levenson; R Romero; V Garcia-Flores; D Miller; Y Xu; A Sahi; S S Hassan; N Gomez-Lopez Journal: Clin Exp Immunol Date: 2020-05-07 Impact factor: 4.330
Authors: Jodi K Regan; Paranthaman S Kannan; Matthew W Kemp; Boris W Kramer; John P Newnham; Alan H Jobe; Suhas G Kallapur Journal: Reprod Sci Date: 2015-07-07 Impact factor: 3.060